The activity against leukocyte eicosanoid generation of essential oil and polar fractions of Adesmia boronioides Hook.f.
Abstract
The polar fractions (methanol and aqueous extract) and essential oil of Adesmia boronioides aerial parts were tested in vitro at concentrations of 15 and 50 microg/mL, for their effects on the COX and 5-LOX pathways of eicosanoid generation (TXB2, PGE2 and LTB4) in stimulated rat peritoneal leukocytes. Potent inhibition of LTB4 generation was displayed by the methanol extract and the essential oil, whereas the aqueous extract was essentially inactive. The methanol extract also caused potent inhibition of TXB2 generation but the essential oil and the aqueous extract were much less active. The effects on PGE2 production were much less striking, implying that the main effect is on thromboxane synthetase rather than on COX. Although the essential oil caused LDH release in leukocytes treated concurrently with ionophore, suggesting substantial toxicity to the cells, this extract did not affect cell viability according to the MTT test when incubated with the cells in the absence of ionophore. The two other extracts did not affect cell viability at the concentrations tested. It is concluded that Adesmia boronioides contains substance(s) that preferentially inhibit the 5-lipoxygenase activity of arachidonic acid metabolism and suggest that this may contribute to the antiinflammatory actions of extracts of this plant.
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Prostaglandin E2 amplifies pain and enhances vascular permeability, whereas leukotrienes contract vascular smooth muscle, enhance vascular permeability, and mediate proinflammatory and allergic responses.
“The prostaglandins, especially prostaglandin E2 (PGE2) amplify the pain mechanism and enhance vascular permeability whereas the leukotrienes contract the smooth muscles of blood vessels, enhance vascular permeability and mediate proinflammatory and allergic responses [97].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [97]
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Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review
Miguel, M. G. (2010) · Molecules · doi:10.3390/molecules15129252 · Cited by 933 · see citing works
“The prostaglandins, especially prostaglandin E2 (PGE2) amplify the pain mechanism and enhance vascular permeability whereas the leukotrienes contract the smooth muscles of blood vessels, enhance vascular permeability and mediate proinflammatory and allergic responses [97].”
Background
- Authors
- González, S. B., Houghton, P. J., & Hoult, J. R. S.
- Affiliations
- National University of Patagonia San Juan Bosco (AR)
- King's College London (GB)
- Corresponding author
- Houghton, P. J.
- Venue
- Phytotherapy Research
- Publisher
- Wiley
- Published
- 2003-03
- Volume
- 17
- Issue
- 3
- Pages
- 290-293
- ISSN
- 0951-418X (ISSN-L)
- 1099-1573
- OpenAlex
- W2156487821
- Cited by
- 10
- References
- 10
- Citations by year
- 2025: 1 · 2024: 1 · 2022: 1 · 2021: 1 · 2019: 2 · 2013: 1
- FWCI
- 0.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phytotherapy Research (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
- Keywords
- essential oils, 5-lipoxygenase inhibition, cyclooxygenase, thromboxane B2, prostaglandin E2, leukotriene B4, anti-inflammatory activity, peritoneal leukocytes, arachidonic acid metabolism, cytotoxicity
- MeSH terms
- Animals, Anti-Inflammatory Agents, Fabaceae, Leukocytes, Male, Phytotherapy, Plant Extracts, Plant Oils, Eicosanoids, Rats, Wistar, Rats
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