The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Human Induced Pluripotent Stem-Derived Retinal Pigment Epithelium (RPE) Cells Exhibit Ion Transport, Membrane Potential, Polarized Vascular Endothelial Growth Factor Secretion, and Gene Expression Pattern Similar to Native RPE
Open accessAbstract
Age-related macular degeneration (AMD) is one of the major causes of blindness in aging population that progresses with death of retinal pigment epithelium (RPE) and photoreceptor degeneration inducing impairment of central vision. Discovery of human induced pluripotent stem (hiPS) cells has opened new avenues for the treatment of degenerative diseases using patient-specific stem cells to generate tissues and cells for autologous cell-based therapy. Recently, RPE cells were generated from hiPS cells. However, there is no evidence that those hiPS-derived RPE possess specific RPE functions that fully distinguish them from other types of cells. Here, we show for the first time that RPE generated from hiPS cells under defined conditions exhibit ion transport, membrane potential, polarized vascular endothelial growth factor secretion, and gene expression profile similar to those of native RPE. The hiPS-RPE could therefore be a very good candidate for RPE replacement therapy in AMD. However, these cells show rapid telomere shortening, DNA chromosomal damage, and increased p21 expression that cause cell growth arrest. This rapid senescence might affect the survival of the transplanted cells in vivo and therefore, only the very early passages should be used for regeneration therapies. Future research needs to focus on the generation of “safe” as well as viable hiPS-derived somatic cells.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
A p53-mediated DNA damage response limits reprogramming to ensure iPS cell genomic integrity
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“Recently, the effect of viral reprogramming on induction of DNA damage and loss of DNA damage-induced G(1)/S cell cycle arrest in iPS cells has been reported [27].”
BackgroundMethodThe retinal pigment epithelium: a versatile partner in vision
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Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells
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Transcriptome analysis and molecular signature of human retinal pigment epithelium
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Stem cells in retinal regeneration: past, present and future
Ramsden, C. M., Powner, M. B., Carr, A.-J. F., Smart, M. J. K., da Cruz, L., & Coffey, P. J. (2013) · Development · doi:10.1242/dev.092270 · Cited by 253
Drusen in patient-derived hiPSC-RPE models of macular dystrophies
Galloway, C. A., Dalvi, S., Hung, S. S. C., MacDonald, L. A., Latchney, L. R., Wong, R. C. B., Guymer, R. H., Mackey, D. A., Williams, D. S., Chung, M. M., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1710430114 · Cited by 123
Plasma membrane protein polarity and trafficking in RPE cells: past, present and future
Lehmann, G. L., Benedicto, I., Philp, N. J., & Rodriguez-Boulan, E. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.04.021 · Cited by 122
Immunological aspects of RPE cell transplantation
Sugita, S., Mandai, M., Kamao, H., & Takahashi, M. (2021) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2021.100950 · Cited by 77
Murad, N., Kokkinaki, M., Gunawardena, N., Gunawan, M. S., Hathout, Y., Janczura, K. J., Theos, A. C., & Golestaneh, N. (2014) · The FEBS Journal · doi:10.1111/febs.13066 · Cited by 73
Self-citationHazim, R. A., Karumbayaram, S., Jiang, M., Dimashkie, A., Lopes, V. S., Li, D., Burgess, B. L., Vijayaraj, P., Alva-Ornelas, J. A., Zack, J. A., et al. (2017) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0652-9 · Cited by 66
Sorsby fundus dystrophy—A review of pathology and disease mechanisms
Christensen, D. R. G., Brown, F. E., Cree, A. J., Ratnayaka, J. A., & Lotery, A. J. (2017) · Experimental Eye Research · doi:10.1016/j.exer.2017.08.014 · Cited by 60
Müller, C., Charniga, C., Temple, S., & Finnemann, S. C. (2018) · Stem Cell Reports · doi:10.1016/j.stemcr.2018.01.017 · Cited by 61
Sorsby fundus dystrophy:Insights from the past and looking to the future
Anand‐Apte, B., Chao, J. R., Singh, R., & Stöhr, H. (2019) · Journal of Neuroscience Research · doi:10.1002/jnr.24317 · Cited by 45
Choi, S. W., Kim, J.-J., Seo, M.-S., Park, S.-B., Shin, T.-H., Shin, J.-H., Seo, Y., Kim, H.-S., & Kang, K.-S. (2017) · Journal of Veterinary Science · doi:10.4142/jvs.2017.18.1.59 · Cited by 21
Metadata
- Authors
- Kokkinaki, M., Sahibzada, N., & Golestaneh, N.
- Venue
- Stem Cells
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-04-19
- Volume
- 29
- Issue
- 5
- Pages
- 825-835
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W2038106981
- Cited by
- 180
- References
- 8
- FWCI
- 7.52
- Type
- journal article
- Language
- en
- Open access
- bronze
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