The DOI matches its Crossref record; 65 of the 68 references it cites were found in a registry or an index.
Reverse transcriptase activity of an intron encoded polypeptide
Open accessAbstract
A number of group II introns from eukaryotic organelles and prokaryotes contain open reading frames for polypeptides with homology to retroviral reverse transcriptases (RTs). We have used the yeast transposon (Ty) system to express ORFs for RTs from eukaryotic organelles. This includes the mitochondrial coxI intron i1 from the fungus Podospora anserina, the plastid petD intron from the alga Scenedesmus obliquus and the mitochondrial RTL gene from the alga Chlamydomonas reinhardtii. The ORFs were fused with the TYA ORF from the yeast retrotransposon Ty to produce virus-like particles in the recipient strains with detectable amounts of the RT-like polypeptides. Analysis of the heterologous gene products revealed biochemical evidence that the P. anserina intron encodes an RNA-directed DNA polymerase with properties typically found for RTs of viral or retrotransposable origin. In vitro assays showed that the intron encoded RT is sensitive to RT inhibitors such as N-ethylmaleimide and dideoxythymidine triphosphate but is insensitive against the DNA polymerase inhibitor aphidicolin. The direct biochemical evidence provided here supports the idea that intron encoded RTs are involved in intron transposition events.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Duplication of retrotransposons is a replicative event through transcribing themselves to RNA intermediate and then reversely transcribing back to DNA that has been demonstrated to be directed by RTase which is the key component of the retrotransposons.32”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [32]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Towbin, H., Staehelin, T., & Gordon, J. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4350 · Cited by 53,218
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311
Pushko, P., Pumpens, P., & Grens, E. (2013) · Intervirology · doi:10.1159/000346773 · Cited by 5,975
[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
Mullis, K. B., & Faloona, F. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)55023-6 · Cited by 5,762
Gilbert, W. (1978) · Nature · doi:10.1038/271501a0 · Cited by 2,300
Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of Rous Sarcoma Virus
TEMIN, H. M., & MIZUTANI, S. (1970) · Nature · doi:10.1038/2261211a0 · Cited by 2,128
Gorman, D. S., & Levine, R. P. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.6.1665 · Cited by 1,685
Research works printed but not linked: 15
- Boeke,J.D. (1989) In Berg,D.E. and Howe,M.M. (eds), Mobile DNA. American Society for Microbiology, Washington DC, pp. 335-375.
- Boeke,J.D. and Sandmeyer,S.B. (1991) In Broach,J.R., Jones,E.W. and Pringle,J. (eds), The Molecular and Cellular Biology of the Yeast Saccaromyces. pp. 193-261.
- Briihl,K.H. (1991) Ph.D. Thesis. Heinrich-Heine-Universitat, Dusseldorf.
- Gabriel,A. and Boeke,J.D. (1991) Proc. Natl Acad. Sci. USA, 88, 9794-9798.
- Gargouri,A., Lazowska,J. and Slonimski,P.P. (1983) In Schweyen,R., Wolf,K. and Kaudewitz,F. (eds), Mitochondria 1983. Walter de Gruyter, Berlin, pp. 259-268.
- Gorman,D.S. and Levine,R.P. (1965) Proc. Natl Acad. Sci. USA, 54, 1665-1669.
- Hizi,A., McGill,C. and Hughes,S.H. (1988) Proc. Natl Acad. Sci. USA, 85, 1218-1222.
- Jacobo-Molina,A. et al. (1993) Proc. NatlAcad. Sci. USA, 90, 6320-6324.
- Meunier,B., Tian,G.L., Macadre,C., Slonimski,P.P. and Lazowska,J. (1990) In Qualiariello,E., Papa,S., Palmieri,F. and Saccone,C. (eds), Structure, Function and Biogenesis ofEnergy Transfer Systems. Elsevier Science Publishers, Amsterdam, pp. 169-173.
- Sanger,F., Nicklen,S. and Coulsen,A.R. (1977) Proc. NatlAcad. Sci. USA, 74, 5463-5467.
- Soltis,D.A. and Skalka,M. (1988) Proc. Natl Acad. Sci. USA, 85, 3372-3376.
- Tanese,N., Roth,M. and Goff,S.P. (1985) Proc. NatlAcad. Sci. USA, 82, 4944-4948.
- Towbin,H., Staelin,T. and Gorden,J. (1979) Proc. Natl. Acad. Sci. USA, 76, 4350-4354
- Varmus,H. and Brown,P. (1989) In Berg,D.E. and Howe,M.M. (eds), Mobile DNA. American Society for Microbiology, Washington DC, pp. 53-108.
- Xu,H. and Boeke,J.D. (1990) Proc. NatlAcad. Sci. USA, 87, 8360-8364. Received on December 28, 1993; revised on February 4, 1994
Cited by
Sorted by citations
Host-selective toxins produced by the plant pathogenic fungusAlternaria alternata
Tsuge, T., Harimoto, Y., Akimitsu, K., Ohtani, K., Kodama, M., Akagi, Y., Egusa, M., Yamamoto, M., & Otani, H. (2012) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2012.00350.x · Cited by 406
“This intron, known as a self-splicing group II intron, catalyses its own splicing (Leaver & Gray, 1982), and many of these introns have been reported to contain ORFs encoding polypeptides (Fassbender et al., 1994).”
BackgroundThe give-and-take of DNA: horizontal gene transfer in plants
Bock, R. (2009) · Trends in Plant Science · doi:10.1016/j.tplants.2009.10.001 · Cited by 276
Group II Introns: Structure and Catalytic Versatility of Large Natural Ribozymes
Lehmann, K., & Schmidt, U. (2003) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/713609236 · Cited by 170
“anserina (Faßbender et al., 1994).”
Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus
Akimitsu, K., Peever, T. L., & Timmer, L. W. (2003) · Molecular Plant Pathology · doi:10.1046/j.1364-3703.2003.00189.x · Cited by 143
“This intron, known as a self-splicing group II intron, catalyses its own splicing (e.g. Leaver and Gray, 1982), and many of these introns have been reported to contain open reading frames encoding polypeptides (e.g. Fassbender et al., 1994).”
BackgroundBock, R. (2017) · Annual Review of Genetics · doi:10.1146/annurev-genet-120215-035329 · Cited by 117
Genes, mitochondria and aging in filamentous fungi
Osiewacz, H. D. (2002) · Ageing Research Reviews · doi:10.1016/s1568-1637(02)00010-7 · Cited by 106
Liere, K., & Link, G. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.6.917 · Cited by 86
de Cambiaire, J.-C., Otis, C., Lemieux, C., & Turmel, M. (2006) · BMC Evolutionary Biology · doi:10.1186/1471-2148-6-37 · Cited by 81
“This intron harbours in its domain IV an ORF encoding a reverse transcriptase [31] with the typical reverse transcriptase, maturase and nuclease domains [32].”
BackgroundBorghouts, C., Kimpel, E., & Osiewacz, H. D. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.20.10768 · Cited by 79
Ty1 and Ty5 ofSaccharomyces cerevisiae
Voytas, D. F., & Boeke, J. D. (2007) · Wiley eBooks · doi:10.1128/9781555817954.ch26 · Cited by 79
Lazowska, J., Meunier, B., & Macadré, C. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06823.x · Cited by 72
Mitochondrial functions and aging
Osiewacz, H. D. (2002) · Gene · doi:10.1016/s0378-1119(01)00804-6 · Cited by 70
Aging in fungi: role of mitochondria in Podospora anserina
Osiewacz, H. D. (2002) · Mechanisms of Ageing and Development · doi:10.1016/s0047-6374(01)00421-3 · Cited by 70
Sensitivity to Alternaria alternata toxin in citrus because of altered mitochondrial RNA processing
Ohtani, K., Yamamoto, H., & Akimitsu, K. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.042448499 · Cited by 69
Silar, P., Koll, F., & Rossignol, M. (1997) · Genetics · doi:10.1093/genetics/145.3.697 · Cited by 55
Mitochondrial-nuclear interactions and lifespan control in fungi
Osiewacz, H. D., & Kimpel, E. (1999) · Experimental Gerontology · doi:10.1016/s0531-5565(99)00063-7 · Cited by 45
Function of chloroplast RNA-binding proteins
Jacobs, J., & Kück, U. (2010) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-010-0523-3 · Cited by 43
“an in vitro assay to have RNA-directed DNA polymerase activity with a sensitivity to RT inhibitors [95].”
Self-citationXu, T., Qin, S., Hu, Y., Song, Z., Ying, J., Li, P., Dong, W., Zhao, F., Yang, H., & Bao, Q. (2016) · DNA Research · doi:10.1093/dnares/dsw023 · Cited by 37
“Duplication of retrotransposons is a replicative event through transcribing themselves to RNA intermediate and then reversely transcribing back to DNA that has been demonstrated to be directed by RTase which is the key component of the retrotransposons.(32) However, its integration into the A.”
BackgroundKoll, F., Boulay, J., Belcour, L., & d’Aubenton-Carafa, Y. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.9.1734 · Cited by 36
Intramolecular cross-overs generate deleted mitochondrial DNA molecules in Podospora anserina
Vierny, C., Boulay, J., & Briand, J.-F. (1997) · Current Genetics · doi:10.1007/s002940050191 · Cited by 35
Metabolism and aging in the filamentous fungus Podospora anserina
Osiewacz, H. D., & Stumpferl, S. W. (2001) · Archives of Gerontology and Geriatrics · doi:10.1016/s0167-4943(01)00096-6 · Cited by 33
Cell degeneration in the model system Podospora anserina
Silar, P., Lalucque, H., & Vierny, C. (2001) · Biogerontology · doi:10.1023/a:1010000816277 · Cited by 31
Origin and Evolution of Chloroplasts: Current Status and Future Perspectives
Kowallik, K. V. (1997) · Springer eBooks · doi:10.1007/978-3-642-60885-8_1 · Cited by 31
Vierny, C., Boulay, J., Begel, O., & Silar, P. (1997) · Current Genetics · doi:10.1007/s002940050192 · Cited by 28
Sandmeyer, S. B., & Menees, T. M. (1996) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-80145-7_9 · Cited by 27
“…including those of the LINE-like element CREI from the trypanosomatid Crithidia fasciculata (GABRIEL and BoEKE 1991), the human LINE element L1 .2A (MATHIAS et al. 1991), the cox/ intron il from the fungus Podospora anserina (FASSBENDER et al. 1994), and duck hepatitis virus 8 (TAVIS et al. 1994).”
BackgroundArtificial Genes for Chimeric Virus-Like Particles
Khudyakov, Y. E., & Fields, H. A. (2002) · CRC Press eBooks · doi:10.1201/9781420040166-12 · Cited by 27
Mitochondrial Oxidative Stress and Aging in the Filamentous Fungus Podospora anserina
Osiewacz, H. D., & Borghouts, C. (2000) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2000.tb06633.x · Cited by 26
Freyer, R., Neckermann, K., Maier, R. M., & Kössel, H. (1995) · Current Genetics · doi:10.1007/bf00314451 · Cited by 25
Recombination:Organelle DNA of Plants and Fungi: Inheritance and Recombination
Röhr, H., Kües, U., & Ståhl, U. (1999) · Progress in botany · doi:10.1007/978-3-642-59940-8_2 · Cited by 24
In and out: Evolution of viral sequences in the mitochondrial genomes of legumes (Fabaceae)
Choi, I., Wojciechowski, M. F., Ruhlman, T. A., & Jansen, R. K. (2021) · Molecular Phylogenetics and Evolution · doi:10.1016/j.ympev.2021.107236 · Cited by 20
Osiewacz, H. D., & Borghouts, C. (2000) · Experimental Gerontology · doi:10.1016/s0531-5565(00)00142-x · Cited by 20
Purifying Selection Masks the Mutational Flexibility of HIV-1 Reverse Transcriptase
Smith, R. A., Anderson, D. J., & Preston, B. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m314038200 · Cited by 16
Nedelcu, A. M. (1998) · Journal of Phycology · doi:10.1046/j.1529-8817.1998.340016.x · Cited by 16
“To date, although rather few green algal mitochondrial genomes have been investigated, an rtl has been found only in C. reinhardtii (Boer and Gray 1988a); however, the overexpressed product of the C. reinhardtii rtl does not appear to have a reverse transcriptase activity (Faßbender et al. 1994).”
BackgroundMobile Genetic Elements in Mycelial Fungi
Pöggeler, S., & Kempken, F. (2004) · Springer eBooks · doi:10.1007/978-3-662-07426-8_10 · Cited by 16
Osiewacz, H. D., & Schürmanns, L. (2021) · Journal of Fungi · doi:10.3390/jof7040263 · Cited by 15
“Significantly, although not formally proven, a protein with reverse transcriptase activity encoded by the pl-intron may be involved in transposition processes [44].”
BackgroundSalvo, J. L. G., Rodeghier, B., Rubin, A., & Troischt, T. (1998) · Fungal Genetics and Biology · doi:10.1006/fgbi.1997.1030 · Cited by 15
Stability of the Mitochondrial Genome of Podospora anserina and Its Genetic Control
Belcour, L., Sainsard‐Chanet, A., Vierny, C., & Picard-Bennoun, M. (1999) · Springer eBooks · doi:10.1007/978-3-642-59884-5_16 · Cited by 15
Role of transposable elements in age-related genomic instability
Nikitin, A. G., & Reis, R. J. S. (1997) · Genetics Research · doi:10.1017/s0016672397002772 · Cited by 14
Aging and mitochondrial dysfunction in the filamentous fungus Podospora anserina
Osiewacz, H. D. (2003) · Topics in current genetics · doi:10.1007/978-3-540-37005-5_2 · Cited by 13
Sellem, C. H., Begel, O., & Sainsard‐Chanet, A. (2000) · Current Genetics · doi:10.1007/s002940050003 · Cited by 10
“The only known reverse transcriptase activity in mitochondria of the s wild-type strain is that encoded in the group-II intron a (Sainsard-Chanet et al. 1993; Fassbender et al. 1994).”
Nedelcu, A. M., & Lee, R. W. (2006) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-48204-5_5 · Cited by 10
Senescence in Podospora anserina
Osiewacz, H. D., & Scheckhuber, C. Q. (2002) · doi:10.1201/9780203910719-6 · Cited by 9
Reverse Transcriptase Activities in Mycelial Fungi
Faßbender, S., & Kück, U. (1995) · Springer eBooks · doi:10.1007/978-3-662-10364-7_15 · Cited by 8
Self-citationSenescence in Podospora anserina
Osiewacz, H. D., & Scheckhuber, C. Q. (2002) · CRC Press eBooks · doi:10.1201/9780203910719.ch4 · Cited by 6
Hamann, A., & Osiewacz, H. D. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-811353-0.00031-2 · Cited by 6
Osiewacz, H. D., & Hamann, A. (2006) · Springer eBooks · doi:10.1007/3-540-28135-5_10 · Cited by 5
Aging and Longevity in the Filamentous Fungus Podospora anserina
Osiewacz, H. D. (2003) · Springer eBooks · doi:10.1007/978-94-017-0671-1_2 · Cited by 3
Nuclear-Mitochondrial Interactions Involved in Biological Ageing
Osiewacz, H. D. (1996) · Springer eBooks · doi:10.1007/978-1-4615-5889-7_4 · Cited by 2
Podospora anserina bibliography n° 10 - Additions
Debuchy, R. (2003) · Fungal Genetics Reports · doi:10.4148/1941-4765.1161 · Cited by 1
Ciriacy, M. (1995) · Springer eBooks · doi:10.1007/978-3-662-10364-7_14 · Cited by 1
Self-citationKück, P. D. U. (2005) · Springer eBooks · doi:10.1007/3-540-26469-8_7
Role of Mitochondria in Mechanisms of Fungal Senescence and Longevity
Osiewacz, H. D. (2026) · The mycota · doi:10.1007/978-3-032-15548-1_4
Metadata
- Authors
- Fassbender, S., Brühl, K. H., Ciriacy, M., & Kück, U.
- Affiliations
- Ruhr University Bochum (DE)
- ORCID
- Kück, U.
- Venue
- The EMBO Journal
- Published
- 1994-05-01
- Volume
- 13
- Issue
- 9
- Pages
- 2075-2083
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC395058
- OpenAlex
- W141846053
- Cited by
- 52
- References
- 68
- Citations by year
- 2026: 1 · 2021: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2012: 1
- FWCI
- 6.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- group II introns, reverse transcriptase, Podospora anserina, Chlamydomonas reinhardtii, Ty retrotransposon, N-ethylmaleimide sensitivity
- MeSH terms
- Ascomycota, Base Sequence, Cloning, Molecular, DNA Transposable Elements, DNA, Fungal, Genetic Code, Introns, Molecular Sequence Data, Peptides, Recombinant Fusion Proteins, RNA-Directed DNA Polymerase, Open Reading Frames, Eukaryota
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
