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The Human Papillomavirus Type 16 E5-Protein Modulates Ligand-Dependent Activation of the EGF Receptor Family in the Human Epithelial Cell Line HaCaT

Crusius, Kerstin, Auvinen, Eeva, Steuer, Barbara, Gaissert, Heike, Alonso, Angel·1998·Experimental Cell Research
DOI 10.1006/excr.1998.4024
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Abstract

The E5 open reading frame of the human papillomavirus type 16 encodes a transmembrane protein associated with the Golgi, ER, and plasma membranes. We have analyzed the effect of E5 expression on the activation of the EGF receptor family. We find that expression of the E5-protein strongly enhances EGFR activation in a ligand-dependent manner. This activation takes place immediately after addition of ligand, demonstrating that increased tyrosine phosphorylation cannot solely be due to an impaired downregulation of the receptors. Furthermore, this activation is not a result of impaired activity of EGFR-specific phosphatase through the E5-protein, as demonstrated by using inhibitors specifically blocking EGFR activation. In addition, treatment with EGF results in an enhanced activation of the ErbB2 receptor in E5-expressing cells. This superactivation must be a result of heterodimer formation between EGFR and ErbB2, since EGF is not a ligand for ErbB2. Finally, treatment of E5-expressing cells with HB-EGF shows no increased phosphorylation of the ErbB4 receptor, suggesting a specific effect of E5 on the activation of the different members of the EGFR family.

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Research works printed but not linked: 29

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Cited by

Metadata

Authors
Crusius, K., Auvinen, E., Steuer, B., Gaissert, H., & Alonso, A.
Venue
Experimental Cell Research
Publisher
Elsevier BV
Published
1998-05
Volume
241
Issue
1
Pages
76-83
ISSN
0014-4827
DOI
10.1006/excr.1998.4024
Cited by
118
References
1
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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