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Reverse transcriptase activity of an intron encoded polypeptide
Open accessAbstract
A number of group II introns from eukaryotic organelles and prokaryotes contain open reading frames for polypeptides with homology to retroviral reverse transcriptases (RTs). We have used the yeast transposon (Ty) system to express ORFs for RTs from eukaryotic organelles. This includes the mitochondrial coxI intron i1 from the fungus Podospora anserina, the plastid petD intron from the alga Scenedesmus obliquus and the mitochondrial RTL gene from the alga Chlamydomonas reinhardtii. The ORFs were fused with the TYA ORF from the yeast retrotransposon Ty to produce virus-like particles in the recipient strains with detectable amounts of the RT-like polypeptides. Analysis of the heterologous gene products revealed biochemical evidence that the P. anserina intron encodes an RNA-directed DNA polymerase with properties typically found for RTs of viral or retrotransposable origin. In vitro assays showed that the intron encoded RT is sensitive to RT inhibitors such as N-ethylmaleimide and dideoxythymidine triphosphate but is insensitive against the DNA polymerase inhibitor aphidicolin. The direct biochemical evidence provided here supports the idea that intron encoded RTs are involved in intron transposition events.
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“Duplication of retrotransposons is a replicative event through transcribing themselves to RNA intermediate and then reversely transcribing back to DNA that has been demonstrated to be directed by RTase which is the key component of the retrotransposons.32”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [32]
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Sorted by citations
Host-selective toxins produced by the plant pathogenic fungusAlternaria alternata
Tsuge, T., Harimoto, Y., Akimitsu, K., Ohtani, K., Kodama, M., Akagi, Y., Egusa, M., Yamamoto, M., & Otani, H. (2012) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2012.00350.x · Cited by 406
“This intron, known as a self-splicing group II intron, catalyses its own splicing (Leaver & Gray, 1982), and many of these introns have been reported to contain ORFs encoding polypeptides (Fassbender et al., 1994).”
BackgroundThe give-and-take of DNA: horizontal gene transfer in plants
Bock, R. (2009) · Trends in Plant Science · doi:10.1016/j.tplants.2009.10.001 · Cited by 276
Group II Introns: Structure and Catalytic Versatility of Large Natural Ribozymes
Lehmann, K., & Schmidt, U. (2003) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/713609236 · Cited by 170
“anserina (Faßbender et al., 1994).”
Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus
Akimitsu, K., Peever, T. L., & Timmer, L. W. (2003) · Molecular Plant Pathology · doi:10.1046/j.1364-3703.2003.00189.x · Cited by 143
“This intron, known as a self-splicing group II intron, catalyses its own splicing (e.g. Leaver and Gray, 1982), and many of these introns have been reported to contain open reading frames encoding polypeptides (e.g. Fassbender et al., 1994).”
BackgroundBock, R. (2017) · Annual Review of Genetics · doi:10.1146/annurev-genet-120215-035329 · Cited by 117
Metadata
- Authors
- Fassbender, S., Brühl, K. H., Ciriacy, M., & Kück, U.
- Affiliations
- Ruhr University Bochum (DE)
- ORCID
- Kück, U.
- Venue
- The EMBO Journal
- Published
- 1994-05-01
- Volume
- 13
- Issue
- 9
- Pages
- 2075-2083
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC395058
- OpenAlex
- W141846053
- Cited by
- 52
- References
- 68
- Citations by year
- 2026: 1 · 2021: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2012: 1
- FWCI
- 6.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- group II introns, reverse transcriptase, Podospora anserina, Chlamydomonas reinhardtii, Ty retrotransposon, N-ethylmaleimide sensitivity
- MeSH terms
- Ascomycota, Base Sequence, Cloning, Molecular, DNA Transposable Elements, DNA, Fungal, Genetic Code, Introns, Molecular Sequence Data, Peptides, Recombinant Fusion Proteins, RNA-Directed DNA Polymerase, Open Reading Frames, Eukaryota
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