GAP‐43 mRNA in growth cones is associated with HuD and ribosomes
Abstract
The neuron-specific ELAV/Hu family member, HuD, interacts with and stabilizes GAP-43 mRNA in developing neurons, and leads to increased levels of GAP-43 protein. As GAP-43 protein is enriched in growth cones, it is of interest to determine if HuD and GAP-43 mRNA are associated in developing growth cones. HuD granules in growth cones are found in the central domain that is rich in microtubules and ribosomes, in the peripheral domain with its actin network, and in filopodia. This distribution of HuD granules in growth cones is dependent on actin filaments but not on microtubules. GAP-43 mRNA is localized in granules found in both the central and peripheral domains, but not in filopodia. Ribosomes were extensively colocalized with HuD and GAP-43 mRNA granules in the central domain, consistent with a role in the control of GAP-43 mRNA stability in the growth cone. Together, these results demonstrate that many of the components necessary for GAP-43 mRNA translation/stabilization are present within growth cones.
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HuD is localized to granules, along with other proteins such as KIF3A and SMN, within the cytoplasm and neurites.
“Closer inspection of intraneuronal compartments revealed that HuD is localized to granules, along with other proteins, such as kinesin-like protein (KIF3A) and survival of motoneuron (SMN), within the cytoplasm and neurites (Aronov et al. 2002; Smith et al. 2004; Tiruchinapalli et al. 2008a,b; Hubers et al. 2010; Akten et al. 2011; Fallini et al. 2012b).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Smith et al. 2004
GAP-43 and Tau colocalize with HuD protein and polysomes in growth cones during neuronal differentiation.
“For example, two target mRNAs of HuD involved in axonal outgrowth, namely GAP-43 and Tau, were found to colocalize with HuD protein and polysomes in growth cones during neuronal differentiation (Smith et al. 2004; Atlas et al. 2007).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Smith et al. 2004
Cites
Tsai, K.-C., Cansino, V. V., Kohn, D. T., Neve, R. L., & Perrone-Bizzozero, N. I. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-06-01950.1997 · Cited by 66 · see citing works
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Defining a neuron: neuronal ELAV proteins
Pascale, A., Amadio, M., & Quattrone, A. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7017-y · Cited by 158 · see citing works
De Rubeis, S., & Bagni, C. (2010) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2009.09.013 · Cited by 126 · see citing works
Neuronal ELAV proteins enhance mRNA stability by a PKCα-dependent pathway
Pascale, A., Amadio, M., Scapagnini, G., Lanni, C., Racchi, M., Provenzani, A., Govoni, S., Alkon, D. L., & Quattrone, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504702102 · Cited by 129 · see citing works
Bronicki, L. M., & Jasmin, B. J. (2013) · RNA · doi:10.1261/rna.039164.113 · Cited by 126 · see citing works
DeBoer, E. M., Azevedo, R., Vega, T. A., Brodkin, J., Akamatsu, W., Okano, H., Wagner, G. C., & Rasin, M.-R. (2014) · The Journal of Neuroscience · doi:10.1523/jneurosci.3703-13.2014 · Cited by 72 · see citing works
RNA–Binding Protein HuD as a Versatile Factor in Neuronal and Non–Neuronal Systems
Jung, M., & Lee, E. K. (2021) · Biology · doi:10.3390/biology10050361 · Cited by 39 · see citing works
- Authors
- Smith, C. L., Afroz, R., Bassell, G. J., Furneaux, H. M., Perrone‐Bizzozero, N. I., & Burry, R. W.
- Affiliations
- The Ohio State University (US)
- Albert Einstein College of Medicine (US)
- University of Connecticut (US)
- University of New Mexico (US)
- Corresponding author
- Burry, Richard W.
- Venue
- Journal of Neurobiology
- Publisher
- Wiley
- Published
- 2004-07-15
- Volume
- 61
- Issue
- 2
- Pages
- 222-235
- ISSN
- 0022-3034 (ISSN-L)
- 1097-4695
- OpenAlex
- W2052496682
- Cited by
- 81
- References
- 1
- Citations by year
- 2026: 2 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 5 · 2012: 9
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurobiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01gm055599-04)
- National Institute of General Medical Sciences (R01 GM55599)
- National Institute of Neurological Disorders and Stroke (R01NS030255, R01 NS30255)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- GAP-43, HUD, mRNA stability, growth cones, ribosome, mRNA localization, F-actin, microtubules, filopodia, neuronal development, mRNA translation
- MeSH terms
- Animals, Nerve Tissue Proteins, Ribosomes, RNA, Messenger, RNA-Binding Proteins, PC12 Cells, GAP-43 Protein, Growth Cones, Nerve Growth Factor, Rats, ELAV Proteins
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