The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Concise Review: The Evolution of human pluripotent stem cell culture: From feeder cells to synthetic coatings
Open accessAbstract
Current practices to maintain human pluripotent stem cells (hPSCs), which include induced pluripotent stem cells and embryonic stem cells, in an undifferentiated state typically depend on the support of feeder cells such as mouse embryonic fibroblasts (MEFs) or an extracellular matrix such as Matrigel. Culture conditions that depend on these undefined support systems limit our ability to interpret mechanistic studies aimed at resolving how hPSCs interact with their extracellular environment to remain in a unique undifferentiated state and to make fate-changing lineage decisions. Likewise, the xenogeneic components of MEFs and Matrigel ultimately hinder our ability to use pluripotent stem cells to treat debilitating human diseases. Many of these obstacles have been overcome by the development of synthetic coatings and bioreactors that support hPSC expansion and self-renewal within defined culture conditions that are free from xenogeneic contamination. The establishment of defined culture conditions and synthetic matrices will facilitate studies to more precisely probe the molecular basis of pluripotent stem cell self-renewal and differentiation. When combined with three-dimensional cultures in bioreactors, these systems will also enable large-scale expansion for future clinical applications.
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Cites
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Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
Engineered materials for organoid systems
Kratochvil, M. J., Seymour, A. J., Li, T. L., Paşca, S. P., Kuo, C. J., & Heilshorn, S. C. (2019) · Nature Reviews Materials · doi:10.1038/s41578-019-0129-9 · Cited by 414
Lei, Y., & Schaffer, D. V. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1309408110 · Cited by 332
Advances and challenges in stem cell culture
McKee, C., & Chaudhry, G. R. (2017) · Colloids and Surfaces B: Biointerfaces · doi:10.1016/j.colsurfb.2017.07.051 · Cited by 323
Tissue engineering by decellularization and 3D bioprinting
Garreta, E., Oria, R., Tarantino, C., Pla-Roca, M., Prado, P., Fernández-Avilés, F., Campistol, J. M., Samitier, J., & Montserrat, N. (2017) · Materials Today · doi:10.1016/j.mattod.2016.12.005 · Cited by 263
Metadata
- Authors
- Villa-Diaz, L. G., Ross, A. M., Lahann, J., & Krebsbach, P. H.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-12-19
- Volume
- 31
- Issue
- 1
- Pages
- 1-7
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2046591218
- Cited by
- 253
- References
- 55
- FWCI
- 7.43
- Type
- journal article
- Language
- en
- Open access
- bronze
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