New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

Mohammadi, Mohammad; Mashayekh, Tooba; Rashidi‐Monfared, Sajad; Ebrahimi, Amin; Abedini, Davar

doi:10.1002/pca.2887

Cited by 46Plant biochemistry and biosynthesisPhytochemical Studies and BioactivitiesPlant Gene Expression Analysis

New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

Mohammadi, Mohammad, Mashayekh, Tooba, Rashidi‐Monfared, Sajad, Ebrahimi, Amin, Abedini, Davar·2020·Phytochemical Analysis

DOI 10.1002/pca.2887

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Abstract

INTRODUCTION: Throughout history, thousands of medicinal and aromatic plants have been widely utilised by people worldwide. Owing to them possessing of valuable compounds with little side effects in comparison with chemical drugs, herbs have been of interest to humans for a number of purposes. Diosgenin, driven from fenugreek, Trigonella foenum-graecum L., has extensively drawn scientist's attention owing to having curable properties and being a precursor of steroid hormones synthesis. Nonetheless, complete knowledge about the biosynthesis pathway of this metabolite is still elusive. OBJECTIVE: In the present research, we isolated the full-length CDS of 14 genes involving in diosgenin formation and measured their expression rate in various genotypes, which had illustrated different amount of diosgenin. METHODOLOGY: The genes were successfully isolated, and functional motifs were also assessed using in silico approaches. RESULTS: -reductase, which converts cycloartenol to cycloartanol, is the first-committed and rate-limiting enzyme in this pathway. Additionally, no transcripts indicating to the presence or expression of lanosterol synthase were detected, contradicting the previous hypothesis about the biosynthetic pathway of diosgenin in fenugreek. CONCLUSION: Considering all these, therefore, we propose the most possible pathway of diosgenin. This knowledge will then pave the way toward cloning the genes as well as engineering the diosgenin biosynthesis pathway.

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Trigonelline is an important chemotaxonomic marker of Trigonella species.
“Trigonelline is an important chemotaxonomic marker of Trigonella species (Mohammadi et al., 2020;Shailajan et al., 2011;Zameer et al., 2018) and was found to be the major compound in the sample analysed herein.”
From Multi-target neuroprotective effects of herbal medicines for Alzheimer's disease (2022) · cites this paper as Mohammadi et al., 2020

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Authors: Mohammadi, M., Mashayekh, T., Rashidi‐Monfared, S., Ebrahimi, A., & Abedini, D.
Affiliations: Tarbiat Modares University (IR)
Agricultural Biotechnology Department, Faculty of Agriculture Tarbiat Modares University Tehran Iran
University of Shahrood (IR)
ORCID: Rashidi‐Monfared, Sajad
Ebrahimi, Amin
Venue: Phytochemical Analysis
Publisher: Wiley
Published: 2019-08-30
Volume: 31
Issue: 2
Pages: 229-241
ISSN: 0958-0344 (ISSN-L)
1099-1565
DOI: 10.1002/pca.2887
OpenAlex: W2970374077
Cited by: 46
References: 60
Citations by year: 2026: 3 · 2025: 11 · 2024: 5 · 2023: 11 · 2022: 6 · 2021: 3 · 2020: 7
FWCI: 1.32
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Phytochemical Analysis (publishedVersion)
PubMed (publishedVersion)
Funders: Tarbiat Modares University
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
Keywords: diosgenin, fenugreek, lanosterol synthase, gene expression, full-length cDNA, metabolic engineering
MeSH terms: Diosgenin, Humans, Plant Extracts, Trigonella, Biosynthetic Pathways

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New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

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