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Pulsating Fluid Flow Increases Nitric Oxide (NO) Synthesis by Osteocytes but Not Periosteal Fibroblasts - Correlation with Prostaglandin Upregulation
Abstract
Osteocytes are extremely sensitive to fluid shear stress, a phenomenon that may be related to mechanical adaptation of bone (FASEB J 9:441,1995). Here we examined the effect of pulsating fluid flow (PFF, 0.5 +/- 0.02 Pa, 5 Hz, 0.4 Pa/sec) on the release of NO, in relation with upregulation of prostaglandin E2 (PGE2). Chicken calvarial osteocytes, but not periosteal fibroblasts, as well as mouse calvarial cells responded to PFF with a rapid and transient 2 to 3-fold stimulation of NO release. The effect was maximal after 5 min and leveled off thereafter. PFF also stimulated PGE2 release. This effect was significant after 10 min and continued throughout 60 min PFF treatment. Inhibition of NO release by NG-monomethyl-L-arginine prevented the effect of PFF on NO as well as PGE2 release. These results suggest that NO is a mediator of mechanical effects in bone, leading to enhanced PGE2 release. They further strengthen the hypothesis that fluid flow through the osteocyte canalicular network provides the physical stimulus for mechanosensation in bone.
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“For example, mechanical loading stimulates osteocyte to produce factors that exert anabolic action on bone such as PGE2, prostacyclin (PGI2), NO, and IGF-1 [181–184].”
From Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells (2015) · cites this paper as [181]
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Bonewald, L. F. (2011) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.320 · Cited by 2,287
Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells
Florencio-Silva, R., Sasso, G. R. D. S., Sasso-Cerri, E., Simões, M. J., & Cerri, P. S. (2015) · BioMed Research International · doi:10.1155/2015/421746 · Cited by 2,044
The Osteocyte: An Endocrine Cell … and More
Dallas, S. L., Prideaux, M., & Bonewald, L. F. (2013) · Endocrine Reviews · doi:10.1210/er.2012-1026 · Cited by 1,042
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Burger, E. H., & Klein‐Nulend, J. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.9001.s101 · Cited by 897
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Metadata
- Authors
- Kleinnulend, J., Semeins, C. M., Ajubi, N. E., Nijweide, P. J., & Burger, E. H.
- Affiliations
- Leiden University (NL)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 217
- Issue
- 2
- Pages
- 640-648
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2043098612
- Cited by
- 425
- References
- 0
- Citations by year
- 2026: 5 · 2024: 6 · 2023: 9 · 2022: 8 · 2021: 9 · 2020: 13 · 2019: 13 · 2018: 5 · 2017: 16 · 2016: 14 · 2015: 15 · 2014: 17 · 2013: 19 · 2012: 18
- FWCI
- 2.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- VU Research Portal (publishedVersion)
- UvA-DARE (University of Amsterdam) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Keywords
- nitric oxide production, osteocytes, prostaglandin E2, fluid shear stress, mechanotransduction, calvarial cells, L-NMMA
- MeSH terms
- Animals, Cell Separation, Chick Embryo, Fibroblasts, Nitric Oxide, Osteocytes, Periodicity, Periosteum, Prostaglandins, Rheology, Skull, Stress, Mechanical, Mice
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