Cited by 52Chromosomal and Genetic VariationsRNA Research and SplicingRNA and protein synthesis mechanisms

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Reverse transcriptase activity of an intron encoded polypeptide

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Fassbender, S., Brühl, K.H., Ciriacy, M., Kück, U.·1994·The EMBO Journal

DOI 10.1002/j.1460-2075.1994.tb06482.xPMC PMC395058

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Abstract

A number of group II introns from eukaryotic organelles and prokaryotes contain open reading frames for polypeptides with homology to retroviral reverse transcriptases (RTs). We have used the yeast transposon (Ty) system to express ORFs for RTs from eukaryotic organelles. This includes the mitochondrial coxI intron i1 from the fungus Podospora anserina, the plastid petD intron from the alga Scenedesmus obliquus and the mitochondrial RTL gene from the alga Chlamydomonas reinhardtii. The ORFs were fused with the TYA ORF from the yeast retrotransposon Ty to produce virus-like particles in the recipient strains with detectable amounts of the RT-like polypeptides. Analysis of the heterologous gene products revealed biochemical evidence that the P. anserina intron encodes an RNA-directed DNA polymerase with properties typically found for RTs of viral or retrotransposable origin. In vitro assays showed that the intron encoded RT is sensitive to RT inhibitors such as N-ethylmaleimide and dideoxythymidine triphosphate but is insensitive against the DNA polymerase inhibitor aphidicolin. The direct biochemical evidence provided here supports the idea that intron encoded RTs are involved in intron transposition events.

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Authors: Fassbender, S., Brühl, K. H., Ciriacy, M., & Kück, U.
Affiliations: Ruhr University Bochum (DE)
ORCID: Kück, U.
Venue: The EMBO Journal
Publisher: Springer Science and Business Media LLC
Published: 1994-05-01
Volume: 13
Issue: 9
Pages: 2075-2083
ISSN: 0261-4189 (ISSN-L)
1460-2075
DOI: 10.1002/j.1460-2075.1994.tb06482.x
PMC: PMC395058
OpenAlex: W141846053
Cited by: 52
References: 68
Citations by year: 2026: 1 · 2021: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2012: 1
FWCI: 6.93
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: The EMBO Journal (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Deutsche Forschungsgemeinschaft
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords: group II introns, reverse transcriptase, Podospora anserina, Chlamydomonas reinhardtii, Ty retrotransposon, N-ethylmaleimide sensitivity
MeSH terms: Ascomycota, Base Sequence, Cloning, Molecular, DNA Transposable Elements, DNA, Fungal, Genetic Code, Introns, Molecular Sequence Data, Peptides, Recombinant Fusion Proteins, RNA-Directed DNA Polymerase, Open Reading Frames, Eukaryota

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Reverse transcriptase activity of an intron encoded polypeptide

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