Cited by 51RNA Research and SplicingDevelopmental Biology and Gene RegulationRNA and protein synthesis mechanisms

Evidence for 3′ Untranslated Region-Dependent Autoregulation of the Drosophila Gene Encoding the Neuronal Nuclear RNA-Binding Protein ELAV

Samson, Marie-Laure·1998·Genetics

Abstract

The Drosophila locus embryonic lethal abnormal visual system (elav) encodes a nuclear RNA-binding protein essential for normal neuronal differentiation and maintenance of neurons. ELAV is thought to play its role by binding to RNAs produced by other genes necessary for neuronal differentiation and consequently to affect their metabolism by an as yet unknown mechanism. ELAV structural homologues have been identified in a wide range of organisms, including humans, indicating an important conserved role for the protein. Analysis of elav germline transformants presented here shows that one copy of elav minigenes lacking a complete 3' untranslated region (3' UTR) rescues null mutations at elav, but that two copies are lethal. Additional in vivo experiments demonstrate that elav expression is regulated through the 3' UTR of the gene and indicate that this level of regulation is dependent upon ELAV itself. Because ELAV is an RNA-binding protein, the simplest model to account for these findings is that ELAV binds to the 3' UTR of its own RNA to autoregulate its expression. I discuss the implications of these results for normal elav function.

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Crossref · 10.1093/genetics/150.2.723

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Authors
Samson, M.-L.
Affiliations
University of Nebraska Medical Center (US)
Corresponding author
Samson, Marie-Laure
Venue
Genetics
Published
1998-10-01
Volume
150
Issue
2
Pages
723-733
ISSN
  • 0016-6731 (ISSN-L)
  • 1943-2631
OpenAlex
W1895804627
Cited by
51
References
54
Citations by year
2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 3 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 3
FWCI
0.70
Type
journal article
Language
en
Open access
closed
Locations
  • Genetics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
ELAV, 3' untranslated region, autoregulation, RNA-binding proteins, gene expression regulation, neuronal differentiation, Drosophila
MeSH terms
Animals, Drosophila, Female, Gene Expression Regulation, Genes, Lethal, Homeostasis, Infertility, Male, Mutation, Ribonucleoproteins, Sex Factors, Restriction Mapping, Sequence Deletion, Gene Dosage, Transgenes, 3' Untranslated Regions, Animals, Genetically Modified, ELAV Proteins

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