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Peptide sequencing by matrix‐assisted laser‐desorption mass spectrometry
Abstract
A novel method of peptide sequencing by mass spectrometry is described. Metastable decay of laser‐desorbedions, taking place in the first field‐free drift region of a reflectron time‐of‐flight mass spectrometer, has been monitored to get structural information from larger peptides. Fragment ions from metastable decay are mass analysed by adjusting the potentiaals of the ion reflectron according to the kinetic energies of the ions. The features of the technique and its significance for future applications are outlined.
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Citations
Cited by
Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS
Caprioli, R. M., Farmer, T. B., & Gile, J. (1997) · Analytical Chemistry · doi:10.1021/ac970888i · Cited by 2,234
Loboda, A. V., Krutchinsky, A. N., Bromirski, M., Ens, W., & Standing, K. G. (2000) · Rapid Communications in Mass Spectrometry · doi:10.1002/1097-0231(20000630)14:12<1047::aid-rcm990>3.0.co;2-e · Cited by 305
Schnölzer, M., Jedrzejewski, P., & Lehmann, W. D. (1996) · ELECTROPHORESIS · doi:10.1002/elps.1150170517 · Cited by 295
Metadata
- Authors
- Spengler, B., Kirsch, D., Kaufmann, R., & Jaeger, E.
- Venue
- Rapid Communications in Mass Spectrometry
- Publisher
- Wiley
- Published
- 1992-02
- Volume
- 6
- Issue
- 2
- Pages
- 105-108
- ISSN
- 0951-4198 (ISSN-L)
- 1097-0231
- OpenAlex
- W1968904921
- Cited by
- 305
- References
- 0
- FWCI
- 10.03
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Peptide sequencing by matrix‐assisted laser‐desorption mass spectrometry. https://app.cochat.ai/papers/10.1002/rcm.1290060207
- The paper
- Spengler, B., Kirsch, D., Kaufmann, R., & Jaeger, E. (1992). Peptide sequencing by matrix‐assisted laser‐desorption mass spectrometry. Rapid Communications in Mass Spectrometry. https://doi.org/10.1002/rcm.1290060207
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.