Cited by 96Advanced Neuroimaging Techniques and ApplicationsOptical Polarization and EllipsometryMedical and Biological Sciences

Polarized light imaging of white matter architecture

Larsen, Luiza, Griffin, Lewis D., GRäßel, David, Witte, Otto W., Axer, Hubertus·2007·Microscopy Research and Technique

Abstract

Polarized light imaging (PLI) is a method to image fiber orientation in gross histological brain sections based on the birefringent properties of the myelin sheaths. The method uses the transmission of polarized light to quantitatively estimate the fiber orientation and inclination angles at every point of the imaged section. Multiple sections can be assembled into a 3D volume, from which the 3D extent of fiber tracts can be extracted. This article describes the physical principles of PLI and describes two major applications of the method: the imaging of white matter orientation of the rat brain and the generation of fiber orientation maps of the human brain in white and gray matter. The strengths and weaknesses of the method are set out. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

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Crossref · 10.1002/jemt.20488

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  • The identified limitations of tractography will become even more pronounced in phantoms of higher anatomic complexity that might be achievable by involving independent methods such as polarized light imaging.

    “It can be expected that the identified limitations of tractography will become even more pronounced in phantoms of higher anatomic complexity that might be achievable by involving independent methods such as polarized light imaging50.”

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Authors
Larsen, L., Griffin, L. D., GRäßel, D., Witte, O. W., & Axer, H.
Affiliations
  • University College London (GB)
  • Friedrich Schiller University Jena (DE)
Corresponding author
Axer, Hubertus
Venue
Microscopy Research and Technique
Publisher
Wiley
Published
2007-07-27
Volume
70
Issue
10
Pages
851-863
ISSN
  • 1059-910X (ISSN-L)
  • 1097-0029
OpenAlex
W2062992566
Cited by
96
References
61
Citations by year
2026: 5 · 2025: 8 · 2024: 5 · 2023: 7 · 2022: 4 · 2021: 11 · 2020: 3 · 2019: 6 · 2018: 1 · 2017: 5 · 2016: 6 · 2015: 11 · 2014: 3 · 2013: 6 · 2012: 3
FWCI
1.25
Type
journal article
Language
en
Open access
closed
Locations
  • Microscopy Research and Technique (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
  • Physical Sciences › Engineering › Biomedical Engineering › Optical Polarization and Ellipsometry
  • Health Sciences › Medicine › Anatomy › Medical and Biological Sciences
Keywords
polarization imaging, white matter architecture, fiber orientation, rat brain, human brain, gray matter
MeSH terms
Animals, Birefringence, Brain, Humans, Image Enhancement, Image Processing, Computer-Assisted, Microscopy, Polarization, Nerve Fibers, Imaging, Three-Dimensional, Rats

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