The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A triple‐stain technique for evaluating normal acrosome reactions of human sperm
Abstract
A triple‐stain technique has been developed to score normal acrosome‐reacted human sperm in fixed smears. Live and dead sperm are first differentiated using the vital stain trypan blue. Sperm are then fixed in glutar‐aldehyde, dried onto slides, and the postacrosomal region and acrosome are differentiated using Bismark brown and Rose Bengal. Slides are examined at 1,000 × with a bright‐field microscope and assessed for (1) the percentage of sperm that were alive at the time of fixation and (2) the percentage of sperm that had undergone normal acrosome reactions. Experiments are included that show that trypan blue is a reliable stain for dead sperm and that Rose Bengal stains only sperm having intact acrosomes. This technique may have applications in experimental and clinical studies on sperm capacitation, acrosome reactions, and fertilization in laboratory and domestic animals as well as in man.
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Cites
Biometry: The Principles and Practice of Statistics in Biological Research
Sokal, R. R., & Rohlf, F. J. (1969) · Cited by 20,963
Biometry. The Principles and Practice of Statistics in Biological Research
Fienberg, S. E., Sokal, R. R., Rohlf, F. J., Rohlf, F. J., & Sokal, R. R. (1970) · Biometrics · doi:10.2307/2529087 · Cited by 9,364
Dye Exclusion Tests for Cell Viability
Phillips, H. J. (1973) · Tissue Culture · doi:10.1016/b978-0-12-427150-0.50101-7 · Cited by 886
Cited by
Graham, J. K., Kunze, E., & Hammerstedt, R. H. (1990) · Biology of Reproduction · doi:10.1095/biolreprod43.1.55 · Cited by 324
Yanagimachi, R. (1984) · Gamete Research · doi:10.1002/mrd.1120100210 · Cited by 268
Saez Lancellotti, T. E., Boarelli, P. V., Romero, A. A., Funes, A. K., Cid-Barria, M., Cabrillana, M. E., Monclus, M. A., Simón, L., Vicenti, A. E., & Fornés, M. W. (2013) · PLoS ONE · doi:10.1371/journal.pone.0052386 · Cited by 57
Metadata
- Authors
- Talbot, P., & Chacon, R. S.
- Venue
- Journal of Experimental Zoology
- Publisher
- Wiley
- Published
- 1981-02
- Volume
- 215
- Issue
- 2
- Pages
- 201-208
- ISSN
- 0022-104X (ISSN-L)
- 1097-010X
- OpenAlex
- W1980066996
- Cited by
- 462
- References
- 3
- FWCI
- 5.00
- Type
- journal article
- Language
- en
- Open access
- closed
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- Talbot, P., & Chacon, R. S. (1981). A triple‐stain technique for evaluating normal acrosome reactions of human sperm. Journal of Experimental Zoology. https://doi.org/10.1002/jez.1402150210
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
