Characterization of Two Promoters of the Xenopus laevis elrD Gene
Abstract
The Xenopus laevis elrD gene belongs to the multigenic elav/Hu family. elrD is exclusively expressed in neural cells, where it could be involved in the posttranscriptional control of mRNAs. Here we report the isolation and characterization of the genomic elrD 5'-flanking region. We localized the transcription initiation sites and thus identified two distinct transcripts, elrD1 and elrD2 by 5' RACE PCR. The two transcripts derive from the use of alternative promoters located 915 bp apart. We show that sequences upstream of the elrD1 and elrD2 transcription units can direct expression of the reporter luciferase gene in Xenopus embryos. We also observed length variation of the ELRD first RNA recognition motif (RRM1).
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The 5′ genomic region of elrD contains two distinct promoters that drive neuron-specific expression of two E1 variants, E1 and E′1.
“In line with this possibility, the 5′ genomic region of elrD was demonstrated to contain two distinct promoters that drive neuron-specific expression of two E1 variants, E1 and E′1 (Nassar and Wegnez 2001).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Nassar and Wegnez 2001
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A conserved family of elav-like genes in vertebrates.
Good, P. J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.10.4557 · Cited by 319 · see citing works
Tsai, K.-C., Cansino, V. V., Kohn, D. T., Neve, R. L., & Perrone-Bizzozero, N. I. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-06-01950.1997 · Cited by 66 · see citing works
Samson, M.-L. (1998) · Genetics · doi:10.1093/genetics/150.2.723 · Cited by 51 · see citing works
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Bronicki, L. M., & Jasmin, B. J. (2013) · RNA · doi:10.1261/rna.039164.113 · Cited by 126 · see citing works
- Authors
- Nassar, F., & Wegnez, M.
- Affiliations
- Université Paris-Sud (FR)
- Corresponding author
- Wegnez, Maurice
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2001-05
- Volume
- 283
- Issue
- 2
- Pages
- 392-398
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2029062117
- Cited by
- 6
- References
- 3
- Citations by year
- 2013: 1 · 2012: 3
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Association pour la Recherche sur le Cancer
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- alternative promoters, 5'-flanking region, transcription start sites, RNA recognition motif, Xenopus laevis, reporter gene expression
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Luciferases, Molecular Sequence Data, Promoter Regions, Genetic, Ribonucleoproteins, Sequence Homology, Nucleic Acid, Genetic Variation, Xenopus laevis, Restriction Mapping, Alternative Splicing, Genes, Reporter, DNA Primers, Xenopus Proteins
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