Cited by 196interferon and immune responsesT-cell and Retrovirus StudiesCytokine Signaling Pathways and Interactions

A Novel Interferon Regulatory Factor Family Transcription Factor, ICSAT/Pip/LSIRF, That Negatively Regulates the Activity of Interferon-Regulated Genes

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Yamagata, Tetsuya, Nishida, Junji, Tanaka, Tomoyuki, Sakai, Ryuichi, Mitani, Kinuko, Yoshida, Mitsuaki, Taniguchi, Tadatsugu, Yazaki, Yoshio +1 more·1996·Molecular and Cellular Biology

Abstract

We have isolated a novel cDNA clone encoding interferon (IFN) consensus sequence-binding protein in adult T-cell leukemia cell line or activated T cells (ICSAT); this protein is the human homolog of the recently cloned Pip/LSIRF. ICSAT is structurally most closely related to the previously cloned ICSBP, a member of the IFN regulatory factor (IRF) family of proteins that binds to interferon consensus sequences (ICSs) found in many promoters of the IFN-regulated genes. Among T-cell lines investigated, ICSAT was abundantly expressed in human T-cell leukemia virus type 1 (HTLV-1)-infected T cells. When the HTLV-1 tax gene was expressed or phorbol myristake acetate-A23187 stimulation was used, ICSAT expression was induced in Jurkat cells which otherwise do not express ICSAT. When the binding of ICSAT to four different ICSs was tested, the relative differences in binding affinities for those ICSs were determined. To study the functional role of ICSAT, we performed cotransfection experiments with the human embryonal carcinoma cell line N-Tera2. ICSAT was demonstrated to possess repressive function over the gene activation induced by IFN stimulation or by IRF-1 cotransfection. Such repressive function is similar to that seen in IRF-2 or ICSBP. However, we have found that ICSAT has a different repressive effect from that of IRF-2 or ICSBP in some IFN-responsive reporter constructs. These results suggest that a novel mechanism of gene regulation by "differential repression" is used by multiple members of repressor proteins with different repressive effects on the IFN-responsive genes.

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Crossref · 10.1128/mcb.16.4.1283

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Authors
Yamagata, T., Nishida, J., Tanaka, T., Sakai, R., Mitani, K., Yoshida, M., Taniguchi, T., Yazaki, Y., & Hirai, H.
Affiliations
  • The University of Tokyo (JP)
  • Teikyo University (JP)
  • Jichi Medical University (JP)
  • Institute of Medical Science, The University of Tokyo (JP)
Venue
Molecular and Cellular Biology
Published
1996-04-01
Volume
16
Issue
4
Pages
1283-1294
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2110972790
Cited by
196
References
75
Citations by year
2025: 3 · 2024: 2 · 2023: 1 · 2022: 6 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 1 · 2013: 8 · 2012: 4
FWCI
7.40
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
  • Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
Keywords
interferon-regulated genes, transcriptional repression, IRF8, IRF2, HTLV-1, tax gene, T-cell leukemia, gene expression regulation, repressor protein
MeSH terms
Calcimycin, Interferon Regulatory Factor-8, Interferon Regulatory Factor-4, Amino Acid Sequence, Animals, Base Sequence, Carrier Proteins, DNA-Binding Proteins, Gene Expression Regulation, Humans, Interferons, Lymphocyte Activation, Molecular Sequence Data, Repressor Proteins, T-Lymphocytes, Tetradecanoylphorbol Acetate, Transcription Factors, Tumor Cells, Cultured, Leukemia, T-Cell, Transcriptional Activation, Genes, pX, Consensus Sequence, Sequence Homology, Amino Acid, DNA, Complementary, Interferon Regulatory Factors

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