The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Importance of Lateral and Steric Stabilization of Polyelectrolyte Gene Delivery Vectors for Extended Systemic Circulation
Open accessAbstract
Gene therapy for systemic diseases requires intravenous administration, but existing vectors are not suitable for systemic delivery, often showing rapid elimination from the bloodstream that restricts potential transfection sites to "first-pass" organs. To develop long-circulating vectors, here we have compared polyplexes containing DNA and poly-L-lysine (PLL) or polyethylenimine (PEI), surface-modified with either monovalent polyethylene glycol (PEG) or multivalent copolymers of N-(2-hydroxypropyl)methacrylamide (PHPMA), correlating their biophysical properties with their distribution following intravenous injection. A key difference between the two types of coating is the introduction of lateral stabilization by surface attachment of multivalent PHPMA, in addition to the steric stabilization provided by both types of polymers. The alpha-half-life for bloodstream clearance of polycation/DNA polyplexes (typically <5 minutes in mice) could be extended using multivalent PHPMA coating to >90 minutes. We found that the dose administered, as well as the amount and molecular weight of the coating PHPMA, had important effects on circulation properties. Multivalent PHPMA coating allows, for the first time, considerably extended circulation time using polyplex systems-a prerequisite for systemic gene delivery.
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Self-citationVector unpacking as a potential barrier for receptor-mediated polyplex gene delivery
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Cited by
Poly(ethylene glycol) in Drug Delivery: Pros and Cons as Well as Potential Alternatives
Knop, K., Hoogenboom, R., Fischer, D., & Schubert, U. S. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.200902672 · Cited by 3,533
Progress towards in Vivo Use of siRNAs
Behlke, M. A. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.01.001 · Cited by 677
Hatakeyama, H., Akita, H., Kogure, K., Oishi, M., Nagasaki, Y., Kihira, Y., Ueno, M., Kobayashi, H., Kikuchi, H., & Harashima, H. (2007) · Gene Therapy · doi:10.1038/sj.gt.3302843 · Cited by 398
Stimulus responsive nanogels for drug delivery
Zha, L., Banik, B., & Alexis, F. (2011) · Soft Matter · doi:10.1039/c0sm01307b · Cited by 391
Production and clinical development of nanoparticles for gene delivery
Chen, J., Guo, Z., Tian, H., & Chen, X. (2016) · Molecular Therapy - Methods & Clinical Development · doi:10.1038/mtm.2016.23 · Cited by 274
Metadata
- Authors
- Oupicky, D., Ogris, M., Howard, K. A., Dash, P. R., Ulbrich, K., & Seymour, L. W.
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2002-04
- Volume
- 5
- Issue
- 4
- Pages
- 463-472
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W2321451097
- Cited by
- 284
- References
- 38
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Oupicky, D., Ogris, M., Howard, K. A., Dash, P. R., Ulbrich, K., & Seymour, L. W. (2002). Importance of Lateral and Steric Stabilization of Polyelectrolyte Gene Delivery Vectors for Extended Systemic Circulation. Molecular Therapy. https://doi.org/10.1006/mthe.2002.0568
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