Hypermineralization and High Osteocyte Lacunar Density in Osteogenesis Imperfecta Type V Bone Indicate Exuberant Primary Bone Formation
Open accessAbstract
In contrast to “classical” forms of osteogenesis imperfecta (OI) types I to IV, caused by a mutation in COL1A1/A2, OI type V is due to a gain-of-function mutation in the IFITM5 gene, encoding the interferon-induced transmembrane protein 5, or bone-restricted interferon-inducible transmembrane (IFITM)-like protein (BRIL). Its phenotype distinctly differs from OI types I to IV by absence of blue sclerae and dentinogenesis imperfecta, by the occurrence of ossification disorders such as hyperplastic callus and forearm interosseous membrane ossification. Little is known about the impact of the mutation on bone tissue/material level in untreated and bisphosphonate-treated patients. Therefore, investigations of transiliac bone biopsy samples from a cohort of OI type V children (n = 15, 8.7 ± 4 years old) untreated at baseline and a subset (n = 8) after pamidronate treatment (2.6 years in average) were performed. Quantitative backscattered electron imaging (qBEI) was used to determine bone mineralization density distribution (BMDD) as well as osteocyte lacunar density. The BMDD of type V OI bone was distinctly shifted toward a higher degree of mineralization. The most frequently occurring calcium concentration (CaPeak) in cortical (Ct) and cancellous (Cn) bone was markedly increased (+11.5%, +10.4%, respectively, p < 0.0001) compared to healthy reference values. Treatment with pamidronate resulted in only a slight enhancement of mineralization. The osteocyte lacunar density derived from sectioned bone area was elevated in OI type V Ct and Cn bone (+171%, p < 0.0001; +183.3%, p < 0.01; respectively) versus controls. The high osteocyte density was associated with an overall immature primary bone structure (“mesh-like”) as visualized by polarized light microscopy. In summary, the bone material from OI type V patients is hypermineralized, similar to other forms of OI. The elevated osteocyte lacunar density in connection with lack of regular bone lamellation points to an exuberant primary bone formation and an alteration of the bone remodeling process in OI type V. © 2017 American Society for Bone and Mineral Research.
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The osteocyte network is dysregulated in patients with OI.
“This conclusion is supported by studies showing the osteocyte network is dysregulated in patients with OI72,73.”
From Osteocyte transcriptome mapping identifies a molecular landscape controlling skeletal homeostasis and susceptibility to skeletal disease (2021) · cites this paper as [72]
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Self-citation
- Authors
- Blouin, S., Fratzl-Zelman, N., Glorieux, F. H., Roschger, P., Klaushofer, K., Marini, J. C., & Rauch, F.
- Affiliations
- Hanusch Hospital (AT)
- Ludwig Boltzmann Institute of Osteology at Hanusch Hospital of WGKK and AUVA Trauma CentreMeidling, 1st Medical Department Hanusch HospitalViennaAustria
- Shriners Hospitals for Children - Canada (CA)
- National Institutes of Health (US)
- Corresponding author
- Blouin, Stéphane
- Venue
- Journal of Bone and Mineral Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-05-26
- Volume
- 32
- Issue
- 9
- Pages
- 1884-1892
- ISSN
- 0884-0431 (ISSN-L)
- 1523-4681
- PMC
- PMC5555797
- OpenAlex
- W2618375992
- Cited by
- 69
- References
- 52
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 15 · 2020: 11 · 2019: 8 · 2018: 5 · 2017: 2
- FWCI
- 4.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Bone and Mineral Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Allgemeine Unfallversicherungsanstalt
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Connective tissue disorders research
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Dermatological and Skeletal Disorders
- Keywords
- osteogenesis imperfecta type V, IFITM5, BRIL, hypermineralization, pamidronate, bisphosphonate therapy, bone remodeling, cortical and cancellous bone, pediatric patients, bone histomorphometry, interferon-inducible transmembrane proteins
- MeSH terms
- Cortical Bone, Cancellous Bone, Pamidronate, Collagen Type I, alpha 1 Chain, Adolescent, Calcinosis, Child, Child, Preschool, Diphosphonates, Female, Humans, Male, Membrane Proteins, Osteocytes, Osteogenesis, Osteogenesis Imperfecta, Bone Density, Collagen Type I
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