The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Improved diagnosis on a daily basis of enterovirus meningitis using a one‐step real‐time RT‐PCR assay
Abstract
The detection of the enterovirus genome in cerebrospinal fluid (CSF) by PCR techniques has proved to be more sensitive than traditional cell culture for the diagnosis of enterovirus meningitis. However, PCR assays are time consuming and labor intensive, particularly if separate hybridization steps are used to confirm the specificity of positive findings. The aim of this study was to develop a one‐step real‐time RT‐PCR assay with LightCycler (LC) technology that was sensitive, rapid, and easy to perform in routine practice. The enterovirus detection limit was determined by testing 10‐fold limiting dilution series of cell culture stocks with the echovirus 25 (E‐25) prototype strain and with the third European Union Quality Control Concerted Action (EU‐QCCA) enterovirus proficiency panel. A total of 100 CSF specimens were investigated in a comparative study. With the E‐25 strain, the detection limit of the real‐time assay was 286 TCID 50/ml (50% tissue culture infective dose). When samples of the EU‐QCCA panel were tested, our assay gave identical results (detection limit down to 3.6 TCID 50/ml) to those of the reference laboratory, which used one‐step RT‐PCR assay. When CSF specimens were tested, there was a correlation between the real‐time assay and the conventional in‐house assay in 96 of 100 CSFs tested. This one‐step real‐time assay allows rapid enterovirus detection in CSF since results are obtained in 3 hr as against 36 hr with the “in‐house” RT‐PCR assay. This new assay is now being used in routine practice, and allows diagnosis on a daily basis. J. Med. Virol. 74:604–611, 2004. © 2004 Wiley‐Liss, Inc.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The reason that only Enteroviridae were detected in this study and that no etiologic agents were identified in two-thirds of cases is likely that cultures on CSF are not sensitive for other viruses [13].”
From Paediatric Investigators Collaborative Network on Infections in Canada (PICNIC) study of aseptic meningitis (2006) · cites this paper as [13]
Citations
Cites
The LightCycler TM : A Microvolume Multisample Fluorimeter with Rapid Temperature Control
Wittwer, C. T., Ririe, K. M., Andrew, R. V., David, D. A., Gundry, R. A., & Balis, U. J. (1997) · BioTechniques · doi:10.2144/97221pf02 · Cited by 893
Treatment of Potentially Life-Threatening Enterovirus Infections with Pleconaril
Rotbart, H. A., Webster, A. D., & for the Pleconaril Treatment Registry Group (2001) · Clinical Infectious Diseases · doi:10.1086/318452 · Cited by 373
Enteroviral infections of the central nervous system.
Rotbart, H. A. (1995) · Clinical Infectious Diseases · doi:10.1093/clinids/20.4.971 · Cited by 312
Enzymatic RNA amplification of the enteroviruses
Rotbart, H. A. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.3.438-442.1990 · Cited by 292
Casas, I., Powell, L., Klapper, P. E., & Cleator, G. M. (1995) · Journal of Virological Methods · doi:10.1016/0166-0934(94)00173-e · Cited by 229
Lim, K. A., & Benyésh-Melnick, M. (1960) · The Journal of Immunology · doi:10.4049/jimmunol.84.3.309 · Cited by 210
Nijhuis, M., van Maarseveen, N. M., Schuurman, R., Verkuijlen, S. A. W. M., de Vos, M., Hendriksen, K. A. W., & van Loon, A. M. (2002) · Journal of Clinical Microbiology · doi:10.1128/jcm.40.10.3666-3670.2002 · Cited by 164
Quantification of Enterovirus RNA in Sludge Samples Using Single Tube Real-Time RT-PCR
Monpoeho, S., Dehée, A., Mignotte, B., Schwartzbrod, L., Maréchal, V., Nicolas, J., Billaudel, S., & Ferré, V. (2000) · BioTechniques · doi:10.2144/00291st03 · Cited by 142
Chandler, D. P., Wagnon, C. A., & Bolton, H. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.2.669-677.1998 · Cited by 141
Reverse-Transcription Polymerase Chain Reaction Detection of the Enteroviruses
Romero, J. (1999) · Archives of Pathology & Laboratory Medicine · doi:10.5858/1999-123-1161-rtpcrd · Cited by 137
Cited by
Detection and monitoring of virus infections by real-time PCR
Watzinger, F., Ebner, K., & Lion, T. (2006) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2005.12.001 · Cited by 281
Chadwick, D. (2005) · British Medical Bulletin · doi:10.1093/bmb/ldh057 · Cited by 108
Archimbaud, C., Chambon, M., Bailly, J., Petit, I., Henquell, C., Mirand, A., Aublet‐Cuvelier, B., Ughetto, S., Beytout, J., Clavelou, P., et al. (2008) · Journal of Medical Virology · doi:10.1002/jmv.21330 · Cited by 108
Self-citationMirand, A., Henquell, C., Archimbaud, C., Chambon, M., Charbonné, F., Peigue‐Lafeuille, H., & Bailly, J. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.01020-07 · Cited by 98
Self-citationPerformance of a predictive rule to distinguish bacterial and viral meningitis
Chavanet, P., Schaller, C., Levy, C., Flores-Cordero, J. M., Arens, M. Q., Piroth, L., Bingen, É., & Portier, H. (2006) · Journal of Infection · doi:10.1016/j.jinf.2006.06.009 · Cited by 65
Metadata
- Authors
- Archimbaud, C., Mirand, A., Chambon, M., Regagnon, C., Bailly, J., Peigue‐Lafeuille, H., & Henquell, C.
- Affiliations
- Centre Hospitalier Universitaire de Clermont-Ferrand (FR)
- Corresponding author
- Archimbaud, Christine
- Venue
- Journal of Medical Virology
- Publisher
- Wiley
- Published
- 2004-10-13
- Volume
- 74
- Issue
- 4
- Pages
- 604-611
- ISSN
- 0146-6615 (ISSN-L)
- 1096-9071
- OpenAlex
- W2125092867
- Cited by
- 28
- References
- 26
- Citations by year
- 2024: 1 · 2023: 1 · 2021: 1 · 2018: 1 · 2017: 1 · 2013: 5 · 2012: 1
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Medical Virology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Immunology Research
- Health Sciences › Medicine › Infectious Diseases › Infectious Encephalopathies and Encephalitis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- enterovirus meningitis, enterovirus detection, cerebrospinal fluid, LightCycler, detection limit, rapid diagnosis
- MeSH terms
- Cerebrospinal Fluid, Enterovirus Infections, Enterovirus, Humans, Meningitis, Viral, RNA, Viral, Sensitivity and Specificity, Time Factors, Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Improved diagnosis on a daily basis of enterovirus meningitis using a one‐step real‐time RT‐PCR assay. https://app.cochat.ai/papers/10.1002/jmv.20217
- The paper
- Archimbaud, C., Mirand, A., Chambon, M., Regagnon, C., Bailly, J., Peigue‐Lafeuille, H., & Henquell, C. (2004). Improved diagnosis on a daily basis of enterovirus meningitis using a one‐step real‐time RT‐PCR assay. Journal of Medical Virology. https://doi.org/10.1002/jmv.20217
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
