The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Direct imaging of single cells and tissue at sub‐cellular spatial resolution using transmission geometry MALDI MS
Abstract
The need of cellular and sub‐cellular spatial resolution in laser desorption ionization (LDI)/matrix‐assisted LDI (MALDI) imaging mass spectrometry (IMS) necessitates micron and sub‐micron laser spot sizes at biologically relevant sensitivities, introducing significant challenges for MS technology. To this end, we have developed a transmission geometry vacuum ion source that allows the laser beam to irradiate the back side of the sample. This arrangement obviates the mechanical/ion optic complications in the source by completely separating the optical lens and ion optic structures. We have experimentally demonstrated the viability of transmission geometry MALDI MS for imaging biological tissues and cells with sub‐cellular spatial resolution. Furthermore, we demonstrate that in conjunction with new sample preparation protocols, the sensitivity of this instrument is sufficient to obtain molecular images at sub‐micron spatial resolution. Copyright © 2012 John Wiley & Sons, Ltd.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
Caprioli, R. M., Farmer, T. B., & Gile, J. (1997) · Analytical Chemistry · doi:10.1021/ac970888i · Cited by 2,234
Self-citation
Cited by
Norris, J. L., & Caprioli, R. M. (2013) · Chemical Reviews · doi:10.1021/cr3004295 · Cited by 696
Self-citationKompauer, M., Heiles, S., & Spengler, B. (2017) · Nature Methods · doi:10.1038/nmeth.4071 · Cited by 600
Transmission-mode MALDI-2 mass spectrometry imaging of cells and tissues at subcellular resolution
Niehaus, M., Soltwisch, J., Belov, M. E., & Dreisewerd, K. (2019) · Nature Methods · doi:10.1038/s41592-019-0536-2 · Cited by 429
Mass Spectrometry Imaging of Biomolecular Information
Spengler, B. (2015) · Analytical Chemistry · doi:10.1021/ac504543v · Cited by 290
MALDI imaging mass spectrometry: Spatial molecular analysis to enable a new age of discovery
Gessel, M. M., Norris, J. L., & Caprioli, R. M. (2014) · Journal of Proteomics · doi:10.1016/j.jprot.2014.03.021 · Cited by 276
Self-citation
Metadata
- Authors
- Zavalin, A., Todd, E. M., Rawhouser, P. D., Yang, J., Norris, J. L., & Caprioli, R. M.
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 2012-11
- Volume
- 47
- Issue
- 11
- Pages
- 1473-1481
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W2313300359
- Cited by
- 236
- References
- 2
- FWCI
- 8.91
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Direct imaging of single cells and tissue at sub‐cellular spatial resolution using transmission geometry MALDI MS. https://app.cochat.ai/papers/10.1002/jms.3108
- The paper
- Zavalin, A., Todd, E. M., Rawhouser, P. D., Yang, J., Norris, J. L., & Caprioli, R. M. (2012). Direct imaging of single cells and tissue at sub‐cellular spatial resolution using transmission geometry MALDI MS. Journal of Mass Spectrometry. https://doi.org/10.1002/jms.3108
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
