An analytical method for the simultaneous quantification of 30 bioactive compounds in spent coffee ground by HPLC‐MS/MS
Abstract
Spent coffee ground (SCG) is the remaining residue produced after extraction of coffee, and it is considered a source of unextracted bioactive compounds. For this, in the latest years, the attention has been focused to innovative reuses that can exploit the potentiality of SCG. Unfortunately, the content of bioactive compounds has not been thoroughly studied yet, and the major of publication has investigated the caffeine and chlorogenic acids levels, total polyphenol contents, and total flavonoid content. Hence, these approaches have determined only an estimation of flavonoids and polyphenols content and lack on single polyphenols investigation. Therefore, the objective of the current work was to provide a deep characterization of bioactive compounds in SCG. For this purpose, a new analytical method for the quantification of 30 molecules, including caffeine, chlorogenic acids, phenolic acids, flavonoids, and secoiridoids, has been developed using high‐performance liquid chromatography tandem mass spectrometry. Moreover, several extraction procedures, that is, liquid–solid extraction assisted and not by ultrasounds, testing diverse solvents, were evaluated. Liquid–solid extraction assisted by sonication, with water/ethanol (30/70, v/v), resulted the best in terms of total bioactive compounds, and, once validated, the new analytical method was applied to five different espresso SCG samples. Data showed that caffeine (means: 1193.886 ± 57.307 mg kg−1) and chlorogenic acids (means of total CQAs: 1705.656 ± 88.694 mg kg−1) were the most abundant compounds in all SCG samples followed by phenolic acids such as caffeic, ferulic, gallic, p‐coumaric, syringic, trans‐cinnamic, and vanillic acid. Moreover, some flavonoids, that is, rutin, cyanidin 3‐glucoside, and quercetin, occurred in almost all samples. This work provided a deepened characterization of bioactive compounds in SCG and can contribute to develop new strategies of reuses.
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Coumaric, gentisic, and ferulic acids were the lowest among the identified constituents in the methanolic SCG extract.
“On the other hand, coumaric (0.16 µg/g), gentisic (0.26 µg/g), and ferulic (0.37 µg/g) acids were the lowest compared to the identified constituents, which is in agreement with Angeloni et al. [22] (Table 1).”
From The Valorization of Spent Coffee Ground Extract as a Prospective Insecticidal Agent against Some Main Key Pests of Phaseolus vulgaris in the Laboratory and Field (2022) · cites this paper as Angeloni et al. [22]
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Spent coffee grounds: A review on current research and future prospects
Campos-Vega, R., Loarca-Piña, G., Vergara-Castañeda, H. A., & Oomah, B. D. (2015) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2015.04.012 · Cited by 679 · see citing works
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Zengin, G., Sinan, K. I., Mahomoodally, M. F., Angeloni, S., Mustafa, A. M., Vittori, S., Maggi, F., & Caprioli, G. (2020) · Foods · doi:10.3390/foods9060713 · Cited by 122 · see citing works
Self-citationAngeloni, S., Freschi, M., Marrazzo, P., Hrelia, S., Beghelli, D., Juan-García, A., Juan, C., Caprioli, G., Sagratini, G., & Angeloni, C. (2021) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2021/6620913 · Cited by 50 · see citing works
Hussein, H., Abouamer, W., Ali, H., Elkhadragy, M., Yehia, H., & Farouk, A. (2022) · Plants · doi:10.3390/plants11091124 · Cited by 20 · see citing works
- Authors
- Angeloni, S., Nzekoue, F. K., Navarini, L., Sagratini, G., Torregiani, E., Vittori, S., & Caprioli, G.
- Affiliations
- Università di Camerino (IT)
- illycaffè S.p.A. Trieste Italy
- Corresponding author
- Caprioli, Giovanni
- ORCID
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 2020-05-05
- Volume
- 55
- Issue
- 11
- Pages
- e4519-e4519
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W3014889254
- Cited by
- 62
- References
- 1
- Citations by year
- 2026: 8 · 2025: 14 · 2024: 13 · 2023: 8 · 2022: 4 · 2021: 13 · 2020: 2
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Mass Spectrometry (publishedVersion)
- PubMed (publishedVersion)
- Unicam Scientific Publications (University of Camerino) (submittedVersion)
- Funders
- Università degli Studi di Camerino (FPI000051)
- Topics
- Health Sciences › Medicine › Pharmacology › Coffee research and impacts
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- spent coffee grounds, bioactive compounds, caffeine, chlorogenic acid, phenolic acids, flavonoids, secoiridoids, ultrasound-assisted extraction, HPLC-MS/MS quantification
- MeSH terms
- Caffeine, Chlorogenic Acid, Chromatography, High Pressure Liquid, Coffee, Flavonoids, Phenols, Iridoids, Coffea, Tandem Mass Spectrometry, Polyphenols
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