Effects of age and platelet-rich plasma on ACL cell viability and collagen gene expression

Cheng, Mingyu; Johnson, Victor M.; Murray, Martha M.

doi:10.1002/jor.21496

Cited by 40Periodontal Regeneration and TreatmentsTendon Structure and TreatmentKnee injuries and reconstruction techniques

Effects of age and platelet-rich plasma on ACL cell viability and collagen gene expression

Open access

Cheng, Mingyu, Johnson, Victor M., Murray, Martha M.·2012·Journal of Orthopaedic Research

DOI 10.1002/jor.21496PMC PMC3202019

Open this paper in CoChat — free account Open PDF

Abstract

Platelet‐rich plasma (PRP) has shown in vivo potential to stimulate anterior cruciate ligament (ACL) healing at early time points in large animal models. However, in animal models, the healing potential of the ACL is dependent on animal age. In this study, we hypothesized that there are age‐dependent differences in ACL cell metabolism, collagen gene expression, and the ability of the cells to respond to growth factors in PRP. To test this hypothesis, ACL cells were obtained from skeletally immature, adolescent and adult pigs, and cultured in a collagen type I hydrogel with or without PRP for 14 days. When cultured in collagen‐only hydrogel, ACL cells from adult pigs had a 19% lower apoptotic rate as compared to immature pigs (p = 0.001) and a 25% higher cellular metabolic activity as compared to adolescent pigs (p = 0.006). The addition of PRP to the collagen hydrogel resulted in a significantly increased cellular metabolic activity, reduced apoptotic rate, and stimulation of collagen production in the cells from the immature and adolescent animals (p < 0.05 for all comparisons) but had less effect on adult cells. These findings suggest that skeletal maturity may influence ACL cells' metabolic activity, apoptosis, collagen production, and response to PRP. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:79–85, 2012

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1002/jor.21496

Cites

37 of 37 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

In ACL cells from immature and adolescent pigs, PRP produced higher cellular metabolic activity and reduced apoptotic rate.
“Higher cellular metabolic activity and reduced apoptotic rate were found in immature and adolescent animals after the administration of PRP.”
From Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review (2023) · cites this paper as [33]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Platelet-Rich Plasma (PRP): What Is PRP and What Is Not PRP?
Marx, R. E. (2001) · Implant Dentistry · doi:10.1097/00008505-200110000-00002 · Cited by 1,709 · see citing works
The Cell and Molecular Biology of Fracture Healing
Einhorn, T. A. (1998) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-199810001-00003 · Cited by 1,280 · see citing works
Age-related changes in human bone marrow-derived mesenchymal stem cells: Consequences for cell therapies
Stolzing, A., Jones, E., McGonagle, D., & Scutt, A. (2008) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2007.12.002 · Cited by 1,195 · see citing works
High prevalence of osteoarthritis 14 years after an anterior cruciate ligament tear in male soccer players: a study of radiographic and patient relevant outcomes
von Porat, A., Roos, E. M., & Roos, H. P. (2004) · Annals of the Rheumatic Diseases · doi:10.1136/ard.2003.008136 · Cited by 850 · see citing works
Knee Function and Prevalence of Knee Osteoarthritis after Anterior Cruciate Ligament Reconstruction
Øiestad, B. E., Holm, I., Aune, A. K., Gunderson, R., Myklebust, G., Engebretsen, L., Fosdahl, M. A., & Risberg, M. A. (2010) · The American Journal of Sports Medicine · doi:10.1177/0363546510373876 · Cited by 434 · see citing works
Histological Changes in the Human Anterior Cruciate Ligament After Rupture*
MURRAY, M. M., MARTIN, S. D., MARTIN, T. L., & SPECTOR, M. (2000) · The Journal of Bone and Joint Surgery-American Volume · doi:10.2106/00004623-200010000-00004 · Cited by 398 · see citing works
Self-citation
Controlled delivery of PDGF-BB for myocardial protection using injectable self-assembling peptide nanofibers
Hsieh, P. C. H. (2005) · Journal of Clinical Investigation · doi:10.1172/jci25878 · Cited by 357 · see citing works
Morphological and Molecular Characterization of Adult Cardiomyocyte Apoptosis During Hypoxia and Reoxygenation
Kang, P. M., Haunstetter, A., Aoki, H., Usheva, A., & Izumo, S. (2000) · Circulation Research · doi:10.1161/01.res.87.2.118 · Cited by 323 · see citing works
Collagen-platelet rich plasma hydrogel enhances primary repair of the porcine anterior cruciate ligament
Murray, M. M., Spindler, K. P., Abreu, E., Muller, J. A., Nedder, A., Kelly, M., Frino, J., Zurakowski, D., Valenza, M., Snyder, B. D., et al. (2007) · Journal of Orthopaedic Research · doi:10.1002/jor.20282 · Cited by 321 · see citing works
Self-citation
Insulin-like growth factor-I receptor. Its role in cell proliferation, apoptosis, and tumorigenicity.
Rubin, R., & Baserga, R. (1995) · PubMed · Cited by 311 · see citing works
Enhanced histologic repair in a central wound in the anterior cruciate ligament with a collagen–platelet‐rich plasma scaffold
Murray, M. M., Spindler, K. P., Ballard, P., Welch, T. P., Zurakowski, D., & Nanney, L. B. (2007) · Journal of Orthopaedic Research · doi:10.1002/jor.20367 · Cited by 300 · see citing works
Self-citation
The Effect of TGF-β on Keloid Fibroblast Proliferation and Collagen Synthesis
Bettinger, D. A., Yager, D. R., Diegelmann, R. F., & Cohen, K. I. (1996) · Plastic & Reconstructive Surgery · doi:10.1097/00006534-199610000-00012 · Cited by 294 · see citing works
Mesenchymal stem cells derived from synovium, meniscus, anterior cruciate ligament, and articular chondrocytes share similar gene expression profiles
Segawa, Y., Muneta, T., Makino, H., Nimura, A., Mochizuki, T., Ju, Y., Ezura, Y., Umezawa, A., & Sekiya, I. (2008) · Journal of Orthopaedic Research® · doi:10.1002/jor.20786 · Cited by 206 · see citing works
Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL
Murray, M. M., Spindler, K. P., Devin, C., Snyder, B. S., Muller, J., Takahashi, M., Ballard, P., Nanney, L. B., & Zurakowski, D. (2006) · Journal of Orthopaedic Research · doi:10.1002/jor.20073 · Cited by 193 · see citing works
Self-citation
Activation of Platelet-Rich Plasma Using Soluble Type I Collagen
Fufa, D. T., Shealy, B., Jacobson, M. S., Kevy, S. V., & Murray, M. M. (2008) · Journal of Oral and Maxillofacial Surgery · doi:10.1016/j.joms.2007.06.635 · Cited by 180 · see citing works
Self-citation
Biologic Incorporation of Allograft Anterior Cruciate Ligament Replacements
Jackson, D. W., Corsetti, J. R., & Simon, T. M. (1996) · Clinical Orthopaedics and Related Research · doi:10.1097/00003086-199603000-00015 · Cited by 165 · see citing works
Long-term results of anterior cruciate ligament reconstruction: a comparison with non-operative treatment with a follow-up of 17–20 years
Mihelić, R., Jurdana, H., Jotanović, Z., Mađarević, T., & Tudor, A. (2011) · International Orthopaedics · doi:10.1007/s00264-011-1206-x · Cited by 163 · see citing works
The effect of selected growth factors on human anterior cruciate ligament cell interactions with a three‐dimensional collagen‐GAG scaffold
Murray, M. M., Rice, K., Wright, R., & Spector, M. (2003) · Journal of Orthopaedic Research® · doi:10.1016/s0736-0266(02)00142-0 · Cited by 132 · see citing works
Self-citation
Characterization of human serum spreading factor with monoclonal antibody.
Barnes, D. W., Silnutzer, J., See, C., & Shaffer, M. C. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.5.1362 · Cited by 124 · see citing works
Fibronectin induces cell proliferation and inhibits apoptosis in human bronchial epithelial cells: pro-oncogenic effects mediated by PI3-kinase and NF-κB
Han, S. W., & Roman, J. (2006) · Oncogene · doi:10.1038/sj.onc.1209460 · Cited by 115 · see citing works
Cyclin A/cdk2 Activation Is Involved in Hypoxia-Induced Apoptosis in Cardiomyocytes
Adachi, S., Ito, H., Tamamori‐Adachi, M., Ono, Y., Nozato, T., Abe, S., Ikeda, M., Marumo, F., & Hiroe, M. (2001) · Circulation Research · doi:10.1161/01.res.88.4.408 · Cited by 114 · see citing works
Mesenchymal Stem Cell Characteristics of Human Anterior Cruciate Ligament Outgrowth Cells
Steinert, A. F., Kunz, M., Prager, P., Barthel, T., Jakob, F. J., Nöth, U. A., Murray, M. M., Evans, C. H., & Porter, R. M. (2011) · Tissue Engineering Part A · doi:10.1089/ten.tea.2010.0413 · Cited by 107 · see citing works
Self-citation
Reconstructive versus non-reconstructive treatment of anterior cruciate ligament insufficiency. A retrospective matched-pair long-term follow-up
Streich, N. A., Zimmermann, D., Bode, G., & Schmitt, H. (2010) · International Orthopaedics · doi:10.1007/s00264-010-1174-6 · Cited by 101 · see citing works
The Effect of Skeletal Maturity on Functional Healing of the Anterior Cruciate Ligament
Murray, M. M., Magarian, E. M., Harrison, S. L., Mastrangelo, A. N., Zurakowski, D., & Fleming, B. C. (2010) · Journal of Bone and Joint Surgery · doi:10.2106/jbjs.i.01368 · Cited by 100 · see citing works
Self-citation
The natural history of the meniscus in anterior cruciate insufficiency. Arthroscopic analysis
Irvine, G., & Glasgow, M. M. S. (1992) · Journal of Bone and Joint Surgery - British Volume · doi:10.1302/0301-620x.74b3.1587888 · Cited by 98 · see citing works
A radioimmunoassay for thrombospondin, used in a comparative study of thrombospondin, β-thromboglobulin and platelet factor 4 in healthy volunteers
Dawes, J., Clemetson, K. J., Gogstad, G. O., McGregor, J. L., Clézardin, P., Prowse, C. V., & Pepper, D. S. (1983) · Thrombosis Research · doi:10.1016/0049-3848(83)90212-8 · Cited by 85 · see citing works
DISTRIBUTION OF COLD‐INSOLUBLE GLOBULIN IN PLASMA AND TISSUES*
Matsuda, M., YOSHIDA, N., Aoki, N., & Wakabayashi, K. (1978) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1978.tb16794.x · Cited by 74 · see citing works
Anatomic Double-bundle ACL Reconstruction
Schreiber, V. M., van Eck, C. F., & Fu, F. H. (2010) · Sports Medicine and Arthroscopy Review · doi:10.1097/jsa.0b013e3181bf6634 · Cited by 71 · see citing works
Immature animals have higher cellular density in the healing anterior cruciate ligament than adolescent or adult animals
Mastrangelo, A. N., Haus, B. M., Vavken, P., Palmer, M. P., Machan, J. T., & Murray, M. M. (2010) · Journal of Orthopaedic Research® · doi:10.1002/jor.21070 · Cited by 63 · see citing works
Self-citation
The effect of skeletal maturity on the regenerative function of intrinsic ACL cells
Mastrangelo, A. N., Magarian, E. M., Palmer, M. P., Vavken, P., & Murray, M. M. (2009) · Journal of Orthopaedic Research® · doi:10.1002/jor.21018 · Cited by 59 · see citing works
Self-citation
Age dependence of expression of growth factor receptors in porcine ACL fibroblasts
Vavken, P., Saad, F. A., & Murray, M. M. (2010) · Journal of Orthopaedic Research® · doi:10.1002/jor.21111 · Cited by 55 · see citing works
Self-citation
Biomechanical comparison of three anatomic ACL reconstructions in a porcine model
Anibal Debandi, L., Maeyama, A., Lü, S.-C., Hume, C., Asai, S., Goto, B., Hoshino, Y. Y., Smolinski, P. J., & Fu, F. H. (2010) · Knee Surgery Sports Traumatology Arthroscopy · doi:10.1007/s00167-010-1338-3 · Cited by 44 · see citing works
Human anterior cruciate ligament fibroblasts from immature patients have a stronger in vitro response to platelet concentrates than those from mature individuals
Magarian, E. M., Vavken, P., & Murray, M. M. (2010) · The Knee · doi:10.1016/j.knee.2010.05.015 · Cited by 35 · see citing works
Self-citation
Collagen density significantly affects the functional properties of an engineered provisional scaffold
de Abreu, E. L., Palmer, M. P., & Murray, M. M. (2009) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.32508 · Cited by 17 · see citing works
Self-citation
ACL Reconstruction with Double-Bundle Technique: A Review of Clinical Results
Järvelä, T., & Suomalainen, P. (2011) · The Physician and Sportsmedicine · doi:10.3810/psm.2011.02.1865 · Cited by 16 · see citing works
High prevalence of osteoarthritis 14 years after an anterior cruciate ligament tear in male soccer players: a study of radiographic and patient relevant outcomes
von Porat, A., Roos, E. M., & Roos, H. P. (2004) · PubMed Central · Cited by 15 · see citing works
Principle Considerations in Anatomic ACL Reconstruction
Hensler, D., Illingworth, K. D., & Fu, F. H. (2010) · Arthroscopy The Journal of Arthroscopic and Related Surgery · doi:10.1016/j.arthro.2010.08.020 · Cited by 10 · see citing works

Flagged first, then by citations

1 of the 38 references couldn’t be matched to a known paper.

Sorted by citations

Evaluation of the effects of platelet-rich plasma (PRP) therapy involved in the healing of sports-related soft tissue injuries.
Middleton, K. K., Barro, V. M., Muller, B., Terada, S., & Fu, F. H. (2012) · Europe PMC (PubMed Central) · Cited by 147 · see citing works
Rise of the Pigs: Utilization of the Porcine Model to Study Musculoskeletal Biomechanics and Tissue Engineering During Skeletal Growth
Cone, S. G., Warren, P. B., & Fisher, M. B. (2017) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2017.0227 · Cited by 105 · see citing works
PRP Augmentation for ACL Reconstruction
Andriolo, L., Matteo, B. D., Kon, E., Filardo, G., Venieri, G., & Marcacci, M. (2015) · BioMed Research International · doi:10.1155/2015/371746 · Cited by 87 · see citing works
The Mature Athlete
McCarthy, M. M., & Hannafin, J. A. (2013) · Sports Health A Multidisciplinary Approach · doi:10.1177/1941738113485691 · Cited by 85 · see citing works
Molecular and Biologic Effects of Platelet-Rich Plasma (PRP) in Ligament and Tendon Healing and Regeneration: A Systematic Review
Chalidis, B., Givissis, P., Papadopoulos, P., & Pitsilos, C. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032744 · Cited by 74 · see citing works
“In another study from the same authors [33], ACL cells from immature, adolescent and adult pigs were cultured in COL hydrogel with or without PRP and the correlation between age and metabolic stimulation of PRP was examined.”
Background
Biologic Approaches for the Treatment of Partial Tears of the Anterior Cruciate Ligament
Dallo, I., Chahla, J. A., Mitchell, J. J., Pascual‐Garrido, C., Feagin, J. A., & LaPrade, R. F. (2017) · Orthopaedic Journal of Sports Medicine · doi:10.1177/2325967116681724 · Cited by 65 · see citing works
Effects of Electrical Stimulation on Articular Cartilage Regeneration with a Focus on Piezoelectric Biomaterials for Articular Cartilage Tissue Repair and Engineering
Zhou, Z., Zheng, J., Meng, X., & Wang, F. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24031836 · Cited by 55 · see citing works
Platelet Rich Plasma and Knee Surgery
Sánchez, M., Delgado, D., Sánchez, P., Fiz, N., Azofra, J., Orive, G., Anitua, E., & Padilla, S. (2014) · BioMed Research International · doi:10.1155/2014/890630 · Cited by 48 · see citing works
Mechanisms of Bone Tunnel Enlargement Following Anterior Cruciate Ligament Reconstruction
Li, Y., DeFroda, S. F., Sullivan, K., Garcia, D. R., & Owens, B. D. (2020) · JBJS Reviews · doi:10.2106/jbjs.rvw.19.00120 · Cited by 46 · see citing works
Platelet-Rich Plasma and Bone Defect Healing
Malhotra, A., Pelletier, M. H., Oliver, R. A., Christou, C., & Walsh, W. R. (2014) · Tissue Engineering Part A · doi:10.1089/ten.tea.2013.0737 · Cited by 42 · see citing works
Platelet-rich plasma in orthopaedic sports medicine: state of the art
Milano, G., Sánchez, M., Jo, C. H., Saccomanno, M. F., Thampatty, B. P., & Wang, J. H. (2019) · Journal of ISAKOS Joint Disorders & Orthopaedic Sports Medicine · doi:10.1136/jisakos-2019-000274 · Cited by 39 · see citing works
Differential effect of platelet-rich plasma fractions on β1-integrin signaling, collagen biosynthesis, and prolidase activity in human skin fibroblasts
Guszczyn, T., Surażyński, A., Zaręba, I., Rysiak, E., Popko, J., & Palka, J. A. (2017) · Drug Design Development and Therapy · doi:10.2147/dddt.s135949 · Cited by 30 · see citing works
Ortho-Biologics for Ligament Repair and Reconstruction
Chahla, J. A., Kennedy, M. I., Aman, Z. S., & LaPrade, R. F. (2018) · Clinics in Sports Medicine · doi:10.1016/j.csm.2018.08.003 · Cited by 29 · see citing works
Management of Anterior Cruciate Ligament Injuries in Adults Aged >40 Years
Salzler, M. J., Chang, J., & Richmond, J. C. (2018) · Journal of the American Academy of Orthopaedic Surgeons · doi:10.5435/jaaos-d-16-00730 · Cited by 28 · see citing works
The Efficacy of Platelet-Rich Plasma for Ligament Injuries: A Systematic Review of Basic Science Literature With Protocol Quality Assessment
Kunze, K. N., Pakanati, J. J., Vadhera, A. S., Polce, E. M., Williams, B. T., Parvaresh, K. C., & Chahla, J. A. (2022) · Orthopaedic Journal of Sports Medicine · doi:10.1177/23259671211066504 · Cited by 28 · see citing works
Aged Mice Repeatedly Injected with Plasma from Young Mice: A Survival Study
Shytikov, D. W., Balva, O., Debonneuil, E. H., Glukhovskiy, P., & Pishel, I. (2014) · BioResearch open access · doi:10.1089/biores.2014.0043 · Cited by 24 · see citing works
Use of a platelet-rich plasma-collagen scaffold as a bioenhanced repair treatment for management of partial cruciate ligament rupture in dogs
Sample, S. J., Racette, M. A., Hans, E. C., Volstad, N. J., Schaefer, S. L., Bleedorn, J. A., Little, J. P., Waller, K. R., Hao, Z., Block, W. F., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0197204 · Cited by 27 · see citing works
Current hip cartilage regeneration/repair modalities: a scoping review of biologics and surgery
Hevesi, M., Jacob, G., Shimomura, K., Ando, W., Nakamura, N., & Krych, A. J. (2020) · International Orthopaedics · doi:10.1007/s00264-020-04789-2 · Cited by 26 · see citing works
Tissue engineering approaches for the repair and regeneration of the anterior cruciate ligament: towards 3D bioprinted ACL-on-chip
Bakırcı, E., Guenat, O. T., Ahmad, S. S., & Gantenbein, B. (2022) · European Cells and Materials · doi:10.22203/ecm.v044a02 · Cited by 26 · see citing works
Mid-term outcomes of anterior cruciate ligament reconstruction across age groups: A national database study
Kelly, M. E., Turcotte, J. J., Thomas, D. M., Petre, B. M., Morganti, C. M., York, J. J., & Redziniak, D. E. (2021) · Journal of Orthopaedics · doi:10.1016/j.jor.2021.01.003 · Cited by 21 · see citing works
Functional Ultra-High Molecular Weight Polyethylene Composites for Ligament Reconstructions and Their Targeted Applications in the Restoration of the Anterior Cruciate Ligament
Wahed, S. B., Dunstan, C. R., Boughton, P. C., Ruys, A. J., Faisal, S. N., Wahed, T. B., Salahuddin, B., Cheng, X., Zhou, Y., Wang, C., et al. (2022) · Polymers · doi:10.3390/polym14112189 · Cited by 21 · see citing works
Biological Augmentation of ACL Repair and Reconstruction: Current Status and Future Perspective
Uchida, R., Jacob, G., Shimomura, K., Horibe, S., & Nakamura, N. (2020) · Sports Medicine and Arthroscopy Review · doi:10.1097/jsa.0000000000000266 · Cited by 21 · see citing works
Primary Anterior Cruciate Ligament Repair With Hyaluronic Scaffold and Autogenous Bone Marrow Aspirate Augmentation in Adolescents With Open Physes
Gobbi, A. W., Herman, K., Grabowski, R., & Szwedowski, D. (2019) · Arthroscopy Techniques · doi:10.1016/j.eats.2019.08.016 · Cited by 17 · see citing works
Nonoperative and Operative Bone and Cartilage Regeneration and Orthopaedic Biologics of the Hip: An Orthoregeneration Network (ON) Foundation Hip Review
Hernigou, J., VERDONK, P. C. M., Homma, Y., Verdonk, R. E., Goodman, S. B., & Hernigou, P. (2021) · Arthroscopy The Journal of Arthroscopic and Related Surgery · doi:10.1016/j.arthro.2021.08.032 · Cited by 15 · see citing works
Effects of a protective agent on freeze-dried platelet-rich plasma
Shi, L., Li, R., Shuzhen, W., Zhou, M., Li, L., Lin, F., Li, Y., Guo, Z., Zhang, W., Chen, M., et al. (2019) · Blood Coagulation & Fibrinolysis · doi:10.1097/mbc.0000000000000796 · Cited by 10 · see citing works
Die Entgrenzung der Politik. Internationale Beziehungen und Friedensforschung.
Albert, M., Schoch, B., & Moltmann, B. (2004) · PUB – Publications at Bielefeld University (Bielefeld University) · Cited by 9 · see citing works
Development and assessment of biologically compatible anterior cruciate ligament using braided ultra‐high molecular weight polyethylene
Balasubramaniyan, K., Bhoobalan, K., Jayaraman, D., Sounderraj, S., Muthuukumar, K. R., & Santhini, E. (2021) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.35001 · Cited by 7 · see citing works
Nanosurgical and Bioengineering Treatment of Human Anterior Cruciate Ligament Tears with Ultrasound-Guided Injection of Modified Platelet-Rich Plasma Using Human Cell Memory Based on Clinical, Ultrasound, MRI, and Nanoscope Analyses: A Double-Blind Randomized Trial
Wasilczyk, C. (2024) · Journal of Clinical Medicine · doi:10.3390/jcm13092475 · Cited by 4 · see citing works
Biofunctionalization of UHMWPE Polymer and Its Application in Anterior Cruciate Ligament ReconstructionPreprint
Wahed, S. B., Dunstan, C. R., Boughton, P. C., & Ruys, A. J. (2020) · Preprints.org · doi:10.20944/preprints202008.0071.v1 · Cited by 4 · see citing works
Cell Death in Acute Organ Injury and Fibrosis
Yasuma, T., & Gabazza, E. C. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25073930 · Cited by 3 · see citing works
The Role of Platelet Rich Plasma Injections in Cases of Stress IncontinencePreprint
Tahoon, A. S., Salem, H. E.-D. H., & Mousa, A. A. A. (2022) · doi:10.32388/kg77zq.2 · Cited by 3 · see citing works
The Role of Platelet Rich Plasma Injections in Cases of Stress IncontinencePreprint
Tahoon, A. S., Salem, H. E.-D. H., & Mousa, A. A. A. (2022) · Qeios · doi:10.32388/kg77zq.4 · Cited by 3 · see citing works
Comparison of the effectiveness of subacromial administration of platelet-rich plasma and hyaluronic acid in chronic shoulder pain caused by damage to the tendons of the rotator cuff muscles according to a 6-month open clinical study
Nesterenko, V. А., Karateev, A. E., Макаров, М. А., Byalik, E. I., Makarov, S. A., & Lila, A. M. (2020) · Rheumatology Science and Practice · doi:10.47360/1995-4484-2020-570-576 · Cited by 2 · see citing works
The Role of Platelet Rich Plasma Injections in Cases of Stress IncontinencePreprint
Tahoon, A. S., Salem, H. E.-D. H., & Mousa, A. A. A. (2022) · doi:10.32388/kg77zq.3 · Cited by 2 · see citing works
Biologic Treatment of Ligament Injuries by the Sports Physician
Pogorzelski, J., Kennedy, M. I., & LaPrade, R. F. (2019) · Springer eBooks · doi:10.1007/978-3-030-10433-7_43 · Cited by 1 · see citing works
The Role of Platelet Rich Plasma Injections in Cases of Stress IncontinencePreprint
Tahoon, A. S., Salem, H. E.-D. H., & Mousa, A. A. A. (2022) · doi:10.32388/kg77zq · Cited by 1 · see citing works
The Effects of Age and Skeletal Maturity on Healing of the Anterior Cruciate Ligament
Chao, L. H., & Murray, M. M. (2013) · Springer eBooks · doi:10.1007/978-1-4614-0760-7_22
Self-citation
The Role of Orthobiologics for the Management of Ligament and Muscle Injuries in Sports
Laver, L., Samitier, G., Gilat, R., García-Balletbó, M., López-Vidriero, E., Cole, B. J., & Cugat, R. (2020) · Springer eBooks · doi:10.1007/978-3-662-61070-1_46
Treatment of Soft Tissue Injury in Athletes by Biological Method
Vardzukashvili, M., Vardzukashvili, I., Egnatieva, I., & Saganelidze, K. (2024) · doi:10.52340/spectri.2023.08.02.11
Embryology and Histology of the Anterior Cruciate Ligament of the Knee in Relation to the Transition of Structure and Function Through Ages
Rosenberg, N., & Gritsyuk, A. A. (2025) · Springer eBooks · doi:10.1007/978-3-031-84501-7_1

Platelets and plasma proteins are both required to stimulate collagen gene expression by anterior cruciate ligament cells in three-dimensional culture
Cheng, M., Wang, H., Yoshida, R., & Murray, M. M. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0199 · Cited by 62 · see citing works
Self-citation
Human anterior cruciate ligament fibroblasts from immature patients have a stronger in vitro response to platelet concentrates than those from mature individuals
Magarian, E. M., Vavken, P., & Murray, M. M. (2010) · The Knee · doi:10.1016/j.knee.2010.05.015 · Cited by 35 · see citing works
Self-citation
Age dependence of expression of growth factor receptors in porcine ACL fibroblasts
Vavken, P., Saad, F. A., & Murray, M. M. (2010) · Journal of Orthopaedic Research® · doi:10.1002/jor.21111 · Cited by 55 · see citing works
Self-citation
Can Platelet rich plasma stimulate human ACL growth in culture? A preliminary experience
Aspenberg, M. S., Karna, S., Dhatt, S. S., Behera, P., & Bhatia, A. (2019) · Muscles Ligaments and Tendons Journal · doi:10.32098/mltj.03.2015.03
Increasing platelet concentration in platelet‐rich plasma inhibits anterior cruciate ligament cell function in three‐dimensional culture
Yoshida, R., Cheng, M., & Murray, M. M. (2014) · Journal of Orthopaedic Research · doi:10.1002/jor.22493 · Cited by 67 · see citing works
Self-citation

Authors: Cheng, M., Johnson, V. M., & Murray, M. M.
Affiliations: Boston Children's Hospital (US)
Corresponding author: Murray, Martha M.
ORCID: Cheng, Mingyu
Murray, Martha M.
Venue: Journal of Orthopaedic Research
Publisher: Wiley
Published: 2011-07-11
Volume: 30
Issue: 1
Pages: 79-85
ISSN: 0736-0266 (ISSN-L)
1554-527X
DOI: 10.1002/jor.21496
PMC: PMC3202019
OpenAlex: W2010895172
Cited by: 40
References: 38
Citations by year: 2025: 1 · 2024: 3 · 2023: 2 · 2022: 7 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 3 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 1
FWCI: 2.31
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Journal of Orthopaedic Research® (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health
National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR054099)
Topics: Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
Health Sciences › Medicine › Orthopedics and Sports Medicine › Tendon Structure and Treatment
Health Sciences › Medicine › Surgery › Knee injuries and reconstruction techniques
Keywords: platelet-rich plasma, anterior cruciate ligament, age-dependent effects, type I collagen, collagen gene expression, cell viability, apoptosis, cellular metabolic activity, growth factor responsiveness, collagen gel, ligament healing, skeletal maturity, porcine model
MeSH terms: Age Factors, Aging, Animals, Cell Division, Cell Survival, Cells, Cultured, Energy Metabolism, Fibroblasts, Swine, Swine, Miniature, Gene Expression, Anterior Cruciate Ligament, Apoptosis, Collagen Type I, Collagen Type III, Platelet-Rich Plasma, Tissue Scaffolds

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Effects of age and platelet-rich plasma on ACL cell viability and collagen gene expression

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks