Phenotypic non-equivalence of murine (monocyte-) macrophage cells in biomaterial and inflammatory models
Open accessAbstract
Cells of the mononuclear phagocytic system including monocytes and macrophages (e.g., pooled human monocytes, bone marrow‐derived macrophages, etc.) are often employed for in vitro assessment of novel biomaterials and to assay anti‐inflammatory drug activity. In this context, numerous macrophage cells are treated interchangeably in the literature despite a lack of demonstrated equivalence among immortalized cell lines and further, between cell lines and primary‐derived macrophages of different species. Three murine (monocyte‐) macrophage cell lines (IC‐21, J774A.1, and RAW 264.7), commonly utilizedin biomaterial and pharmaceutical screening research, have been compared with primary‐derived murine bone marrow macrophages. Significant differences were discovered in the expression of cell surface proteins requisite for cell adhesion and activation among cell lines and primary‐derived cells as well as between the different cell lines. Results demonstrate activation but with reduced cytokine expression to chemical stimulus (lipopolysaccharide) by cell lines compared with that of primary‐derived macrophages. Limited correlation between cultured primary and immortalized cells in cytokine production, phenotype and intrinsic activation states has relevance to fidelity for in vitro testing. These differences warrant justification for selection of various cell lines for specific assay purposes, and merit caution if comparisons to primary cell types (i.e., for biocompatibility) are required. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
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Several physical factors of ECMs, including stiffness and topography, influence macrophage polarization and determine outcomes of wound healing and tissue remodeling.
“Several physical factors of ECMs have been evidenced to influence macrophages polarization and determine the outcomes of wound healing and tissue remodeling, including stiffness, topography and so on [[27], [28], [29]].”
From Substrate stiffness modulates bone marrow-derived macrophage polarization through NF-κB signaling pathway (2020) · cites this paper as [28]
The section on cytokine mRNA expression on different surfaces reports that material type affected some macrophage cytokine responses in vitro. The studied surfaces were model biomaterials, however, and the paper does not establish that ECM stiffness or topography influences macrophage polarization or determines wound-healing and tissue-remodeling outcomes. Page numbers were not available in our copy. (LLM, conf=0.86)
Experimental — accuracy not yet measuredMΦ cell lines are not functionally equivalent to primary MΦs.
“While MΦ cell lines are useful and easy to manipulate, they are not functionally equivalent to primary MΦs [25].”
From Macrophage functional polarization (M1/M2) in response to varying fiber and pore dimensions of electrospun scaffolds (2013) · cites this paper as [25]
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- Authors
- Chamberlain, L. M., Godek, M. L., Gonzalez‐Juarrero, M., & Grainger, D. W.
- Affiliations
- University of Utah (US)
- Colorado State University (US)
- Corresponding author
- Grainger, David W.
- ORCID
- Grainger, David W.
- Venue
- Journal of Biomedical Materials Research Part A
- Publisher
- Wiley
- Published
- 2008-03-20
- Volume
- 88A
- Issue
- 4
- Pages
- 858-871
- ISSN
- 1549-3296 (ISSN-L)
- 1552-4965
- PMC
- PMC10031642
- OpenAlex
- W2127236332
- Cited by
- 112
- References
- 49
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 8 · 2023: 6 · 2022: 6 · 2021: 8 · 2020: 7 · 2019: 6 · 2018: 4 · 2017: 7 · 2016: 9 · 2015: 12 · 2014: 2 · 2013: 7 · 2012: 7
- FWCI
- 2.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Biomedical Materials Research Part A (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- U.S. Food and Drug Administration
- National Institute of Biomedical Imaging and Bioengineering (EB000894, R01EB000894)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- RAW 264.7, cell line heterogeneity, macrophage activation, cytokine expression, lipopolysaccharide stimulation, adhesion molecules, anti-inflammatory drug screening
- MeSH terms
- Animals, Biocompatible Materials, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Female, Humans, Lipopolysaccharides, Macrophages, Materials Testing, Mice, Inbred C57BL, Models, Biological, Phenotype, Surface Properties, Biomarkers, Cytokines, Cell Culture Techniques, Mice
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