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Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation

Open access
Schwartz, Sarah A., Reyzer, Michelle L., Caprioli, Richard M.·2003·Journal of Mass Spectrometry

Abstract

Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.

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Metadata

Authors
Schwartz, S. A., Reyzer, M. L., & Caprioli, R. M.
Venue
Journal of Mass Spectrometry
Publisher
Wiley
Published
2003-07
Volume
38
Issue
7
Pages
699-708
ISSN
  • 1076-5174 (ISSN-L)
  • 1096-9888
OpenAlex
W2089706020
Cited by
646
References
1
FWCI
11.51
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. https://app.cochat.ai/papers/10.1002/jms.505
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Schwartz, S. A., Reyzer, M. L., & Caprioli, R. M. (2003). Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. Journal of Mass Spectrometry. https://doi.org/10.1002/jms.505

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.