CoChat
Sign Up
Cited by 58

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

OncomiR miR-96 and miR-182 promote cell proliferation and invasion through targeting ephrinA5 in hepatocellular carcinoma

Wang, Tong-Hong, Yeh, Chau-Ting, Ho, Jar-Yi, Ng, Kwai-Fong, Chen, Tse-Ching·2016·Molecular Carcinogenesis
DOI 10.1002/mc.22286
Open this paper in CoChat — free account

Abstract

EphrinA5, a member of the ephrinA subclass, is downregulated in hepatocellular carcinoma (HCC) and acts as a tumor suppressor. However, the upstream regulation mechanism of ephrinA5 remains unclear. In this study, we tried to identify and characterize the roles of miR-96 and miR-182 in the regulation of ephrinA5 expression in HCC. The expression levels of miR-96 and miR-182 were examined in 47 paired HCC and para-tumoral liver tissues using quantitative real-time RT-PCR. The luciferase reporter assay and western blotting were employed to dissect the association between miR-96/182 and ephrinA5 expression. Moreover, cells were treated with synthetic miR-96/182 precursors and inhibitors to assess their effects on HCC cell growth and migration. It was found that both miR-96 and miR-182 were upregulated in HCC compared to para-tumoral normal tissues. The expression of miR-96 and miR-182 was inversely associated with ephrinA5 protein levels. Furthermore, both miR-96 and miR-182 directly targeted the 3'UTR of the ephrinA5 mRNA and suppressed protein translation. The suppression of miR-96 and miR-182 led to reduced HCC cell proliferation and migration by negatively regulating ephrinA5 expression. In conclusion, miR-96 and miR-182 may act as oncomiRs in HCC by suppressing the expression of ephrinA5 and may play important roles in hepatocarcinogenesis. © 2015 Wiley Periodicals, Inc.

Overview

Integrity signals

Registry

Crossref · 10.1002/mc.22286

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 40

  1. Keng, 2013, Sex bias occurrence of hepatocellular carcinoma in Poly7 molecular subclass is associated with EGFR, Hepatology, 57, 120, 10.1002/hep.26004 · DOI
  2. Tai, 2009, Long-term outcome of hepatitis B e antigen-negative hepatitis B surface antigen carriers in relation to changes of alanine aminotransferase levels over time, Hepatology, 49, 1859, 10.1002/hep.22878 · DOI
  3. Yeh, 2010, Hepatitis B virus-DNA level and basal core promoter A1762T/G1764A mutation in liver tissue independently predict postoperative survival in hepatocellular carcinoma, Hepatology, 52, 1922, 10.1002/hep.23898 · DOI
  4. Mathurin, 1998, Review article: Overview of medical treatments in unresectable hepatocellular carcinoma-an impossible meta-analysis, Aliment Pharmacol Ther, 12, 111, 10.1046/j.1365-2036.1998.00286.x · DOI
  5. Kawano, 2009, Prognosis of patients with intrahepatic recurrence after hepatic resection for hepatocellular carcinoma: A retrospective study, Eur J Surg Oncol, 35, 174, 10.1016/j.ejso.2008.01.027 · DOI
  6. Raza, 2014, Hepatocellular carcinoma review: Current treatment, and evidence-based medicine, World J Gastroenterol, 20, 4115, 10.3748/wjg.v20.i15.4115 · DOI
  7. Thomas, 2009, Molecular targeted therapy for hepatocellular carcinoma, J Gastroenterol, 19, 136, 10.1007/s00535-008-2252-z · DOI
  8. Croce, 2005, MiRNAs, cancer, and stem cell division, Cell, 122, 6, 10.1016/j.cell.2005.06.036 · DOI
  9. Dykxhoorn, 2010, MicroRNAs and metastasis: Little RNAs go a long way, Cancer Res, 70, 6401, 10.1158/0008-5472.CAN-10-1346 · DOI
  10. Esteller, 2011, Non-coding RNAs in human disease, Nature Rev Genet, 12, 861, 10.1038/nrg3074 · DOI · Paper page
  11. Mateescu, 2011, MiR-141 and miR-200a act on ovarian tumorigenesis by controlling oxidative stress response, Nat Med, 17, 1627, 10.1038/nm.2512 · DOI · Paper page
  12. Dagan, 2012, MiR-155 regulates HGAL expression and increases lymphoma cell motility, Blood, 119, 13, 10.1182/blood-2011-08-370536 · DOI
  13. Fang, 2012, MicroRNA-7 inhibits tumor growth and metastasis by targeting the phosphoinositide 3-kinase/Akt pathway in hepatocellular carcinoma, Hepatology, 55, 1852, 10.1002/hep.25576 · DOI
  14. Wang, 2009, MicroRNA and cancer-focus on apoptosis, J Cell Mol Med, 13, 12, 10.1111/j.1582-4934.2008.00510.x · DOI
  15. Zhang, 2013, Meta-analysis of microRNA-183 family expression in human cancer studies comparing cancer tissues with noncancerous tissues, Gene, 527, 26, 10.1016/j.gene.2013.06.006 · DOI
  16. Nie, 2012, MicroRNA-365, down-regulated in colon cancer, inhibits cell cycle progression and promotes apoptosis of colon cancer cells by probably targeting Cyclin D1 and Bcl-2, Carcinogenesis, 33, 220, 10.1093/carcin/bgr245 · DOI
  17. Navon, 2009, Novel rank-based statistical methods reveal microRNAs with differential expression in multiple cancer types, PLoS One, 4, e8003, 10.1371/journal.pone.0008003 · DOI
  18. Lin, 2010, Unregulated miR-96 induces cell proliferation in human breast cancer by downregulating transcriptional factor FOXO3a, PLoS One, 5, e15797, 10.1371/journal.pone.0015797 · DOI
  19. Guttilla, 2009, Coordinate regulation of FOXO1 by miR-27a, miR-96, and miR-182 in breast cancer cells, J Biol Chem, 284, 23204, 10.1074/jbc.M109.031427 · DOI
  20. Li, 2009, EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor, Oncogene, 28, 1759, 10.1038/onc.2009.15 · DOI
  21. Wang, 2012, Peritumoral small ephrinA5 isoform level predicts the postoperative survival in hepatocellular carcinoma, PLoS One, 7, e41749, 10.1371/journal.pone.0041749 · DOI
  22. Wang, 2012, EphrinA5 suppresses colon cancer development by negatively regulating epidermal growth factor receptor stability, FEBS J, 279, 251, 10.1111/j.1742-4658.2011.08419.x · DOI
  23. Ali, 2012, MicroRNA profiling of diagnostic needle aspirates from patients with pancreatic cancer, Br J Cancer, 107, 1354, 10.1038/bjc.2012.383 · DOI
  24. Aydogdu, 2012, MicroRNA-regulated gene networks during mammary cell differentiation are associated with breast cancer, Carcinogenesis, 33, 1502, 10.1093/carcin/bgs161 · DOI
  25. Xue, 2013, MicroRNAs as diagnostic markers for pancreatic ductal adenocarcinoma and its precursor, pancreatic intraepithelial neoplasm, Cancer Genet, 206, 217, 10.1016/j.cancergen.2013.05.020 · DOI
  26. Weeraratne, 2012, Pleiotropic effects of miR-183∼96∼182 converge to regulate cell survival, proliferation and migration in medulloblastoma, Acta neuropathol, 123, 539, 10.1007/s00401-012-0969-5 · DOI
  27. Liu, 2012, MiR-182 overexpression in tumourigenesis of high-grade serous ovarian carcinoma, J Pathol, 228, 204, 10.1002/path.4000 · DOI
  28. Moskwa, 2011, MiR-182-mediated downregulation of BRCA1 impacts DNA repair and sensitivity to PARP inhibitors, Mol Cell, 41, 210, 10.1016/j.molcel.2010.12.005 · DOI
  29. Yan, 2012, Role of microRNA-182 in posterior uveal melanoma: regulation of tumor development through MITF, BCL2 and cyclin D2. PloS one, 7, e40967, 10.1371/journal.pone.0040967 · DOI
  30. Kunz, 2013, MicroRNAs in melanoma biology, Adv Exp Med Biol, 774, 103, 10.1007/978-94-007-5590-1_6 · DOI
  31. Wang, 2012, MicroRNA-182 downregulates metastasis suppressor 1 and contributes to metastasis of hepatocellular carcinoma, BMC Cancer, 12, 227, 10.1186/1471-2407-12-227 · DOI
  32. Xu, 2013, Inhibition of miR-96 expression reduces cell proliferation and clonogenicity of HepG2 hepatoma cells, Oncol Rep, 29, 653, 10.3892/or.2012.2138 · DOI
  33. Chen, 2012, Suppression of microRNA-96 expression inhibits the invasion of hepatocellular carcinoma cells, Mol Med Rep, 5, 800
  34. Suzuki, 2013, Epigenetic alteration and microRNA dysregulation in cancer, Front Genet, 4, 258, 10.3389/fgene.2013.00258 · DOI
  35. Saito, 2006, Specific activation of microRNA-127 with downregulation of the proto-oncogene BCL6 by chromatin-modifying drugs in human cancer cells, Cancer Cell, 9, 435, 10.1016/j.ccr.2006.04.020 · DOI
  36. Suzuki, 2011, Genome-wide profiling of chromatin signatures reveals epigenetic regulation of MicroRNA genes in colorectal cancer, Cancer Res, 71, 5646, 10.1158/0008-5472.CAN-11-1076 · DOI
  37. Baer, 2012, Extensive promoter DNA hypermethylation and hypomethylation is associated with aberrant microRNA expression in chronic lymphocytic leukemia, Cancer Res, 72, 3775, 10.1158/0008-5472.CAN-12-0803 · DOI
  38. Fornari, 2012, In hepatocellular carcinoma miR-519d is up-regulated by p53 and DNA hypomethylation and targets CDKN1A/p21, PTEN, AKT3 and TIMP2, J Pathol, 227, 275, 10.1002/path.3995 · DOI
  39. Marson, 2008, Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells, Cell, 134, 521, 10.1016/j.cell.2008.07.020 · DOI
  40. Wang, 2012, MicroRNA-224 is up-regulated in hepatocellular carcinoma through epigenetic mechanisms, FASEB J, 26, 3032, 10.1096/fj.11-201855 · DOI

Cited by

Sorted by citations

See all 58

Metadata

Authors
Wang, T.-H., Yeh, C.-T., Ho, J.-Y., Ng, K.-F., & Chen, T.-C.
Affiliations
  • Tissue Bank; Chang Gung Memorial Hospital; Tao-Yuan Taiwan
  • Liver Research Center; Department of Hepato-Gastroenterology; Chang Gung Memorial Hospital; Tao-Yuan Taiwan
  • Department of Pathology and Graduate Institute of Pathology and Parasitology; Tri-Service General Hospital; National Defense Medical Center; Taipei Taiwan
  • Department of Anatomic Pathology; Chang Gung Memorial Hospital; Chang Gung University School of Medicine; Tao-Yuan Taiwan
Venue
Molecular Carcinogenesis
Publisher
Wiley
Published
2015-02-08
Volume
55
Issue
4
Pages
366-375
ISSN
0899-1987
DOI
10.1002/mc.22286
Cited by
58
References
2
Language
en
Licence
Funders
  • National Science Council, Taiwan (103-2320-B-182A-003)
  • Chang Gung Medical Research Program, Taiwan (CMRPG3D1321, CMRPG3A1183, CMRPG391494)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review