Exchange of retinol between IRBP and CRBP
Open accessAbstract
During a bleach, all-trans-retinol passes from the photoreceptor outer segments to the retinal pigment epithelium (RPE), where retinol is found associated with cellular retinol-binding protein (CRBP). Interphotoreceptor retinoid-binding protein (IRBP) is thought to facilitate this exchange, but the transfer of retinol between IRBP and CRBP has not been explored. In this study we used a mixture of purified IRBP and CRBP as a model system to measure the amount and rate of retinol transfer between the two proteins. When retinol is transferred from IRBP to CRBP, its absorbance maximum shifts from 330 to 350 nm. By monitoring the increase in absorbance at 350 nm after mixing CRBP with IRBP-bound retinol, we measured the amount and time course of retinol transfer from IRBP to CRBP. To complement the absorbance measurements, the IRBP and CRBP in these mixtures were subsequently separated by size-exclusion HPLC and individually analysed for retinol content by scanning the effluent with a multiple-diode-array detector. As determined by measuring the change in absorbance at 350 nm, the mean percentage of IRBP-bound retinol transferred to CRBP was 103 +/- 11% (n = 9). The mean half-time of the transfer was 4.2 +/- 1.3 sec; time to reach equilibrium was 30-60 sec. IRBP that was separated from the mixture by HPLC contained little or no retinol, while the isolated CRBP was nearly saturated with retinol. No detectable transfer from CRBP to IRBP was observed. The distribution of retinol betw…
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Soest, S. V., Westerveld, A., de Jong, P. T. V. M., Bleeker‐Wagemakers, E. M., & Bergen, A. A. B. (1999) · Survey of Ophthalmology · doi:10.1016/s0039-6257(98)00046-0 · Cited by 266 · see citing works
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- Authors
- Edwards, R. B., & Adler, A. J.
- Affiliations
- Boston University (US)
- Venue
- Experimental Eye Research
- Publisher
- Elsevier BV
- Published
- 1994-08
- Volume
- 59
- Issue
- 2
- Pages
- 161-170
- ISSN
- 0014-4835 (ISSN-L)
- OpenAlex
- W2105120922
- Cited by
- 18
- References
- 0
- Citations by year
- 2022: 2 · 2020: 2 · 2017: 1 · 2015: 1 · 2012: 1
- FWCI
- 0.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Experimental Eye Research (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ey004368-17)
- National Eye Institute (EY04368)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Keywords
- interphotoreceptor retinoid-binding protein, retinal pigment epithelium, photoreceptor outer segments, rhodopsin regeneration, retinoid-binding proteins, all-trans-retinol, photobleaching
- MeSH terms
- Retinol-Binding Proteins, Interstitial, Animals, Cattle, Chromatography, High Pressure Liquid, Eye Proteins, Kinetics, Retinol-Binding Proteins, Spectrophotometry, Vitamin A, Retinol-Binding Proteins, Cellular
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