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Cited by 251Monoclonal and Polyclonal Antibodies ResearchCellular transport and secretionPhagocytosis and Immune Regulation

Internalization and degradation of macrophage Fc receptors bound to polyvalent immune complexes.

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Mellman, I, Plutner, H·1984·The Journal of cell biology
DOI 10.1083/jcb.98.4.1170PMC PMC2113248
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Abstract

We have studied the Fc receptor-mediated pinocytosis of immunoglobulin G (IgG)-containing immune complexes by mouse macrophages. IgG complexes were formed from affinity-purified rabbit dinitrophenyl IgG and dinitrophenyl modified BSA at molar ratios of 2.5-10:1. Both the specificity of binding and the fate of internalized receptors were analyzed using monoclonal and polyclonal anti-Fc receptor antibodies. Based on the susceptibility of surface-bound ligand to release by proteolysis, we have found that at 37 degrees C, 125I-labeled IgG complexes were rapidly internalized (t1/2 less than 2 min) and delivered to lysosomes; acid-soluble 125I was detectable in the growth medium within 5-10 min of uptake. However, kinetic evidence indicated that Fc receptors were not efficiently re-used for multiple rounds of ligand uptake. Instead, macrophages that were exposed continuously to saturating concentrations of IgG complexes exhibited a selective and largely irreversible removal of Fc receptors from the plasma membrane. This loss of surface receptors correlated with an increased rate of receptor turnover, determined by immune precipitation of Fc receptors from 125I-labeled macrophages. Thus, in contrast to the results obtained in the accompanying paper (I. Mellman, H. Plutner, and P. Ukkonen, 1984, J. Cell Biol. 98:1163-1169) using a monovalent ligand, these data indicate that the interaction of Fc receptors with polyvalent complexes leads to the degradation of both ligand and receptor following their delivery to lysosomes.

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  • Target-mediated clearance refers to the elimination of mAbs through antigen-specific interactions; after mAbs bind a specific antigen, they may be internalized and catabolized through lysosomal degradation as an antibody-antigen complex.

    “Target‐mediated clearance refers to the elimination of mAbs through its antigen‐specific interactions. After mAbs bind to a specific antigen (soluble or membrane bound) they may be internalized and catabolized through lysosomal degradation as an antibody–antigen complex.35”

    From Tutorial on monoclonal antibody pharmacokinetics and its considerations in early development (2018) · cites this paper as [35]

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Authors
Mellman, I., & Plutner, H.
ORCID
Mellman, I
Venue
The Journal of cell biology
Publisher
Rockefeller University Press
Published
1984-04-01
Volume
98
Issue
4
Pages
1170-1177
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.98.4.1170
PMC
PMC2113248
OpenAlex
W2075507579
Cited by
251
References
34
Citations by year
2026: 3 · 2025: 3 · 2024: 6 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 4 · 2016: 3 · 2015: 3 · 2014: 3 · 2013: 5 · 2012: 4
FWCI
21.35
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
Keywords
Fc receptors, macrophages, immune complexes, receptor internalization, receptor degradation, pinocytosis, lysosomes, receptor turnover, IgG, ligand uptake, receptor recycling
MeSH terms
Animals, Antibodies, Monoclonal, Antigen-Antibody Complex, Cell Line, Immunoglobulin G, Kinetics, Macrophages, Microscopy, Fluorescence, Pinocytosis, Receptors, Fc, Mice

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