The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Analysis of Sperm Cell Viability, Acrosomal Integrity, and Mitochondrial Function Using Flow Cytometry1
Abstract
A triple staining procedure was developed to evaluate bull spermatozoa using flow cytometry. Flow cytometric estimates of cell viability, measured by propidium iodide (PI) exclusion, and acrosomal integrity, measured by Pisum sativum agglutinin (PSA) binding acrosomal contents, were equivalent to estimates made by using standard laboratory assays. Mitochondrial function, measured by rhodamine 123 (R123) fluorescence, was depressed by the mitochondrial inhibitors rotenone (64%) or monensin (52%), establishing that mitochondrial damage can be detected. Dilauroylphosphatidylcholine (PC12) or lysophosphatidylcholine (LPC) was used to destabilize sperm membranes. When challenged with 15-30 microM PC12, selective exposure of PSA binding sites occurred without induction of PI uptake or loss of R123 staining. However, PC12 concentrations greater than 60 microM resulted in a loss of R123 fluorescence intensity. In contrast, greater than 1200 microM LPC was required to expose PSA binding sites, which also resulted in PI uptake. By using flow cytometry, these three stains in combination can be used to correlate three different features simultaneously on individual spermatozoa and assay thousands of cells per sample without extensive preparation.
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Cites
Hope, M. J., Bally, M. B., Webb, G., & Cullis, P. R. (1985) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(85)90521-8 · Cited by 2,076
Capacitation of Bovine Sperm by Heparin1
Parrish, J. J., Parrish, J. J., Winer, M. A., & First, N. L. (1988) · Biology of Reproduction · doi:10.1095/biolreprod38.5.1171 · Cited by 1,583
Localization of mitochondria in living cells with rhodamine 123.
Johnson, L. V., Walsh, M. L., & Chen, L. B. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.2.990 · Cited by 1,553
Relation of Mammalian Sperm Chromatin Heterogeneity to Fertility
Evenson, D. P., Darzynkiewicz, Z., & Melamed, M. R. (1980) · Science · doi:10.1126/science.7444440 · Cited by 812
Two simple methods for detecting acrosome‐reacted human sperm
Cross, N. L., Morales, P., Overstreet, J. W., & Hanson, F. W. (1986) · Gamete Research · doi:10.1002/mrd.1120150303 · Cited by 601
A triple‐stain technique for evaluating normal acrosome reactions of human sperm
Talbot, P., & Chacon, R. S. (1981) · Journal of Experimental Zoology · doi:10.1002/jez.1402150210 · Cited by 462
Evenson, D. P., Darzynkiewicz, Z., & Melamed, M. R. (1982) · Journal of Histochemistry & Cytochemistry · doi:10.1177/30.3.6174566 · Cited by 240
Heterogeneity of Sperm Nuclear Chromatin Structure and its Relationship to Bull Fertility1
Ballachey, B. E., Hohenboken, W. D., & Evenson, D. P. (1987) · Biology of Reproduction · doi:10.1095/biolreprod36.4.915 · Cited by 210
BALLACHEY, B. E., EVENSON, D. P., & SAACKE, R. G. (1988) · Journal of Andrology · doi:10.1002/j.1939-4640.1988.tb01020.x · Cited by 208
Probing Mitochondria in Living Cells with Rhodamine 123
Chen, L. B., Summerhayes, I. C., Johnson, L. V., Walsh, M. L., Bernal, S. D., & Lampidis, T. J. (1982) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1982.046.01.018 · Cited by 184
Cited by
Viability Assessment of Mammalian Sperm Using SYBR-14 and Propidium Iodide1
Garner, D. L., & Johnson, L. A. (1995) · Biology of Reproduction · doi:10.1095/biolreprod53.2.276 · Cited by 717
Cellular Cargo Delivery: Toward Assisted Fertilization by Sperm-Carrying Micromotors
Medina-Sánchez, M., Schwarz, L., Meyer, A. K., Hebenstreit, F., & Schmidt, O. G. (2016) · Nano Letters · doi:10.1021/acs.nanolett.5b04221 · Cited by 553
Molecular mechanisms of calcium-dependent excitotoxicity
Sattler, R., & Tymianski, M. (2000) · Journal of Molecular Medicine · doi:10.1007/s001090000077 · Cited by 477
Metadata
- Authors
- Graham, J. K., Kunze, E., & Hammerstedt, R. H.
- Venue
- Biology of Reproduction
- Publisher
- Oxford University Press (OUP)
- Published
- 1990-07-01
- Volume
- 43
- Issue
- 1
- Pages
- 55-64
- ISSN
- 0006-3363 (ISSN-L)
- 1529-7268
- OpenAlex
- W2111735005
- Cited by
- 324
- References
- 12
- FWCI
- 8.29
- Type
- journal article
- Language
- en
- Open access
- closed
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- Graham, J. K., Kunze, E., & Hammerstedt, R. H. (1990). Analysis of Sperm Cell Viability, Acrosomal Integrity, and Mitochondrial Function Using Flow Cytometry1. Biology of Reproduction. https://doi.org/10.1095/biolreprod43.1.55
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
