The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
RNA‐Seq methods for transcriptome analysis
Open accessAbstract
Deep sequencing has been revolutionizing biology and medicine in recent years, providing single base‐level precision for our understanding of nucleic acid sequences in high throughput fashion. Sequencing of RNA, or RNA‐Seq, is now a common method to analyze gene expression and to uncover novel RNA species. Aspects of RNA biogenesis and metabolism can be interrogated with specialized methods for cDNA library preparation. In this study, we review current RNA‐Seq methods for general analysis of gene expression and several specific applications, including isoform and gene fusion detection, digital gene expression profiling, targeted sequencing and single‐cell analysis. In addition, we discuss approaches to examine aspects of RNA in the cell, technical challenges of existing RNA‐Seq methods, and future directions. WIREs RNA 2017, 8:e1364. doi: 10.1002/wrna.1364 This article is categorized under: RNA Evolution and Genomics > Ribonomics RNA Methods > RNA Analyses In Vitro and In Silico
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Proteomic and interactomic insights into the molecular basis of cell functional diversity
Bludau, I., & Aebersold, R. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0231-2 · Cited by 297
Tumor microbiome links cellular programs and immunity in pancreatic cancer
Ghaddar, B., Biswas, A., Harris, C., Omary, M. B., Carpizo, D. R., Blaser, M. J., & De, S. (2022) · Cancer Cell · doi:10.1016/j.ccell.2022.09.009 · Cited by 181
Ghoreyshi, N., Heidari, R., Farhadi, A., Chamanara, M., Farahani, N., Vahidi, M., & Behroozi, J. (2025) · Discover Oncology · doi:10.1007/s12672-025-01816-9 · Cited by 91
Diagnosis of biofilm infections: current methods used, challenges and perspectives for the future
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Metadata
- Authors
- Hrdlickova, R., Toloue, M., & Tian, B.
- Venue
- WIREs RNA
- Publisher
- Wiley
- Published
- 2016-05-19
- Volume
- 8
- Issue
- 1
- ISSN
- 1757-7004 (ISSN-L)
- 1757-7012
- OpenAlex
- W2395783098
- Cited by
- 679
- References
- 25
- FWCI
- 3.29
- Type
- journal article
- Language
- en
- Open access
- green
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