A comparison of the immunogenicity of a pair of enantiomeric proteins
Abstract
The immunogenicity of a folded, all D‐amino acid protein‐ rubredoxin, has been compared with that for the corresponding L‐protein enantiomer. Following multiple administrations with alum adjuvant, the L‐protein induced a strong, specific lgG antibody response, whereas the D‐protein did not. This relative lack of responsiveness to the D‐protein cannot be attributed to rapid excretion, since it is retained at least 4 times longer than the natural L‐protein. These observations provide the first direct evidence that a folded D‐amino acid protein has low immunogenicity and is long lived in vivo. Proteins with such properties may be useful as molecular platforms in a variety of chemical and pharmaco‐logical applications. © 1993 Wiley‐Liss, Inc.
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- Authors
- Dintzis, H. M., Symer, D. E., Dintzis, R. Z., Zawadzke, L. E., & Berg, J. M.
- Affiliations
- Johns Hopkins University (US)
- Corresponding author
- Dintzis, Howard M.
- Venue
- Proteins: Structure, Function, and Bioinformatics
- Publisher
- Wiley
- Published
- 1993-07
- Volume
- 16
- Issue
- 3
- Pages
- 306-308
- ISSN
- 0887-3585 (ISSN-L)
- 1097-0134
- OpenAlex
- W2167125107
- Cited by
- 104
- References
- 13
- Citations by year
- 2026: 8 · 2025: 5 · 2024: 9 · 2023: 4 · 2022: 4 · 2021: 6 · 2020: 4 · 2019: 6 · 2018: 5 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 4 · 2012: 3
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Proteins Structure Function and Bioinformatics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Keywords
- rubredoxin, immunogenicity, IgG antibody response, alum adjuvant, protein excretion
- MeSH terms
- Adjuvants, Immunologic, Alum Compounds, Animals, Antibody Formation, Female, Mice, Inbred Strains, Rubredoxins, Stereoisomerism, Structure-Activity Relationship, Mice
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