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The Human Papillomavirus Type 16 E5-Protein Modulates Ligand-Dependent Activation of the EGF Receptor Family in the Human Epithelial Cell Line HaCaT

Crusius, Kerstin, Auvinen, Eeva, Steuer, Barbara, Gaissert, Heike, Alonso, Angel·1998·Experimental Cell Research
DOI 10.1006/excr.1998.4024
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Abstract

The E5 open reading frame of the human papillomavirus type 16 encodes a transmembrane protein associated with the Golgi, ER, and plasma membranes. We have analyzed the effect of E5 expression on the activation of the EGF receptor family. We find that expression of the E5-protein strongly enhances EGFR activation in a ligand-dependent manner. This activation takes place immediately after addition of ligand, demonstrating that increased tyrosine phosphorylation cannot solely be due to an impaired downregulation of the receptors. Furthermore, this activation is not a result of impaired activity of EGFR-specific phosphatase through the E5-protein, as demonstrated by using inhibitors specifically blocking EGFR activation. In addition, treatment with EGF results in an enhanced activation of the ErbB2 receptor in E5-expressing cells. This superactivation must be a result of heterodimer formation between EGFR and ErbB2, since EGF is not a ligand for ErbB2. Finally, treatment of E5-expressing cells with HB-EGF shows no increased phosphorylation of the ErbB4 receptor, suggesting a specific effect of E5 on the activation of the different members of the EGFR family.

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Citations

Cites

Research works printed but not linked: 29

  1. van der Geer, 1994, Receptor protein-tyrosine kinases and their signal transduction pathways, Annu. Rev. Cell Biol., 10, 251, 10.1146/annurev.cellbio.10.1.251 · DOI
  2. Ullrich, 1990, Signal transduction by receptors with tyrosine kinase activity, Cell, 61, 203, 10.1016/0092-8674(90)90801-K · DOI · Paper page
  3. Carpenter, 1990, Epidermal growth factor, J. Biol. Chem., 265, 7709, 10.1016/S0021-9258(19)38983-5 · DOI
  4. Cano, 1995, Parallel signal processing among mammalian MAPKs, Trends Biochem. Sci., 20, 117, 10.1016/S0968-0004(00)88978-1 · DOI
  5. Feng, 1994, Phosphotyrosine phosphatses with SH2 domains: Regulators of signal transduction, Trends Gen., 10, 54, 10.1016/0168-9525(94)90149-X · DOI
  6. Wiley, 1991, The role of tyrosine kinase activity in endocytosis, compartmentation, and down-regulation of the epidermal growth factor receptor, J. Biol. Chem., 266, 11083, 10.1016/S0021-9258(18)99131-3 · DOI
  7. Baulida, 1996, All ErbB receptors other than the epidermal growth factor receptor are endocytosis impaired, J. Biol. Chem., 271, 5251, 10.1074/jbc.271.9.5251 · DOI
  8. Beerli, 1996, Epidermal growth factor-related peptides activate distinct subsets of ErbB receptors and differ in their biological activities, J. Biol. Chem., 271, 6071, 10.1074/jbc.271.11.6071 · DOI
  9. zur Hausen, 1991, Human papillomaviruses in the pathogenesis of anogenital cancer, Virology, 184, 9, 10.1016/0042-6822(91)90816-T · DOI
  10. Leechanachai, 1992, The E5 gene from the human papillomavirus type 16 is an oncogene which enhances growth factor-mediated signal transduction to the nucleus, Oncogene, 7, 19
  11. Leptak, 1991, Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16, J. Virol., 65, 7078, 10.1128/JVI.65.12.7078-7083.1991 · DOI · Paper page
  12. Bouvard, 1994, The human papillomavirus type 16 E5 gene cooperates with the E7 gene to stimulate proliferation of primary cells and increase viral gene expression, Virology, 203, 73, 10.1006/viro.1994.1456 · DOI · Paper page
  13. Conrad, 1993, The human papillomavirus type 6 and 16 E5 proteins are membrane-associated proteins which associate with the 16-kilodalton pore-forming protein, J. Virol., 67, 6170, 10.1128/JVI.67.10.6170-6178.1993 · DOI
  14. Oelze, 1995, Human papillomavirus type 16 E5 protein affects cell-cell communication in an epithelial cell line, J. Virol., 69, 4489, 10.1128/JVI.69.7.4489-4494.1995 · DOI
  15. Pim, 1992, Human papillomavirus type 16 E5 gene stimulates the transforming activity of the epidermal growth factor receptor, Oncogene, 7, 27
  16. Conrad, 1994, The E5 protein of HPV-6, but not HPV-16, associates efficiently with cellular growth factor receptors, Virology, 200, 796, 10.1006/viro.1994.1244 · DOI
  17. Hwang, 1995, The HPV16 E5 protein: Expression, detection, and stable complex formation with transmembrane proteins in COS cells, Virology, 211, 227, 10.1006/viro.1995.1395 · DOI
  18. Straight, 1995, The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes, J. Virol., 69, 3185, 10.1128/JVI.69.5.3185-3192.1995 · DOI
  19. Straight, 1993, The E5 oncoprotein of human papillomavirus type 16 transforms fibroblasts and effects the downregulation of the epidermal growth factor receptor in keratinocytes, J. Virol., 67, 4521, 10.1128/JVI.67.8.4521-4532.1993 · DOI · Paper page
  20. Böhmer, 1995, Use of selective tyrosine kinase blockers to monitor growth factor receptor dephosphorylation in intact cells, Anal. Biochem., 228, 267, 10.1006/abio.1995.1349 · DOI
  21. Stoler, 1992, Human papillomavirus type 16 and 18 gene expression in cervical neoplasias, Hum. Pathol., 23, 117, 10.1016/0046-8177(92)90232-R · DOI
  22. Bauer-Hofmann, 1996, Genomic cloning and characterization of the non-occupied allele corresponding to the integration site of human papillomavirus type 16 DNA in the cervical cancer cell line SiHa, Virology, 217, 33, 10.1006/viro.1996.0090 · DOI
  23. Kell, 1994, Detection of E5 oncoprotein in human papillomavirus type 16-positive cervical scrapes using antibodies raised to synthetic peptides, J. Gen. Virol., 75, 2451, 10.1099/0022-1317-75-9-2451 · DOI · Paper page
  24. Drummond-Barbosa, 1995, Ligand-independent activation of the platelet-derived growth factor β receptor: requirements for bovine papillomavirus E5-induced mitogenic signaling, Mol. Cell. Biol., 15, 2570, 10.1128/MCB.15.5.2570 · DOI
  25. Tomic, 1995, Association of SH2 domain protein tyrosine phosphatases with the epidermal growth factor receptor in human tumor cells: Phosphatidic acid activates receptor dephosphorylation by PTP1C, J. Biol. Chem., 270, 21277, 10.1074/jbc.270.36.21277 · DOI
  26. Knebel, 1996, Dephosphorylation of receptor tyrosine kinase as target of regulation by radiation, oxidants or alkylating agents, EMBO J., 15, 5314, 10.1002/j.1460-2075.1996.tb00916.x · DOI
  27. Elenius, 1997, Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation, EMBO J., 16, 1268, 10.1093/emboj/16.6.1268 · DOI
  28. Riese, 1996, The epidermal growth factor receptor couples transforming growth factor-α, heparin-binding epidermal growth factor-like factor, and amphiregulin to Neu, ErbB-3, and ErbB-4, J. Biol. Chem., 271, 20047, 10.1074/jbc.271.33.20047 · DOI
  29. Gamett, 1997, Secondary dimerization between members of the epidermal growth factor receptor family, J. Biol. Chem., 272, 12052, 10.1074/jbc.272.18.12052 · DOI

Cited by

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Metadata

Authors
Crusius, K., Auvinen, E., Steuer, B., Gaissert, H., & Alonso, A.
Venue
Experimental Cell Research
Publisher
Elsevier BV
Published
1998-05
Volume
241
Issue
1
Pages
76-83
ISSN
0014-4827
DOI
10.1006/excr.1998.4024
Cited by
118
References
1
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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