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Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E
Open accessAbstract
A spectrophotometric method has been developed for the quantitative determination of antioxidant capacity. The assay is based on the reduction of Mo(VI) to Mo(V) by the sample analyte and the subsequent formation of a green phosphate/Mo(V) complex at acidic pH. The method has been optimized and characterized with respect to linearity interval, repetitivity and reproducibility, and molar absorption coefficients for the quantitation of several antioxidants, including vitamin E. The phosphomolybdenum method, in combination with hexane monophasic extraction, has also been adapted for the specific determination of vitamin E in seeds. The results obtained with the proposed method were validated by comparison with a standard HPLC method. The phosphomolybdenum method is routinely applied in our laboratory to evaluate the total antioxidant capacity of plant extracts and to determine vitamin E in a variety of grains and seeds, including corn and soybean.
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Cited by
Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts
Jayaprakasha, G. K., Selvi, T., & Sakariah, K. K. (2003) · Food Research International · doi:10.1016/s0963-9969(02)00116-3 · Cited by 660
Biological activities of sulfated polysaccharides from tropical seaweeds
Costa, L. S., Fidelis, G. P., Cordeiro, S. L., Oliveira, R. M., Sabry, D. A., Câmara, R. B. G., Nobre, L. T. D. B., Costa, M. S. S. P., Almeida-Lima, J., Farias, E. H. C., et al. (2010) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2009.03.005 · Cited by 659
A comparative and evolutionary approach to oxidative stress in fish: A review
Birnie‐Gauvin, K., Costantini, D., Cooke, S. J., & Willmore, W. G. (2017) · Fish and Fisheries · doi:10.1111/faf.12215 · Cited by 478
In vitro study of antioxidant activity of fruit
BANERJEE, A., DASGUPTA, N., & DE, B. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2004.04.033 · Cited by 396
Ganesan, P., Kumar, C. S., & Bhaskar, N. (2008) · Bioresource Technology · doi:10.1016/j.biortech.2007.07.005 · Cited by 393
Metadata
- Authors
- Prieto, P., Pineda, M., & Aguilar, M.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 1999-05
- Volume
- 269
- Issue
- 2
- Pages
- 337-341
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2077013120
- Cited by
- 6,221
- References
- 0
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Analytical Biochemistry. https://doi.org/10.1006/abio.1999.4019
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
