Cited by 197Virus-based gene therapy researchRetinal Development and DisordersRNA Interference and Gene Delivery

In Vivo Gene Transfer to the Mouse Eye Using an HIV-Based Lentiviral Vector; Efficient Long-Term Transduction of Corneal Endothelium and Retinal Pigment Epithelium

Bainbridge, JWB, Stephens, C, Parsley, K, Demaison, C, Halfyard, A, Thrasher, AJ, Ali, RR·2001·Gene Therapy

Abstract

We have evaluated the transduction profiles of an HIV-based lentiviral vector delivered regionally to ocular tissues in vivo. Following subretinal injection, a green fluorescent protein (GFP) reporter gene was efficiently and stably expressed in retinal pigment epithelial (RPE) cells. Limited transduction of adjacent photoreceptors occurred in newborn mice, but was inefficient in adult animals. Injection of the vector into the anterior chamber resulted in efficient and stable transduction of corneal endothelial cells. Efficient in vivo gene transfer into cells of the corneal endothelium and retinal pigment epithelium by lentiviral vectors may therefore offer a valuable approach to the treatment of disorders of the cornea and outer retina.

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Crossref · 10.1038/sj.gt.3301574

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13 of 13 references verified0 citation errors · 0 not checkable

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  • In another study, HIV vectors carrying GFP delivered by subretinal injection showed stable expression in RPE and no inflammatory cell infiltration by immunostaining.

    “In another study, HIV vectors carrying GFP were delivered by subretinal injection-a stable expression was observed in RPE, and no inflammatory cell infiltration was observed by immunostaining [57].”

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Authors
Bainbridge, J., Stephens, C., Parsley, K., Demaison, C., Halfyard, A., Thrasher, A., & Ali, R.
Affiliations
University College London (GB)
Venue
Gene Therapy
Published
2001-11-01
Volume
8
Issue
21
Pages
1665-1668
ISSN
  • 0969-7128 (ISSN-L)
  • 1476-5462
OpenAlex
W1972583115
Cited by
197
References
13
Citations by year
2026: 4 · 2025: 2 · 2024: 6 · 2023: 2 · 2022: 9 · 2021: 6 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 8 · 2016: 3 · 2015: 10 · 2014: 6 · 2013: 14 · 2012: 7
FWCI
4.15
Type
journal article
Language
en
Open access
closed
Locations
  • Gene Therapy (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
lentiviral vectors, HIV-based vectors, subretinal injection, anterior chamber injection, retinal pigment epithelium, corneal endothelium, photoreceptors, GFP expression, corneal disease, mouse eye
MeSH terms
Animals, Animals, Newborn, Eye, Eye Diseases, Genetic Vectors, Luminescent Proteins, Mice, Inbred C57BL, Microinjections, Pigment Epithelium of Eye, Transduction, Genetic, Genetic Therapy, HIV-1, Gene Expression, Epithelium, Corneal, Green Fluorescent Proteins, Mice

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