Stimulation by serum of multiplication of stationary chicken cells
Abstract
When serum was added to cultures of stationary chicken fibroblasts, there was an increase in the percentage of cells synthesizing DNA after a delay of about five hours. If the serum was removed before DNA synthesis began, some cells were still found to start DNA synthesis and to enter mitosis. The delay between the time of addition of serum and the start of DNA synthesis was not affected by the type of serum, the concentration of serum, nor the means of preparing the stationary cells. However, all of these factors affected the proportion of cells stimulated by serum. If two pulses of serum were given immediately following each other, their effects were synergistic. If there were twelve hours between them, there was no effect of the first pulse. These results were summarized in a new model of the cell cycle which subdivided the G1 phase into Gla, b, and c. The effects of serum were seen in a balanced salt solution, and were sensitive to several metabolic inhibitors.
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Suboptimal conditions such as high cell density or insufficient serum can drive cells into quiescence.
“Subsequent studies demonstrated that sub-optimal conditions such as high cell density7 or serum insufficiency8 could drive cells into this quiescent state.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [8]
Cites
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Self-citationTHE ROLE OF SERUM IN THE CONTROL OF MULTIPLICATION OF AVIAN AND MAMMALIAN CELLS IN CULTURE
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Self-citationTemin, H. M. (1969) · Journal of Cellular Physiology · doi:10.1002/jcp.1040740103 · Cited by 37 · see citing works
Self-citationControl of multiplication of uninfected rat cells and rat cells converted by murine sarcoma virus
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Self-citationLong G1 or G0 state: A method of resolving the dilemma for the cell cycle of an in vivo population
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G <sub>1</sub> Events and Regulation of Cell Proliferation
Pardee, A. B. (1989) · Science · doi:10.1126/science.2683075 · Cited by 2,182 · see citing works
Molecular regulation of stem cell quiescence
Cheung, T. H., & Rando, T. A. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3591 · Cited by 1,148 · see citing works
- Authors
- Temin, H. M.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Temin, Howard M.
- Venue
- Journal of Cellular Physiology
- Publisher
- Wiley
- Published
- 1971-10
- Volume
- 78
- Issue
- 2
- Pages
- 161-170
- ISSN
- 0021-9541 (ISSN-L)
- 1097-4652
- OpenAlex
- W2138728890
- Cited by
- 282
- References
- 11
- Citations by year
- 2023: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 4 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 14.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cellular Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- serum stimulation, chicken fibroblasts, DNA synthesis, cell cycle, G1 phase, mitosis, cell proliferation, synergistic effect, metabolic inhibitors, cell cycle model
- MeSH terms
- Animals, Blood, Cattle, Cells, Cultured, Chick Embryo, Chickens, Cycloheximide, Dactinomycin, DNA, DNA Replication, Fibroblasts, Mitosis, Models, Biological, Puromycin, Thymidine, Tritium
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