Next-generation high-density self-assembling functional protein arrays
Open accessAbstract
We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
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The LaBaer lab has developed protein microarrays containing printed complementary DNA expression vectors, the proteins of which are expressed de novo on the chip.
“To circumvent this challenge, the LaBaer lab has developed protein microarrays containing printed complementary DNA expression vectors, the proteins of which are expressed de novo on the chip [109,110].”
From Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection (2016) · cites this paper as [110]
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Self-Assembling Protein Microarrays
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- Authors
- Ramachandran, N., Raphael, J. V., Hainsworth, E., Demirkan, G., Fuentes, M. G., Rolfs, A., Hu, Y., & LaBaer, J.
- Affiliations
- Harvard University (US)
- Corresponding author
- LaBaer, Joshua
- Venue
- Nature Methods
- Published
- 2008-05-11
- Volume
- 5
- Issue
- 6
- Pages
- 535-538
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC3070491
- OpenAlex
- W2013787405
- Cited by
- 303
- References
- 15
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 8 · 2023: 4 · 2022: 8 · 2021: 11 · 2020: 13 · 2019: 10 · 2018: 18 · 2017: 17 · 2016: 20 · 2015: 24 · 2014: 25 · 2013: 24 · 2012: 26
- FWCI
- 12.49
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Public Health Service (HHSN2332200400053C)
- National Cancer Institute (U01CA117374)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Keywords
- nucleic acid programmable protein arrays, protein immobilization
- MeSH terms
- Albumins, Animals, Cattle, Cell-Free System, Cloning, Molecular, Fluorescent Dyes, Glutathione Transferase, Humans, DNA, Complementary, Peptide Library, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Protein Array Analysis, Proteomics
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