Five Recombinant Simian Immunodeficiency Virus Pseudotypes Lead to Exclusive Transduction of Retinal Pigmented Epithelium in Rat
Open accessAbstract
The purpose of our study was to evaluate lentiviral vector-mediated rat retinal transduction using simian immunodeficiency virus (SIV) pseudotyped with envelope proteins from vesicular stomatitis virus G glycoprotein (VSV-G), Mokola virus G protein (MK-G), amphotropic murine leukemia virus envelope (4070A-Env), influenza A virus hemagglutinin (HA), lymphocytic choriomeningitis virus G protein (LCMV-G), and RD114 retrovirus envelope (RD114-Env). The six pseudotyped lentivirus vectors carried CMV-driven green fluorescent protein (GFP) or beta-galactosidase (beta-gal) reporter genes. Intravitreal and subretinal injections of each pseudotyped recombinant SIV were performed in cohorts of Wistar rats. Our results showed that no transgene expression was detected after intravitreal injection of each pseudotyped SIV vector. Also, no transduction could be detected following subretinal injection of RD114 pseudotyped SIV vectors. However, selective transduction of retinal pigment epithelium (RPE) cells was repeatedly obtained after subretinal delivery of VSV-G, MK-G, 4070A-Env, HA, and LCMV-G pseudotyped SIV. GFP expression was maximum as soon as 4 days postadministration for VSV-G, MK-G, 4070A-Env, and HA pseudotypes, with no evidence of pseudotransduction for VSV-G. Maximum transgene expression was observed 3 weeks postinjection for LCMV-6. Importantly, HA and VSV-G pseudotyped SIV lead to such a high level of transgene expression that GFP-related toxicity occurred. Therefore, when a high level of GFP synthesis is achieved, replacement of enhanced GFP (egfp, Aequorea victoria) by a low-toxicity GFP (Renilla reniformis) cDNA is necessary to allow long-term expression.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Cites
Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-871 · Cited by 1,977 · see citing works
Gene therapy restores vision in a canine model of childhood blindness
Acland, G. M., Aguirre, G. D., Ray, J., Zhang, Q., Aleman, T. S., Cideciyan, A. V., Pearce-Kelling, S. E., Anand, V., Zeng, Y., Maguire, A. M., et al. (2001) · Nature Genetics · doi:10.1038/ng0501-92 · Cited by 1,202 · see citing works
HIV-1 genome nuclear import is mediated by a central DNA flap.
Zennou, V., Petit, C., Guétard, D., Nerhbass, U., Montagnier, L., & Charneau, P. (2000) · Cell · doi:10.1016/s0092-8674(00)80828-4 · Cited by 893 · see citing works
Cao, W., Henry, M. D., Borrow, P., Yamada, H., Elder, J. H., Ravkov, E. V., Nichol, S. T., Compans, R. W., Campbell, K. P., & Oldstone, M. B. A. (1998) · Science · doi:10.1126/science.282.5396.2079 · Cited by 692 · see citing works
Gal, A., Li, Y., Thompson, D. A., Weir, J., Orth, U., Jacobson, S. G., Apfelstedt-Sylla, E., & Vollrath, D. (2000) · Nature Genetics · doi:10.1038/81555 · Cited by 679 · see citing works
Mutations in RPE65 cause autosomal recessive childhood–onset severe retinal dystrophy
Gu, S.-M., Thompson, D. A., Srikumari, C. R. S., Lorenz, B., Finckh, U., Nicoletti, A., Murthy, K. R., Rathmann, M., Kumaramanickavel, G., Denton, M. J., et al. (1997) · Nature Genetics · doi:10.1038/ng1097-194 · Cited by 663 · see citing works
Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors
Kafri, T., Blömer, U., Peterson, D. A., Gage, F. H., & Verma, I. M. (1997) · Nature Genetics · doi:10.1038/ng1197-314 · Cited by 604 · see citing works
Kavanaugh, M. P., Miller, D. G., Zhang, W., Law, W., Kozak, S. L., Kabat, D., & Miller, A. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7071 · Cited by 585 · see citing works
Sialic Acid Species as a Determinant of the Host Range of Influenza A Viruses
Suzuki, Y., Ito, T., Suzuki, T., et al. (2000) · Journal of Virology · doi:10.1128/jvi.74.24.11825-11831.2000 · Cited by 512 · see citing works
Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector
Miyoshi, H., Takahashi, M., Gage, F. H., & Verma, I. M. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.19.10319 · Cited by 494 · see citing works
Cytokine Signals Are Sufficient for HIV-1 Infection of Resting Human T Lymphocytes
Unutmaz, D., KewalRamani, V. N., Marmon, S., & Littman, D. R. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.11.1735 · Cited by 427 · see citing works
Auricchio, A. (2001) · Human Molecular Genetics · doi:10.1093/hmg/10.26.3075 · Cited by 394 · see citing works
Kobinger, G. P., Weiner, D. J., Yu, Q., & Wilson, J. M. (2001) · Nature Biotechnology · doi:10.1038/85664 · Cited by 307 · see citing works
Ali, R. R., Sarra, G., Stephens, C., de Alwis, M., Bainbridge, J. W., Munro, P., Fauser, S., Reichel, M. B., Kinnon, C., Hunt, D. M., et al. (2000) · Nature Genetics · doi:10.1038/77068 · Cited by 302 · see citing works
Low‐affinity nerve‐growth factor receptor (P75NTR) can serve as a receptor for rabies virus
Tuffereau, C., Bénéjean, J., Blondel, D., Kieffer, B. L., & Flamand, A. (1998) · The EMBO Journal · doi:10.1093/emboj/17.24.7250 · Cited by 300 · see citing works
Schlegel, R., Tralka, T. S., Willingham, M. C., et al. (1983) · Cell · doi:10.1016/0092-8674(83)90483-x · Cited by 296 · see citing works
Correction of the retinal dystrophy phenotype of the RCS rat by viral gene transfer of <i>Mertk</i>
Vollrath, D., Feng, W., Duncan, J. L., Yasumura, D., D'Cruz, P. M., Chappelow, A., Matthes, M. T., Kay, M. A., & LaVail, M. M. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.221364198 · Cited by 290 · see citing works
Species-Specific, Postentry Barriers to Primate Immunodeficiency Virus Infection
Hofmann, W., Schubert, D., LaBonte, J. A., Munson, L., Gibson, S. V., Scammell, J. G., Ferrigno, P. K., & Sodroski, J. (1999) · Journal of Virology · doi:10.1128/jvi.73.12.10020-10028.1999 · Cited by 250 · see citing works
Follenzi, A., Sabatino, G., Lombardo, A., Boccaccio, C., & Naldini, L. (2002) · Human Gene Therapy · doi:10.1089/10430340252769770 · Cited by 245 · see citing works
Bennett, J., Maguire, A. M., Cideciyan, A. V., Schnell, M. A., Glover, E., Anand, V., Alemán, T. S., Chirmule, N. B., Gupta, A. R., Huang, Y., et al. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.17.9920 · Cited by 242 · see citing works
The RD114/simian type D retrovirus receptor is a neutral amino acid transporter
Rasko, J. E. J., Battini, J., Gottschalk, R. J., Mazo, I. A., & Miller, A. D. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.5.2129 · Cited by 220 · see citing works
Takahashi, M., Miyoshi, H., Verma, I. M., & Gage, F. H. (1999) · Journal of Virology · doi:10.1128/jvi.73.9.7812-7816.1999 · Cited by 207 · see citing works
Négre, D., Mangeot, P.-E., Duisit, G., Blanchard, S., Vidalain, P., Leissner, P., Winter, A.-J., Rabourdin‐Combe, C., Mehtali, M., Moullier, P., et al. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301292 · Cited by 206 · see citing works
Self-citationBainbridge, J., Stephens, C., Parsley, K., Demaison, C., Halfyard, A., Thrasher, A., & Ali, R. (2001) · Gene Therapy · doi:10.1038/sj.gt.3301574 · Cited by 197 · see citing works
Sanftner, L. H. M., Abel, H., Hauswirth, W. W., & Flannery, J. G. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0498 · Cited by 187 · see citing works
Liang, F.-Q., Dejneka, N. S., Cohen, D. R., Krasnoperova, N. V., Lem, J., Maguire, A. M., Dudus, L., Fisher, K. J., & Bennett, J. (2001) · Molecular Therapy · doi:10.1006/mthe.2000.0252 · Cited by 167 · see citing works
Wang, G., Slepushkin, V., Zabner, J., Keshavjee, S. H., Johnston, J., Sauter, S. L., Jolly, D. J., Dubensky, T. W., Davidson, B. L., & McCray, P. B. (1999) · Journal of Clinical Investigation · doi:10.1172/jci8390 · Cited by 157 · see citing works
Tailor, C. S., Nouri, A., Zhao, Y., Takeuchi, Y., & Kabat, D. (1999) · Journal of Virology · doi:10.1128/jvi.73.5.4470-4474.1999 · Cited by 154 · see citing works
Gasmi, M., Glynn, J. M., Jin, M., Jolly, D. J., Yee, J., & Chen, S. (1999) · Journal of Virology · doi:10.1128/jvi.73.3.1828-1834.1999 · Cited by 129 · see citing works
Mangeot, P.-E., Négre, D., Dubois, B., Winter, A. J., Leissner, P., Mehtali, M., Kaiserlian, D., Cosset, F., & Darlix, J. (2000) · Journal of Virology · doi:10.1128/jvi.74.18.8307-8315.2000 · Cited by 129 · see citing works
Self-citationLiu, M. L., Winther, B., & Kay, M. A. (1996) · Journal of Virology · doi:10.1128/jvi.70.4.2497-2502.1996 · Cited by 124 · see citing works
Production and Neurotropism of Lentivirus Vectors Pseudotyped with Lyssavirus Envelope Glycoproteins
Desmaris, N., Bosch, A., Salaün, C., Petit, C., Prévost, M., Tordo, N., Perrin, P., Schwartz, O., de ROCQUIGNY, H., & Heard, J. M. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0431 · Cited by 121 · see citing works
Pseudotyped human lentiviral vector-mediated gene transfer to airway epithelia in vivo
Johnson, L. G., Olsen, J. C., Naldini, L., & Boucher, R. C. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301138 · Cited by 104 · see citing works
Röhlich, P. (1970) · Experimental Eye Research · doi:10.1016/s0014-4835(70)80013-6 · Cited by 100 · see citing works
Acharya, S., Foletta, V. C., Lee, J. W., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.10.6945 · Cited by 93 · see citing works
Lentiviral-mediated gene transfer into human lymphocytes: role of HIV-1 accessory proteins
Chinnasamy, D., Chinnasamy, N., Enriquez, M. J., Otsu, M., Morgan, R. A., & Candotti, F. (2000) · Blood · doi:10.1182/blood.v96.4.1309 · Cited by 84 · see citing works
Marin, M., Tailor, C. S., Nouri, A., & Kabat, D. (2000) · Journal of Virology · doi:10.1128/jvi.74.17.8085-8093.2000 · Cited by 84 · see citing works
Reporter Gene Transfer Induces Apoptosis in Primary Cortical Neurons
Detrait, E., Bowers, W. J., Halterman, M. W., et al. (2002) · Molecular Therapy · doi:10.1006/mthe.2002.0609 · Cited by 69 · see citing works
Tsui, L. V., Kelly, M., Zayek, N., et al. (2002) · Nature Biotechnology · doi:10.1038/nbt0102-53 · Cited by 63 · see citing works
Wilson, C. A., Eiden, M., Anderson, W. B., et al. (1995) · Journal of Virology · doi:10.1128/jvi.69.1.534-537.1995 · Cited by 60 · see citing works
Rolling, F., Shen, W.-Y., Tabarias, H. A., Constable, I. J., Kanagasingam, Y., Barry, C. J., & Rakoczy, P. E. (1999) · Human Gene Therapy · doi:10.1089/10430349950018715 · Cited by 59 · see citing works
Self-citationDuisit, G., Salvetti, A., Moullier, P., et al. (1999) · Human Gene Therapy · doi:10.1089/10430349950018986 · Cited by 56 · see citing works
Self-citationHatziioannou, T., Valsesia‐Wittmann, S., Russell, S. J., et al. (1998) · Journal of Virology · doi:10.1128/jvi.72.6.5313-5317.1998 · Cited by 49 · see citing works
Acharya, S., Rayborn, M. E., & Hollyfield, J. G. (1998) · Glycobiology · doi:10.1093/glycob/8.10.997 · Cited by 43 · see citing works
Schnitzer, T. J., Weiss, R. A., & Závada, J. (1977) · Journal of Virology · doi:10.1128/jvi.23.3.449-454.1977 · Cited by 39 · see citing works
Lentiviral transduction of green fluorescent protein in retinal epithelium: evidence of rejection
Doi, K., Hargitai, J., Kong, J., Tsang, S. H., Wheatley, M., Chang, S., Goff, S. P., & Gouras, P. (2002) · Vision Research · doi:10.1016/s0042-6989(01)00237-1 · Cited by 34 · see citing works
Koo, H., Parthasarathi, S., Ron, Y., et al. (1994) · Virology · doi:10.1006/viro.1994.1651 · Cited by 16 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Viral vector platforms within the gene therapy landscape
Bulcha, J. T., Wang, Y., Ma, H., Tai, P. W. L., & Gao, G. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00487-6 · Cited by 1,404 · see citing works
Weber, M., Rabinowitz, J., Provost, N., Conrath, H., Folliot, S., Briot, D., Chérel, Y., Chenuaud, P., Samulski, J., Moullier, P., et al. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00098-4 · Cited by 203 · see citing works
Self-citationProvost, N., Le Meur, G., Weber, M., Mendes-Madeira, A., Podevin, G., Cherel, Y., Colle, M.-A., Deschamps, J.-Y., Moullier, P., & Rolling, F. (2005) · Molecular Therapy · doi:10.1016/j.ymthe.2004.09.022 · Cited by 87 · see citing works
Self-citationRen, D., Fisson, S., Dalkara, D., & Ail, D. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14091973 · Cited by 52 · see citing works
Ikeda, Y., Goto, Y., Yonemitsu, Y., Miyazaki, M., Sakamoto, T., Ishibashi, T., Tabata, T., Ueda, Y., Hasegawa, M., Tobimatsu, S., et al. (2003) · Gene Therapy · doi:10.1038/sj.gt.3301973 · Cited by 30 · see citing works
- Authors
- Duisit, G., Conrath, H., Saleun, S., Folliot, S., Provost, N., Cosset, F.-L., Sandrin, V., Moullier, P., & Rolling, F.
- Affiliations
- Centre Hospitalier Universitaire de Nantes (FR)
- Inserm (FR)
- Corresponding author
- Rolling, Fabienne
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2002-10
- Volume
- 6
- Issue
- 4
- Pages
- 446-454
- ISSN
- 1525-0016 (ISSN-L)
- OpenAlex
- W2316262719
- Cited by
- 79
- References
- 47
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2018: 1 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 4
- FWCI
- 4.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Therapy (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- simian immunodeficiency virus, lentiviral vectors, retinal pigment epithelium, retinal transduction, vesicular stomatitis virus glycoprotein, influenza A hemagglutinin, subretinal injection, intravitreal injection, transgene expression
- MeSH terms
- Animals, Genetic Vectors, Pigment Epithelium of Eye, Recombination, Genetic, Transduction, Genetic, Simian Immunodeficiency Virus, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
