The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Quantitative profiling of biomarkers related to B‐vitamin status, tryptophan metabolism and inflammation in human plasma by liquid chromatography/tandem mass spectrometry
Abstract
Vitamins B 2 and B 6 serve as cofactors in enzymatic reactions involved in tryptophan and homocysteine metabolism. Plasma concentrations of these vitamins and amino acids are related to smoking and inflammation, and correlate with other markers of immune activation. Large‐scale studies of these relations have been hampered by lack of suitable analytical methods. The assay described includes riboflavin, five vitamin B 6 forms (pyridoxal 5′‐phosphate, pyridoxal, 4‐pyridoxic acid, pyridoxine and pyridoxamine), tryptophan and six tryptophan metabolites (kynurenine, kynurenic acid, anthranilic acid, 3‐hydroxykynurenine, xanthurenic acid and 3‐hydroxyanthranilic acid), cystathionine, neopterin and cotinine. Trichloroacetic acid containing 13 isotope‐labelled internal standards was added to 60 µL of plasma, the mixture was centrifuged, and the resulting supernatant used for analysis. The analytes were separated within 5 min on a stable‐bond C8 column by a gradient‐type mobile phase containing acetonitrile, heptafluorobutyric acid and high concentration (650 mmol/L) of acetic acid, and detected using electrospray ionization tandem mass spectrometry (ESI‐MS/MS). The mobile phase ensured sufficient separation and high ionization efficiency of all analytes. Recoveries were 75–123% and within‐day and between‐day coefficients of variance (CVs) were 2.5–9.5% and 5.4–16.9%, respectively. Limits of detection ranged from 0.05 to 7 nmol/L. The method enables quantification of endogenous plasma concentrations of 16 analytes related to B‐vitamin status and inflammation, and may prove useful in large‐scale epidemiological studies. Copyright © 2009 John Wiley & Sons, Ltd.
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Cites
Matuszewski, B. K., Constanzer, M. L., & Chavez-Eng, C. M. (2003) · Analytical Chemistry · doi:10.1021/ac020361s · Cited by 5,335
Methionine metabolism in mammals
Finkelstein, J. D. (1990) · The Journal of Nutritional Biochemistry · doi:10.1016/0955-2863(90)90070-2 · Cited by 1,491
Huber, C., Batchelor, J. R., Fuchs, D., Hausen, A., Lang, A., Niederwieser, D., Reibnegger, G., Swetly, P., Troppmair, J., & Wachter, H. (1984) · The Journal of experimental medicine · doi:10.1084/jem.160.1.310 · Cited by 1,084
Pirkle, J. L. (1996) · JAMA · doi:10.1001/jama.275.16.1233 · Cited by 704
Monitoring tryptophan metabolism in chronic immune activation
Schröcksnadel, K., Wirleitner, B., Winkler, C., & Fuchs, D. (2006) · Clinica Chimica Acta · doi:10.1016/j.cca.2005.06.013 · Cited by 551
Simultaneous Measurement of Serum Tryptophan and Kynurenine by HPLC,
Widner, B., Werner, E. R., Schennach, H., Wachter, H., & Fuchs, D. (1997) · Clinical Chemistry · doi:10.1093/clinchem/43.12.2424 · Cited by 411
Bernert, J. T., Turner, W. E., Pirkle, J. L., Sosnoff, C. S., Akins, J. R., Waldrep, M. K., Ann, Q., Covey, T. R., Whitfield, W. E., Gunter, E. W., et al. (1997) · Clinical Chemistry · doi:10.1093/clinchem/43.12.2281 · Cited by 337
Elevation of serum cystathionine levels in patients with cobalamin and folate deficiency
Stabler, S., Lindenbaum, J., Savage, D., & Allen, R. (1993) · Blood · doi:10.1182/blood.v81.12.3404.3404 · Cited by 262
Melnyk, S. (1999) · The Journal of Nutritional Biochemistry · doi:10.1016/s0955-2863(99)00033-9 · Cited by 229
Tryptophan Nutrition and Metabolism: An Overview
Peters, J. C. (1991) · Advances in Experimental Medicine and Biology · doi:10.1007/978-1-4684-5952-4_32 · Cited by 211
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DNA methylation in newborns and maternal smoking in pregnancy: genome-wide consortium meta-analysis
Joubert, B. R., Felix, J. F., Yousefi, P., Bakulski, K. M., Just, A. C., Breton, C., Reese, S. E., Markunas, C. A., Richmond, R. C., Xu, C.-J., et al. (2016) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2016.02.019 · Cited by 886
Joubert, B. R., Håberg, S. E., Nilsen, R. M., Wang, X., Vollset, S. E., Murphy, S. K., Huang, Z., Hoyo, C., Midttun, Ø., Cupul-Uicab, L. A., et al. (2012) · Environmental Health Perspectives · doi:10.1289/ehp.1205412 · Cited by 716
Self-citationCancer Incidence and Mortality After Treatment With Folic Acid and Vitamin B12
Ebbing, M. (2009) · JAMA · doi:10.1001/jama.2009.1622 · Cited by 428
Inflammation, vitamin B6 and related pathways
Ueland, P. M., McCann, A., Midttun, Ø., & Ulvik, A. (2017) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2016.08.001 · Cited by 382
Self-citationTissue‐specific profiling of the Arabidopsis thaliana auxin metabolome
Novák, O., Hényková, E., Sairanen, I., Kowalczyk, M., Pospíšil, T., & Ljung, K. (2012) · The Plant Journal · doi:10.1111/j.1365-313x.2012.05085.x · Cited by 304
Metadata
- Authors
- Midttun, Ø., Hustad, S., & Ueland, P. M.
- Venue
- Rapid Communications in Mass Spectrometry
- Publisher
- Wiley
- Published
- 2009-03-31
- Volume
- 23
- Issue
- 9
- Pages
- 1371-1379
- ISSN
- 0951-4198 (ISSN-L)
- 1097-0231
- OpenAlex
- W2079670701
- Cited by
- 358
- References
- 53
- FWCI
- 8.03
- Type
- journal article
- Language
- en
- Open access
- closed
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