Antiviral antibody profiling by high‐density protein arrays
Open accessAbstract
Viral infections elicit antiviral antibodies and have been associated with various chronic diseases. Detection of these antibodies can facilitate diagnosis, treatment of infection, and understanding of the mechanisms of virus‐associated diseases. In this work, we assayed antiviral antibodies using a novel high‐density nucleic acid programmable protein array (HD‐NAPPA) platform. Individual viral proteins were expressed in situ directly from plasmids encoding proteins in an array of microscopic reaction chambers. Quality of protein display and serum response was assured by comparing intra‐ and inter‐array correlation within or between printing batches with average correlation coefficients of 0.91 and 0.96, respectively. HD‐NAPPA showed higher signal‐to‐background ratio compared with standard NAPPA on planar glass slides and ELISA. Antibody responses to 761 antigens from 25 different viruses were profiled among patients with juvenile idiopathic arthritis and type 1 diabetes. Common and unique antibody reactivity patterns were detected between patients and healthy controls. We believe HD‐viral‐NAPPA will enable the study of host–pathogen interactions at unprecedented dimensions and elucidate the role of pathogen infections in disease development.
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The LaBaer lab has since released a panviral proteome set of 2035 open reading frame clones generated from 830 viral genes from both DNA and RNA viruses.
“Available as a cloning resource, the LaBaer lab has since released a panviral proteome set of 2035 open reading frame clones generated from 830 viral genes from both DNA and RNA viruses, including HCMV, HSV-1, KSHV, vaccinia virus, SINV, chikungunya virus, and yellow fever virus [104,111].”
From Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection (2016) · cites this paper as [111]
The technology has been advantageously employed to profile antiviral antibodies produced by infected cells on a high-density NAPPA array of viral antigens.
“Additionally, the technology has been advantageously employed to profile antiviral antibodies produced by infected cells on a high-density NAPPA array of viral antigens [111].”
From Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection (2016) · cites this paper as [111]
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- Authors
- Bian, X., Wiktor, P., Kahn, P., Brunner, A., Khela, A., Karthikeyan, K., Barker, K., Yu, X., Magee, M., Wasserfall, C. H., Gibson, D., Rooney, M. E., Qiu, J., & LaBaer, J.
- Affiliations
- Arizona State University (US)
- Engineering Arts (United States) (US)
- University of Florida (US)
- University of Ulster (GB)
- Queen's University Belfast (GB)
- Corresponding author
- Qiu, Ji
- LaBaer, Joshua
- ORCID
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2015-05-15
- Volume
- 15
- Issue
- 12
- Pages
- 2136-2145
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- PMC
- PMC4545592
- OpenAlex
- W2105857986
- Cited by
- 31
- References
- 45
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 7 · 2016: 5 · 2015: 2
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- PROTEOMICS (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Research Portal (Queen's University Belfast) (submittedVersion)
- Funders
- Juvenile Diabetes Research Foundation (5-2012-537)
- National Institute of Health (R42GM106704)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- antiviral antibodies, antibody profiling, juvenile idiopathic arthritis, type 1 diabetes, host-pathogen interaction, viral infection, autoimmunity
- MeSH terms
- Antibodies, Viral, Arthritis, Juvenile, Autoantibodies, Child, Preschool, Diabetes Mellitus, Type 1, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Nucleic Acids, Viral Proteins, Biomarkers, Case-Control Studies, Protein Array Analysis, Proteomics, Immunoprecipitation, Host-Pathogen Interactions
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