Mechanisms of Artemisia scoparia ’s Anti‐Inflammatory Activity in Cultured Adipocytes, Macrophages, and Pancreatic β‐Cells
Open accessAbstract
Objective: An ethanolic extract of Artemisia scoparia (SCO) improves adipose tissue function and reduces negative metabolic consequences of high‐fat feeding. A. scoparia has a long history of medicinal use across Asia and has anti‐inflammatory effects in various cell types and disease models. The objective of the current study was to investigate SCO’s effects on inflammation in cells relevant to metabolic health. Methods: Inflammatory responses were assayed in cultured adipocytes, macrophages, and insulinoma cells by quantitative polymerase chain reaction, immunoblotting, and NF‐κB reporter assays. Results: In tumor necrosis factor α–treated adipocytes, SCO mitigated ERK and NF‐κB signaling as well as transcriptional responses but had no effect on fatty acid–binding protein 4 secretion. SCO also reduced levels of deleted in breast cancer 1 protein in adipocytes and inhibited inflammatory gene expression in stimulated macrophages. Finally, in pancreatic β‐cells, SCO decreased NF‐κB–responsive promoter activity induced by IL‐1β treatment. Conclusions: SCO’s ability to promote adipocyte development and function is thought to mediate its insulin‐sensitizing actions in vivo . Our findings that SCO inhibits inflammatory responses through at least two distinct signaling pathways (ERK and NF‐κB) in three cell types known to contribute to metabolic disease reveal that SCO may act more broadly than previously thought to improve metabolic health.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
SCO seems to interfere with the inflammatory pathway, reducing metabolic dysfunction, and has a favorable effect on pancreatic β-cells.
“Furthermore, SCO seems to interfere with the inflammatory pathway, thus reducing metabolic dysfunction, and has a favorable effect on pancreatic β-cells [31].”
From The Role of Resveratrol Administration in Human Obesity (2021) · cites this paper as [31]
Impaired adipocyte differentiation is associated with inflammatory processes in adipose tissue, which are well-known pathogenetic aspects of obesity.
“Impaired adipocyte differentiation, moreover, is also associated with inflammatory processes in the adipose tissue that are well-known pathogenetic aspects of obesity [31].”
From The Role of Resveratrol Administration in Human Obesity (2021) · cites this paper as [31]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Obesity is associated with macrophage accumulation in adipose tissue
Weisberg, S. P., McCann, D., Desai, M., Rosenbaum, M., Leibel, R. L., & Ferrante, A. W. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319246 · Cited by 10,041 · see citing works
NF-κB, Inflammation, and Metabolic Disease
Baker, R. G., Hayden, M. S., & Ghosh, S. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2010.12.008 · Cited by 1,952 · see citing works
Obesity-associated improvements in metabolic profile through expansion of adipose tissue
Kim, J.-Y., van de Wall, E., Laplante, M., Azzara, A., Trujillo, M. E., Hofmann, S. M., Schraw, T., Durand, J. L., Li, H., Li, G., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci31021 · Cited by 1,310 · see citing works
The Definition and Prevalence of Obesity and Metabolic Syndrome.
Engin, A. (2017) · Advances in Experimental Medicine and Biology · doi:10.1007/978-3-319-48382-5_1 · Cited by 978 · see citing works
Hohmeier, H. E., Mulder, H., Chen, G., Henkel-Rieger, R., Prentki, M., & Newgard, C. B. (2000) · Diabetes · doi:10.2337/diabetes.49.3.424 · Cited by 922 · see citing works
Vaure, C., & Liu, Y. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00316 · Cited by 842 · see citing works
Cawthorn, W. P., & Sethi, J. K. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.11.051 · Cited by 792 · see citing works
Targeting adipose tissue in the treatment of obesity-associated diabetes
Kusminski, C. M., Bickel, P. E., & Scherer, P. E. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.75 · Cited by 678 · see citing works
Achieving Stability of Lipopolysaccharide-Induced NF-κB Activation
Covert, M. W., Leung, T., Gaston, J. E., & Baltimore, D. (2005) · Science · doi:10.1126/science.1112304 · Cited by 603 · see citing works
Xu, A., Wang, Y., Xu, J. Y., Stejskal, D., Tam, S., Zhang, J., Wat, N. M. S., Wong, W. K., & Lam, K. S. (2006) · Clinical Chemistry · doi:10.1373/clinchem.2005.062463 · Cited by 597 · see citing works
Doyle, S., & O'Neill, L. A. J. (2006) · Biochemical Pharmacology · doi:10.1016/j.bcp.2006.07.010 · Cited by 594 · see citing works
Failure of adipocyte differentiation causes type II diabetes mellitus?
Danforth, E. (2000) · Nature Genetics · doi:10.1038/79111 · Cited by 487 · see citing works
Adipocyte lipolysis and insulin resistance
Morigny, P., Houssier, M., Mouisel, É., & Langin, D. (2015) · Biochimie · doi:10.1016/j.biochi.2015.10.024 · Cited by 471 · see citing works
The Pathogenesis of Obesity-Associated Adipose Tissue Inflammation
Engin, A. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-48382-5_9 · Cited by 291 · see citing works
Transcriptional Control of the TNF Gene
Falvo, J. V., Tsytsykova, A. V., & Goldfeld, A. E. (2010) · Current directions in autoimmunity · doi:10.1159/000289196 · Cited by 284 · see citing works
Adipocyte Lipid Chaperone aP2 Is a Secreted Adipokine Regulating Hepatic Glucose Production
Cao, H., Sekiya, M., Ertunc, M. E., Burak, M. F., Mayers, J. R., White, A., Inouye, K., Rickey, L., Erçal, B., Furuhashi, M., et al. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.04.012 · Cited by 276 · see citing works
NF-κB and STAT1 control CXCL1 and CXCL2 gene transcription
Burke, S. J., Lu, D., Sparer, T. E., Masi, T. J., Goff, M. R., Karlstad, M. D., & Collier, J. J. (2013) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00347.2013 · Cited by 174 · see citing works
Self-citationErtunc, M. E., Sikkeland, J., Fenaroli, F., Griffiths, G., Daniels, M. P., Cao, H., Saatcioglu, F., & Hotamışlıgil, G. S. (2014) · Journal of Lipid Research · doi:10.1194/jlr.m055798 · Cited by 155 · see citing works
Metformin; a review of its history and future: from lilac to longevity
Thomas, I. H., & Gregg, B. E. (2017) · Pediatric Diabetes · doi:10.1111/pedi.12473 · Cited by 140 · see citing works
The Induction of Lipocalin-2 Protein Expression in Vivo and in Vitro
Zhao, P., Elks, C. M., & Stephens, J. M. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.532234 · Cited by 98 · see citing works
Self-citationMita, T., Furuhashi, M., Hiramitsu, S., Ishii, J., Hoshina, K., Ishimura, S., Fuseya, T., Watanabe, Y. S., Tanaka, M., Ohno, K., et al. (2014) · Obesity · doi:10.1002/oby.20954 · Cited by 96 · see citing works
Hussain, W., Badshah, L., Ullah, M., et al. (2018) · Journal of Ethnobiology and Ethnomedicine · doi:10.1186/s13002-018-0229-4 · Cited by 89 · see citing works
IL-1β reciprocally regulates chemokine and insulin secretion in pancreatic β-cells via NF-κB
Burke, S. J., Stadler, K., Lu, D., Gleason, E. L., Han, A., Donohoe, D. R., Rogers, R. C., Hermann, G. E., Karlstad, M. D., & Collier, J. J. (2015) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00153.2015 · Cited by 82 · see citing works
Self-citationSTAT1, NF-κB and ERKs play a role in the induction of lipocalin-2 expression in adipocytes
Zhao, P., & Stephens, J. M. (2013) · Molecular Metabolism · doi:10.1016/j.molmet.2013.04.003 · Cited by 73 · see citing works
Self-citationHabib, M., & Waheed, I. (2012) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2012.10.022 · Cited by 72 · see citing works
The Ubiquitin Ligase Siah2 Regulates PPARγ Activity in Adipocytes
Kilroy, G. E., Kirk-Ballard, H., Carter, L. E., & Floyd, Z. E. (2012) · Endocrinology · doi:10.1210/en.2011-1725 · Cited by 68 · see citing works
Transcriptional Activation of Inflammatory Genes: Mechanistic Insight into Selectivity and Diversity
Ahmed, A. U., Williams, B. R. G., & Hannigan, G. E. (2015) · Biomolecules · doi:10.3390/biom5043087 · Cited by 61 · see citing works
Regulation of iNOS Gene Transcription by IL-1β and IFN-γ Requires a Coactivator Exchange Mechanism
Burke, S. J., Updegraff, B. L., Bellich, R. M., Goff, M. R., Lu, D., Minkin, S. C., Karlstad, M. D., & Collier, J. J. (2013) · Molecular Endocrinology · doi:10.1210/me.2013-1159 · Cited by 59 · see citing works
Self-citationWang, Z. Q., Zhang, X. H., Yu, Y., Tipton, R. C., Raskin, I., Ribnicky, D., Johnson, W., & Cefalu, W. T. (2013) · Metabolism · doi:10.1016/j.metabol.2013.03.004 · Cited by 58 · see citing works
Self-citationHussain, W., Ullah, M., Dastagir, G., et al. (2018) · PubMed · doi:10.22038/ajp.2018.20910.1792 · Cited by 58 · see citing works
Lipocalin‐2—The myth of its expression and function
Li, D., Sun, W. Y., Fu, B., Xu, A., & Wang, Y. (2019) · Basic & Clinical Pharmacology & Toxicology · doi:10.1111/bcpt.13332 · Cited by 54 · see citing works
Deleted in breast cancer-1 (DBC-1) in the interface between metabolism, aging and cancer
Chini, E. N., Chini, C. C. S., Nin, V., & Escande, C. (2013) · Bioscience Reports · doi:10.1042/bsr20130062 · Cited by 45 · see citing works
Inducible nitric oxide synthase modulates lipolysis in adipocytes
Penfornis, P., & Marette, A. (2004) · Journal of Lipid Research · doi:10.1194/jlr.m400344-jlr200 · Cited by 40 · see citing works
Pancreatic β‐Cell production of CXCR3 ligands precedes diabetes onset
Burke, S. J., Karlstad, M. D., Eder, A. E., Regal, K. M., Lu, D., Burk, D. H., & Collier, J. J. (2016) · BioFactors · doi:10.1002/biof.1304 · Cited by 40 · see citing works
Self-citationCho, J., Park, K., Hwang, D., Chanmuang, S., Jaiswal, L., Park, Y.-K., Park, S., Kim, S.-Y., Kim, H.-R., Moon, J., et al. (2015) · Molecules · doi:10.3390/molecules201119657 · Cited by 35 · see citing works
Richard, A. J., Burris, T. P., Sánchez‐Infantes, D., Wang, Y., Ribnicky, D., & Stephens, J. M. (2014) · Nutrition · doi:10.1016/j.nut.2014.02.013 · Cited by 34 · see citing works
Self-citationTranscriptional Regulation of Chemokine Genes: A Link to Pancreatic Islet Inflammation?
Burke, S. J., & Collier, J. J. (2015) · Biomolecules · doi:10.3390/biom5021020 · Cited by 33 · see citing works
Self-citationRichard, A. J., Fuller, S., Fedorcenco, V., Beyl, R., Burris, T. P., Mynatt, R., Ribnicky, D. M., & Stephens, J. M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0098897 · Cited by 30 · see citing works
Self-citationWang, X., Huang, H., Ma, X., et al. (2018) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2018.05.054 · Cited by 29 · see citing works
Boudreau, A., Poulev, A., Ribnicky, D., Raskin, I., Thirumurugan, R., Richard, A. J., & Stephens, J. M. (2019) · Frontiers in Nutrition · doi:10.3389/fnut.2019.00018 · Cited by 25 · see citing works
Self-citationMoreno‐Navarrete, J. M., Moreno, M., Vidal, M., Ortega, F. J., Ricart, W., & Fernández‐Real, J. M. (2015) · Obesity · doi:10.1002/oby.20999 · Cited by 24 · see citing works
Escande, C., Nin, V., Pirtskhalava, T., Chini, C. C. S., Tchkonia, T., Kirkland, J. L., & Chini, E. N. (2014) · Diabetes · doi:10.2337/db14-0192 · Cited by 24 · see citing works
Nam, S., Han, N., Rah, S., et al. (2017) · Immunopharmacology and Immunotoxicology · doi:10.1080/08923973.2017.1405438 · Cited by 23 · see citing works
Kong, S., Dong, H., Song, J. J., Thiruppathi, M., Prabhakar, B. S., Qiu, Q., Lin, Z., Chini, E. N., Zhang, B., & Fang, D. (2015) · The Journal of Immunology · doi:10.4049/jimmunol.1500713 · Cited by 19 · see citing works
Gilani, A. H., & Janbaz, K. H. (1994) · PubMed · Cited by 18 · see citing works
Boudreau, A., Richard, A. J., Burrell, J. A., King, W. T., Dunn, R., Schwarz, J., Ribnicky, D., Rood, J. C., Salbaum, J. M., & Stephens, J. M. (2018) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00177.2018 · Cited by 17 · see citing works
Self-citationPromyo, K., Cho, J., Park, K., et al. (2017) · Food Science and Biotechnology · doi:10.1007/s10068-017-0077-3 · Cited by 12 · see citing works
Loss of DBC1 (CCAR2) affects TNFα-induced lipolysis and Glut4 gene expression in murine adipocytes
Able, A., Richard, A. J., & Stephens, J. M. (2018) · Journal of Molecular Endocrinology · doi:10.1530/jme-18-0154 · Cited by 8 · see citing works
Self-citationDeleted in breast cancer 1 plays a functional role in adipocyte differentiation
Moreno‐Navarrete, J. M., Moreno, M., Vidal, M., Ortega, F. J., Serrano, M., Xifra, G., Ricart, W., & Fernández‐Real, J. M. (2015) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00286.2014 · Cited by 6 · see citing works
Evaluation of Artemisia scoparia for hemostasis promotion activity
Sajid, M., Khan, M. R. R., Shah, N. A., et al. (2017) · Pakistan Journal of Pharmaceutical Sciences · doi:10.36721/pjps/30/5/05.09.2017/4141/1709-1713 · Cited by 4 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Role of Resveratrol Administration in Human Obesity
Mongioì, L. M., La Vignera, S., Cannarella, R., Cimino, L., Compagnone, M., Condorelli, R. A., & Calogero, A. E. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22094362 · Cited by 71 · see citing works
- Authors
- Boudreau, A., Burke, S. J., Collier, J. J., Richard, A. J., Ribnicky, D. M., & Stephens, J. M.
- Affiliations
- Pennington Biomedical Research Center (US)
- Rutgers, The State University of New Jersey (US)
- Corresponding author
- Stephens, Jacqueline M.
- ORCID
- Venue
- Obesity
- Publisher
- Wiley
- Published
- 2020-08-02
- Volume
- 28
- Issue
- 9
- Pages
- 1726-1735
- ISSN
- 1930-7381 (ISSN-L)
- 1930-739X
- PMC
- PMC7483878
- OpenAlex
- W3046515102
- Cited by
- 14
- References
- 50
- Citations by year
- 2026: 3 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 3
- FWCI
- 1.43
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Obesity (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Civil War Book Review (submittedVersion)
- Funders
- Office of Dietary Supplements (P50 AT002776)
- National Institutes of Health (NIH 2P30DK072476)
- Directorate for Biological Sciences
- Nutrition Obesity Research Center, University of North Carolina (NIH 2P30‐DK072476)
- National Institute of General Medical Sciences (P20GM103528, U54GM104940)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK072476)
- National Center for Complementary and Integrative Health (P50AT002776)
- Topics
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Natural Antidiabetic Agents Studies
- Keywords
- Artemisia scoparia, anti-inflammatory activity, adipocytes, macrophages, pancreatic beta cells, NF-κB signaling, ERK signaling, TNF-α, IL-1β, DBC1, FABP4, insulin sensitivity, metabolic disorders, high-fat feeding, ethanolic plant extract
- MeSH terms
- Animals, Anti-Inflammatory Agents, Disease Models, Animal, Humans, Inflammation, Macrophages, Transfection, Adipocytes, Artemisia, Scoparia, Insulin-Secreting Cells, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
