Fluorescent Polymer Nanoparticles for Cell Barcoding In Vitro and In Vivo
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Fluorescent polymer nanoparticles for long‐term labeling and tracking of living cells with any desired color code are developed. They are built from biodegradable poly(lactic‐co‐glycolic acid) polymer loaded with cyanine dyes (DiO, DiI, and DiD) with the help of bulky fluorinated counterions, which minimize aggregation‐caused quenching. At the single particle level, these particles are ≈20‐fold brighter than quantum dots of similar color. Due to their identical 40 nm size and surface properties, these nanoparticles are endocytosed equally well by living cells. Mixing nanoparticles of three colors in different proportions generates a homogeneous RGB (red, green, and blue) barcode in cells, which is transmitted through many cell generations. Cell barcoding is validated on 7 cell lines (HeLa, KB, embryonic kidney (293T), Chinese hamster ovary, rat basophilic leucemia, U97, and D2A1), 13 color codes, and it enables simultaneous tracking of co‐cultured barcoded cell populations for >2 weeks. It is also applied to studying competition among drug‐treated cell populations. This technology enabled six‐color imaging in vivo for (1) tracking xenografted cancer cells and (2) monitoring morphogenesis after microinjection in zebrafish embryos. In addition to a robust method of multicolor cell labeling and tracking, this work suggests that multiple functions can be co‐localized inside cells by combining structurally close nanoparticles carrying different functions.
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Using this approach, fluorescent polymer nanoparticles with varied fluorescence wavelengths have been developed.
“Using this approach, fluorescent polymer nanoparticles with varied fluorescence wavelengths have been developed (Figure 8c).155”
From The Pursuit of Shortwave Infrared-Emitting Nanoparticles with Bright Fluorescence through Molecular Design and Excited-State Engineering of Molecular Aggregates (2022) · cites this paper as [155]
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Ultrabright Fluorescent Organic Nanoparticles Based on Small‐Molecule Ionic Isolation Lattices**
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Self-citation
- Authors
- Andreiuk, B., Reisch, A., Lindecker, M., Follain, G., Peyriéras, N., Goetz, J. G., & Klymchenko, A. S.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Corresponding author
- Reisch, Andreas
- Klymchenko, Andrey S.
- ORCID
- Venue
- Small
- Publisher
- Wiley
- Published
- 2017-08-09
- Volume
- 13
- Issue
- 38
- ISSN
- 1613-6810 (ISSN-L)
- 1613-6829
- OpenAlex
- W2742904541
- Cited by
- 129
- References
- 66
- Citations by year
- 2026: 9 · 2025: 8 · 2024: 10 · 2023: 16 · 2022: 19 · 2021: 27 · 2020: 15 · 2019: 15 · 2018: 10
- FWCI
- 4.61
- Type
- journal article
- Language
- en
- Open access
- green
- zenodo.org
- Licence
- Locations
- Small (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Funders
- H2020 European Research Council (648528)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- cell barcoding, poly(lactic-co-glycolic acid), cyanine dyes, aggregation-caused quenching, cell tracking, in vivo imaging, biodegradable nanoparticles
- MeSH terms
- Animals, Carbocyanines, Cell Survival, Color, Embryo, Nonmammalian, Fluorescence, HeLa Cells, Humans, Polymers, Zebrafish, Mice, Nanoparticles, Cell Tracking
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