Effect of aged garlic extract against methotrexate-induced damage to the small intestine in rats
Abstract
Methotrexate (MTX) chemotherapy is often accompanied by side effects such as gastrointestinal ulceration and diarrhea. The aim of this study was to examine histologically whether an aged garlic extract (AGE) had a protective effect on the small intestine of rats with MTX‐induced damage. Forty male Wistar albino rats were randomized into experimental and control groups and divided into four groups of ten animals. To the first group, MTX was applied as a single dose (20 mg/kg) intraperitoneally. To the second group, in addition to MTX application, AGE (250 mg/kg) was administered orally every day at the same time by intragastric intubation until the rats were killed. To the third group, AGE only was given. The fourth group was the control. All animals were killed 4 days after the intraperitoneal injection of MTX for histopathologic analysis and tissue MDA levels. Before killing, intracardiac blood was obtained from each animal to perform biochemical analysis (plasma lactate level). MTX was found to lead to damage in the jejunal tissues and to increase the MDA and lactate levels in the plasma. Administration of the AGE decreased the severity of jejunal damage, but increased MDA and lactate levels caused by MTX treatment on the other hand. These results suggest that AGE may protect the small intestine of rats from MTX‐induced damage. Thus this study substantiated the thought that the protective effect of AGE is derived from the manner in which it interacts with crypt cells. Copyright © 2006 John Wiley & Sons, Ltd.
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Dehydrated raw garlic powder when administered orally resulted in acute injury to the gastric mucosa, whilst AGE protects the intestinal mucosa of experimental animals.
“Chen et al. [131] revealed that dehydrated raw garlic powder when administered orally resulted in acute injury to the gastric mucosa, whilst Yuncu et al. [132] reported that AGE, the sulfur-free compound, protects the intestinal mucosa of experimental animals.”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [132]
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- Authors
- Yüncü, M., Eralp, A., & Celõk, A.
- Affiliations
- Gaziantep University (TR)
- Corresponding author
- Yüncü, Mehmet
- Venue
- Phytotherapy Research
- Publisher
- Wiley
- Published
- 2006-04-18
- Volume
- 20
- Issue
- 6
- Pages
- 504-510
- ISSN
- 0951-418X (ISSN-L)
- 1099-1573
- OpenAlex
- W2005985203
- Cited by
- 28
- References
- 38
- Citations by year
- 2024: 2 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 1 · 2016: 1 · 2014: 2 · 2013: 5 · 2012: 1
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Phytotherapy Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Chemotherapy-induced organ toxicity mitigation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Health Sciences › Medicine › Dermatology › Chemotherapy-related skin toxicity
- Keywords
- aged garlic extract, methotrexate, small bowel injury, intestinal mucosal protection, malondialdehyde, oxidative stress, lactate, chemotherapy-induced gastrointestinal toxicity, crypt cells
- MeSH terms
- Animals, Diarrhea, Garlic, Intestine, Small, Male, Malondialdehyde, Methotrexate, Plant Extracts, Weight Loss, Rats, Wistar, Lactic Acid, Rats
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