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An improved enzymic method for the determination of native and modified starch
Abstract
An improved method for the determination of starch by sequential hydrolysis with thermostable bacterial α‐amylase and fungal amyloglucosidase is described. Glucose was determined colorimetrically by a glucose oxidase‐peroxidase‐chromogen system at pH 7. Native normal and waxy starches, and distarch phosphate, gave quantitative yields of glucose with a high degree of precision (coefficient of variation less than 1%). Acetylated distarch phosphate, high‐amylose starch and retrograded amylose were initially treated with 1M NaOH for 30 min, then neutralised and analysed successfully as normal starch. Oxidised starch did not give a quantitative yield of glucose because of the presence of dicarboxylic groups in the polymer. For samples containing normal and waxy starch the analysis was carried out in about 4 h. The method was applied to a range of starch‐containing foodstuffs and the results are reported.
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Citations
Cited by
Morphology and functional properties of corn, potato and tapioca starches
MISHRA, S., & RAI, T. (2006) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2005.01.001 · Cited by 332
Oba, M., & Allen, M. S. (2000) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(00)75000-4 · Cited by 234
Tester, R. F., Debon, S. J. J., & Karkalas, J. (1998) · Journal of Cereal Science · doi:10.1016/s0733-5210(98)90006-6 · Cited by 118
Self-citationWeiss, W. P., & Wyatt, D. J. (2000) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(00)74886-7 · Cited by 109
Laarman, A. H., Ruiz-Sanchez, A. L., Sugino, T., Guan, L. L., & Oba, M. (2012) · Journal of Dairy Science · doi:10.3168/jds.2011-4788 · Cited by 101
Metadata
- Authors
- Karkalas, J.
- Venue
- Journal of the Science of Food and Agriculture
- Publisher
- Wiley
- Published
- 1985-10
- Volume
- 36
- Issue
- 10
- Pages
- 1019-1027
- ISSN
- 0022-5142 (ISSN-L)
- 1097-0010
- OpenAlex
- W2079817409
- Cited by
- 355
- References
- 0
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
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- Karkalas, J. (1985). An improved enzymic method for the determination of native and modified starch. Journal of the Science of Food and Agriculture. https://doi.org/10.1002/jsfa.2740361018
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