Polarized light imaging of white matter architecture
Abstract
Polarized light imaging (PLI) is a method to image fiber orientation in gross histological brain sections based on the birefringent properties of the myelin sheaths. The method uses the transmission of polarized light to quantitatively estimate the fiber orientation and inclination angles at every point of the imaged section. Multiple sections can be assembled into a 3D volume, from which the 3D extent of fiber tracts can be extracted. This article describes the physical principles of PLI and describes two major applications of the method: the imaging of white matter orientation of the rat brain and the generation of fiber orientation maps of the human brain in white and gray matter. The strengths and weaknesses of the method are set out. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
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The identified limitations of tractography will become even more pronounced in phantoms of higher anatomic complexity that might be achievable by involving independent methods such as polarized light imaging.
“It can be expected that the identified limitations of tractography will become even more pronounced in phantoms of higher anatomic complexity that might be achievable by involving independent methods such as polarized light imaging50.”
From Tractography-based connectomes are dominated by false-positive connections (2016) · cites this paper as [50]
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- Authors
- Larsen, L., Griffin, L. D., GRäßel, D., Witte, O. W., & Axer, H.
- Affiliations
- University College London (GB)
- Friedrich Schiller University Jena (DE)
- Corresponding author
- Axer, Hubertus
- Venue
- Microscopy Research and Technique
- Publisher
- Wiley
- Published
- 2007-07-27
- Volume
- 70
- Issue
- 10
- Pages
- 851-863
- ISSN
- 1059-910X (ISSN-L)
- 1097-0029
- OpenAlex
- W2062992566
- Cited by
- 96
- References
- 61
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 11 · 2020: 3 · 2019: 6 · 2018: 1 · 2017: 5 · 2016: 6 · 2015: 11 · 2014: 3 · 2013: 6 · 2012: 3
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microscopy Research and Technique (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Optical Polarization and Ellipsometry
- Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
- Keywords
- polarization imaging, white matter architecture, fiber orientation, rat brain, human brain, gray matter
- MeSH terms
- Animals, Birefringence, Brain, Humans, Image Enhancement, Image Processing, Computer-Assisted, Microscopy, Polarization, Nerve Fibers, Imaging, Three-Dimensional, Rats
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