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Cited by 83Tissue Engineering and Regenerative MedicineBone Tissue Engineering MaterialsHydrogels: synthesis, properties, applications

Epidermal and dermal integration into sphere‐templated porous poly(2‐hydroxyethyl methacrylate) implants in mice

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Fukano, Y., Usui, M. L., Underwood, R. A., Isenhath, S., Marshall, A. J., Hauch, K. D., Ratner, B. D., Olerud, J. E. +1 more·2010·Journal of Biomedical Materials Research Part A
DOI 10.1002/jbm.a.32798PMC PMC2998188
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Abstract

Percutaneous medical devices remain susceptible to infection and failure. We hypothesize that healing of the skin into the percutaneous device will provide a seal, preventing bacterial attachment, biofilm formation, and subsequent device failure. Porous poly(2‐hydroxyethyl methacrylate) [poly(HEMA)] with sphere‐templated pores (40 μm) and interconnecting throats (16 μm) were implanted in normal C57BL/6 mice for 7, 14, and 28 days. Poly(HEMA) was either untreated, keeping the surface nonadhesive for cells and proteins, or modified with carbonyldiimidazole (CDI) or CDI reacted with laminin 332 to enhance adhesion. No clinical signs of infection were observed. Epidermal and dermal response within the poly(HEMA) pores was evaluated using light and transmission electron microscopy. Cells (keratinocytes, fibroblasts, endothelial cells, inflammatory cells) and basement membrane proteins (laminin 332, β4 integrin, type VII collagen) could be demonstrated within the poly(HEMA) pores of all implants. Blood vessels and dermal collagen bundles were evident in all of the 14‐ and 28‐day implants. Fibrous capsule formation and permigration were not observed. Sphere‐templated polymers with 40 μm pores demonstrate an ability to recapitulate key elements of both the dermal and the epidermal layers of skin. Our morphological findings indicate that the implant model can be used to study the effects of biomaterial pore size, pore interconnect (throat) size, and surface treatments on cutaneous biointegration. Further, this model may be used for bacterial challenge studies. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.

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  • Materials with pores of 30-40 mm healed with minimal fibrosis and highest vascularity, and these pores were heavily infiltrated by macrophages but not foreign body giant cells.

    “It was noted that those materials possessing pores of 30–40 mm healed with minimal fibrosis and the highest vascularity [149]. These pores were also found to be heavily infiltrated by macrophages, but not foreign body giant cells.”

    From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [149]

  • Porous biomaterials of the same composition as non-porous forms that elicit foreign body reaction and encapsulation heal with less encapsulation and more vascularity when implanted into dermis or cardiac tissues.

    “Recent studies have demonstrated that porous biomaterials of the same composition as those which, in their non-porous form, elicit an FBR and encapsulation heal with less encapsulation and more vascularity when implanted into either the dermis or into cardiac tissues [147–149].”

    From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [149]

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Research works printed but not linked: 6

  1. [5]Mermel Correction: Prevention of intravascular catheter-related infections Ann Intern Med 2000 133 395 10691590
  2. [30]Marshall AJ Ratner BD Quantitative characterization of sphere-templated porous biomaterials AIChE Journal 2005 51 4 1221 1232
  3. [49]Bryant R Wound repair: a review J Enterostomal Ther 1987 14 6 262 266 2447136
  4. [52]Mellman I Warren G The road taken: past and future foundations of membrane traffic Cell 2000 100 1 99 112 10647935
  5. [57]Odland G Ross R Human wound repair. I. Epidermal regeneration J Cell Biol 1968 39 1 135 151 5678445 PMC2107509
  6. [58]Ross R Odland G Human wound repair. II. Inflammatory cells, epithelial-mesenchymal interrelations, and fibrogenesis J Cell Biol 1968 39 1 152 168 5678446 PMC2107500

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Authors
Fukano, Y., Usui, M. L., Underwood, R. A., Isenhath, S., Marshall, A. J., Hauch, K. D., Ratner, B. D., Olerud, J. E., & Fleckman, P.
Affiliations
University of Washington (US)
Corresponding author
Usui, M. L.
ORCID
Venue
Journal of Biomedical Materials Research Part A
Publisher
Wiley
Published
2010-08-02
Volume
94A
Issue
4
Pages
1172-1186
ISSN
  • 1549-3296 (ISSN-L)
  • 1552-4965
DOI
10.1002/jbm.a.32798
PMC
PMC2998188
OpenAlex
W2042448131
Cited by
83
References
54
Citations by year
2026: 3 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 9 · 2019: 4 · 2018: 2 · 2017: 7 · 2016: 5 · 2015: 11 · 2014: 7 · 2013: 8 · 2012: 10
FWCI
7.76
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Journal of Biomedical Materials Research Part A (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK 59221, R01DK059221)
  • National Institute of Biomedical Imaging and Bioengineering (EB-004422, R01EB004422)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
poly(2-hydroxyethyl methacrylate), laminin-5, bacterial adhesion, biofilm inhibition, basement membrane proteins, ITGB4, type VII collagen, keratinocytes, fibroblasts, endothelial cells, implant-associated infection
MeSH terms
Epidermal Cells, Animals, Epidermis, Immunohistochemistry, Macrophages, Methacrylates, Mice, Inbred C57BL, Phenotype, Porosity, Tissue Fixation, Implants, Experimental, Dermis, Mice

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