A di‐leucine motif mediates endocytosis and basolateral sorting of macrophage IgG Fc receptors in MDCK cells.
Open accessAbstract
An important function of the low affinity IgG Fc receptor FcRII-B2 (FcR) on macrophages is the internalization of soluble antigen-antibody complexes for lysosomal degradation. Most endocytic receptors possess tyrosine-containing cytoplasmic determinants required for endocytosis. In many proteins, signals which overlap with the endocytosis determinant and share the same critical tyrosine residue also mediate basolateral sorting in the trans-Golgi network of epithelial cells. Despite the presence of two tyrosine residues in the FcR cytosolic domain, neither one is absolutely required for coated pit localization or basolateral targeting. Nevertheless, a short domain of 13 residues containing one of the non-critical tyrosine residues mediates endocytosis and basolateral delivery. Alanine scan mutagenesis of this region now revealed a critical role of a leucine-leucine motif in both events. These findings suggest that endocytosis and basolateral sorting can be mediated by both tyrosine- and di-leucine-based signals and confirm the close relationship between the two determinants already observed for 'classical' tyrosine-dependent motifs.
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The dileucine motif mediates endocytosis and sorting of many receptors.
“the cytoplasmic domain of LRAD3 contains a conserved dileucine motif which has been demonstrated to mediate endocytosis and sorting of many receptors, including CD3 (Letourneur and Klausner, 1992), IgG Fc receptors (Hunziker and Fumey, 1994), the IL-6 receptor (Dittrich et al., 1996), and CD44 (Sheikh and Isacke, 1996).”
From LRAD3, A Novel Low-Density Lipoprotein Receptor Family Member That Modulates Amyloid Precursor Protein Trafficking (2011) · cites this paper as Hunziker and Fumey, 1994
The cytoplasmic domain of LRAD3 contains a conserved dileucine motif.
“Instead, the cytoplasmic domain of LRAD3 contains a conserved dileucine motif which has been demonstrated to mediate endocytosis and sorting of many receptors, including CD3 (Letourneur and Klausner, 1992), IgG Fc receptors (Hunziker and Fumey, 1994), the IL-6 receptor (Dittrich et al., 1996), and CD44 (Sheikh and Isacke, 1996).”
From LRAD3, A Novel Low-Density Lipoprotein Receptor Family Member That Modulates Amyloid Precursor Protein Trafficking (2011) · cites this paper as Hunziker and Fumey, 1994
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Collawn, J. F., Stangel, M., Kuhn, L. A., Esekogwu, V., Jing, S., Trowbridge, I. S., & Tainer, J. A. (1990) · Cell · doi:10.1016/0092-8674(90)90509-d · Cited by 518 · see citing works
Internalization and degradation of macrophage Fc receptors bound to polyvalent immune complexes.
Mellman, I., & Plutner, H. (1984) · The Journal of cell biology · doi:10.1083/jcb.98.4.1170 · Cited by 251 · see citing works
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- Hibbs,M.L., Walker,I.D., Kirszbaum,L., Pietersz,G.A., Deacon,N.J., Chambers,G.W., Mckenzie,I.F.C. and Hogarth,P.M. (1986) Proc. Natl Acad. Sci. USA, 83, 6980-6984.
- McGraw,T.E. and Maxfield,F.R. (1990) Cell. Regul., 1, 369-377.
- Mellman,I., Miettinen,H., Male,P. and Hunziker,W. (1992) In Courtoy,P.J. (ed.), Endocvtosis. From Cell Biology to Health, Disease and Therapy. NATO ASI Series, Vol. H 62, pp. 309-316. Springer Verlag, Berlin.
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Coordinated protein sorting, targeting and distribution in polarized cells
Mellman, I., & Nelson, W. J. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2525 · Cited by 521 · see citing works
Lysosome biogenesis in health and disease
Bajaj, L., Lotfi, P., Pal, R., Ronza, A. D., Sharma, J., & Sardiello, M. (2019) · Journal of Neurochemistry · doi:10.1111/jnc.14564 · Cited by 153 · see citing works
Ranganathan, S., Noyes, N. C., Migliorini, M., Winkles, J. A., Battey, F. D., Hyman, B. T., Smith, E., Yepes, M., Mikhailenko, I., & Strickland, D. K. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.5065-10.2011 · Cited by 49 · see citing works
“…of LRAD3 contains a conserved dileucine motif that has been demonstrated to mediate endocytosis and sorting of many receptors, including CD3 (Letourneur and Klausner, 1992), IgG Fc receptors (Hunziker and Fumey, 1994), the IL-6 receptor (Dittrich et al., 1996), and CD44 (Sheikh and Isacke, 1996).”
Background
- Authors
- Hunziker, W., & Fumey, C.
- Affiliations
- University of Lausanne (CH)
- ORCID
- Hunziker, W.
- Venue
- The EMBO Journal
- Published
- 1994-07-01
- Volume
- 13
- Issue
- 13
- Pages
- 2963-2969
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC395183
- OpenAlex
- W213218024
- Cited by
- 252
- References
- 2
- Citations by year
- 2026: 3 · 2024: 4 · 2023: 2 · 2021: 4 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 4 · 2012: 6
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- dileucine motif, endocytosis, basolateral sorting, MDCK cells, trans-Golgi network, tyrosine residues
- MeSH terms
- Amino Acid Sequence, Antigen-Antibody Complex, Biological Transport, Cell Line, Cell Membrane, Cytoplasm, Endocytosis, Golgi Apparatus, Immunoglobulin G, Immunoglobulin Fab Fragments, Kinetics, Leucine, Macrophages, Molecular Sequence Data, Mutagenesis, Site-Directed, Cell Polarity, Receptors, IgG
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