Genes involved in β‐oxidation, energy metabolism and glyoxylate cycle are induced by Candida albicans during macrophage infection
Abstract
The ability of intracellular pathogens to cause infection is related to their capacity to survive and grow inside macrophages or in other cell types. Candida albicans latent virulence is likely to be related to a similar mechanism of avoiding killing by specialized cells and to the resulting ability to grow in such hostile environments. Using a differential display reverse transcription polymerase chain reaction technique, we have identified seven genes induced in C. albicans during macrophage phagocytosis. Sequence analyses and database searches revealed that these cDNAs coded for proteins homologous to yeast metabolic proteins. Interestingly, four of them are putative peroxisomal proteins, and two are involved in environmental signal sensing and transduction. Among the seven genes induced by C. albicans, six represent new information that were not described in other infection models.
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When C. albicans cells face a glucose-poor niche their metabolism changes; after phagocytosis by macrophages, repression of glycolysis occurs, and the glyoxylate cycle, gluconeogenesis, and β-oxidation are induced.
“When C. albicans cells face a glucose-poor niche their metabolism changes. For instance, after phagocytosis of C. albicans by macrophages, repression of glycolysis occurs, the glyoxylate cycle, gluconeogenesis, and β-oxidation (Fradin et al., 2003, 2005; Prigneau et al., 2003; Lorenz et al., 2004; Barelle et al., 2006; Wilson et al., 2009) are induced.”
From Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate (2015) · cites this paper as Prigneau et al., 2003
Cites
Current protocols in molecular biology
(1988) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(88)90131-4 · Cited by 4,234 · see citing works
The glyoxylate cycle is required for fungal virulence
Lorenz, M. C., & Fink, G. R. (2001) · Nature · doi:10.1038/35083594 · Cited by 745 · see citing works
Molecular enzymology of carnitine transfer and transport
Ramsay, R. R., Gandour, R. D., & van der Leij, F. R. (2001) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/s0167-4838(01)00147-9 · Cited by 387 · see citing works
Regulatory networks controlling Candida albicans morphogenesis
Brown, A. J. P., & Gow, N. A. R. (1999) · Trends in Microbiology · doi:10.1016/s0966-842x(99)01556-5 · Cited by 301 · see citing works
Transcription factors in Candida albicans – environmental control of morphogenesis
Ernst, J. F. (2000) · Microbiology · doi:10.1099/00221287-146-8-1763 · Cited by 286 · see citing works
Analysis of RNA by Northern and Slot Blot Hybridization
Brown, T. A., Mackey, K., & Du, T. (2004) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb0409s67 · Cited by 269 · see citing works
Macrophages in resistance to candidiasis
Vázquez‐Torres, A., & Balish, E. (1997) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.61.2.170-192.1997 · Cited by 233 · see citing works
Sensors of extracellular nutrients in Saccharomyces cerevisiae
Forsberg, H., & Ljungdahl, P. O. (2001) · Current Genetics · doi:10.1007/s002940100244 · Cited by 194 · see citing works
Virulence genes in the pathogenic yeastCandida albicans
Navarro-García, F., Sánchez, M., Nombela, C., & Plá, J. (2001) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2001.tb00577.x · Cited by 170 · see citing works
Transport of fatty acids and metabolites across the peroxisomal membrane
Hettema, E. H., & Tabak, H. F. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s1388-1981(00)00045-7 · Cited by 126 · see citing works
Grobler, J., Bauer, F. F., Subden, R. E., & Vuuren, H. J. J. V. (1995) · Yeast · doi:10.1002/yea.320111503 · Cited by 94 · see citing works
Swiegers, J. H., Dippenaar, N., Pretorius, I. S., & Bauer, F. F. (2001) · Yeast · doi:10.1002/yea.712 · Cited by 91 · see citing works
Sakai, Y., Murdanoto, A. P., Konishi, T., et al. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.14.4480-4485.1997 · Cited by 80 · see citing works
Transcriptional control of cell type and morphogenesis in Candida albicans
Whiteway, M. S. (2000) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(00)00144-2 · Cited by 78 · see citing works
What's New in the Mechanisms of Host Resistance to Candida Albicans Infection?
Ashman, R. B., & Papadimitriou, J. M. (1990) · Pathology - Research and Practice · doi:10.1016/s0344-0338(11)80477-2 · Cited by 32 · see citing works
Sagliocco, F. A., Guillemot, J.-C., Monribot, C., Capdevielle, J., Perrot, M., Ferrán, E. A., Ferrara, P., & Boucherie, H. (1996) · Yeast · doi:10.1002/(sici)1097-0061(199612)12:15 · Cited by 21 · see citing works
Sheridan, R., & Ratledge, C. (1996) · Microbiology · doi:10.1099/13500872-142-11-3171 · Cited by 9 · see citing works
Differential-Display Reverse Transcription-PCR (DDRT-PCR)
Colonna‐Romano, S., Leone, A., & Maresca, B. (1998) · Springer eBooks · doi:10.1007/978-3-642-80454-0 · Cited by 7 · see citing works
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Tuberculosis — metabolism and respiration in the absence of growth
Boshoff, H. I. M., & Barry, C. E. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1065 · Cited by 448 · see citing works
Niche-specific regulation of central metabolic pathways in a fungal pathogen
Barelle, C. J., Priest, C. L., MacCallum, D. M., Gow, N. A. R., Odds, F. C., & Brown, A. J. P. (2006) · Cellular Microbiology · doi:10.1111/j.1462-5822.2005.00676.x · Cited by 336 · see citing works
Ramírez, M. A., & Lorenz, M. C. (2007) · Eukaryotic Cell · doi:10.1128/ec.00372-06 · Cited by 174 · see citing works
Infection-related gene expression in Candida albicans
Brown, A. J. P., Odds, F. C., & Gow, N. A. R. (2007) · Current Opinion in Microbiology · doi:10.1016/j.mib.2007.04.001 · Cited by 148 · see citing works
Autophagy supports<i>Candida glabrata</i>survival during phagocytosis
Roetzer, A., Gratz, N., Kovarik, P., & Schüller, C. (2010) · Cellular Microbiology · doi:10.1111/j.1462-5822.2009.01391.x · Cited by 142 · see citing works
Trejo-Hernández, A., Andrade-Domínguez, A., Hernández, M., & Encarnación, S. (2014) · The ISME Journal · doi:10.1038/ismej.2014.53 · Cited by 135 · see citing works
Identifying infection-associated genes of<i>Candida albicans</i>in the postgenomic era
Wilson, D., Thewes, S., Zakikhany, K., Fradin, C., Albrecht, A., Almeida, R., Brunke, S., Grosse, K., Martin, R., Mayer, F., et al. (2009) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2009.00524.x · Cited by 115 · see citing works
Ueno, K., Matsumoto, Y., Uno, J., Sasamoto, K., Sekimizu, K., Kinjo, Y., & Chibana, H. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024759 · Cited by 109 · see citing works
A nutritive view on the host–pathogen interplay
Schaible, U. E., & Kaufmann, S. H. E. (2005) · Trends in Microbiology · doi:10.1016/j.tim.2005.06.009 · Cited by 106 · see citing works
Peroxisomal Fatty Acid β-Oxidation Is Not Essential for Virulence of <i>Candida albicans</i>
Piekarska, K., Mol, E., van den Berg, M., Hardy, G., van den Burg, J., van Roermund, C., MacCallum, D., Odds, F., & Distel, B. (2006) · Eukaryotic Cell · doi:10.1128/ec.00093-06 · Cited by 93 · see citing works
The Transcription Factor Homolog CTF1 Regulates β-Oxidation in <i>Candida albicans</i>
Ramírez, M. A., & Lorenz, M. C. (2009) · Eukaryotic Cell · doi:10.1128/ec.00206-09 · Cited by 67 · see citing works
Vieira, N., Casal, M., Johansson, B., MacCallum, D. M., Brown, A. J. P., & Paiva, S. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2009.07003.x · Cited by 63 · see citing works
MacCallum, D. M. (2009) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2009.00576.x · Cited by 56 · see citing works
Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate
Mota, S., Alves, R., Carneiro, C., Silva, S., Brown, A. J., Istel, F., Kuchler, K., Sampaio, P., Casal, M., Henriques, M., et al. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00919 · Cited by 49 · see citing works
- Authors
- Prigneau, O., Porta, A., Poudrier, J. A., Colonna‐Romano, S., Noël, T., & Maresca, B.
- Affiliations
- Institute of Genetics and Biophysics (IT)
- University of Salerno (IT)
- Université Paris Descartes (FR)
- Corresponding author
- Maresca, Bruno
- Venue
- Yeast
- Publisher
- Wiley
- Published
- 2003-05-15
- Volume
- 20
- Issue
- 8
- Pages
- 723-730
- ISSN
- 0749-503X (ISSN-L)
- 1097-0061
- OpenAlex
- W2073175848
- Cited by
- 69
- References
- 18
- Citations by year
- 2026: 2 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1 · 2016: 1 · 2015: 2 · 2014: 10 · 2012: 2
- FWCI
- 2.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Yeast (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministero dell' Università, Italy
- Ministero della Sanità-Istituto Superiore di Sanità, Progetto AIDS, Italy
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida albicans, macrophage infection, phagocytosis, gene induction, fatty acid beta-oxidation, energy metabolism, glyoxylate cycle, peroxisomal proteins, transduction, virulence, differential display
- MeSH terms
- Candida albicans, Candidiasis, Cloning, Molecular, Energy Metabolism, Genes, Fungal, Glyoxylates, Humans, Macrophages, Oxidation-Reduction, Phagocytosis, RNA, Fungal, Blotting, Northern, Gene Expression Regulation, Fungal, Polymerase Chain Reaction, Sequence Analysis, DNA, Peroxisomes, Gene Expression Profiling
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