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Cited by 224Potato Plant ResearchCarcinogens and Genotoxicity AssessmentOccupational Health and Safety in Workplaces

The DOI matches its Crossref record.

Determination of Hemoglobin Adducts in Humans Occupationally Exposed to Acrylamide

Bergmark, E., Calleman, C.J., He, F.S., Costa, L.G.·1993·Toxicology and Applied Pharmacology
DOI 10.1006/taap.1993.1085
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Abstract

Hemoglobin (Hb) adduct determinations were used to monitor occupational exposure to acrylamide (AA) and acrylonitrile (AN). Forty-one workers in a factory in the People's Republic of China who were involved in the synthesis of AA by catalytic hydration of AN and the manufacturing of polyacrylamides were studied. Ten nonexposed workers in the same city served as controls. AA and AN exposures were monitored using the modified Edman degradation procedure for the determination of their respective Hb adducts to N-terminal valine. The adduct levels in the exposed workers were 0.3-34 nmol/g Hb for AA and 0.02-66 nmol/g Hb for AN, as determined by gas chromatography-mass spectrometry (GC-MS). The formation of glycidamide (GA), the epoxide metabolite of AA, in humans was demonstrated by GC-MS analysis of its Hb adduct to N-terminal valine following acid hydrolysis, ion-exchange chromatography, and derivatization. The GA adduct was detected in samples from the exposed persons with levels of 1.6-32 nmol/g Hb. There was a linear relationship between the AA and GA adduct levels (r = 0.96) and the ratio of the in vivo doses of GA and AA was 3:10. These results suggest that AA is metabolized to GA in humans, as had previously been shown in the rat. The high AA adduct levels in the exposed workers, as compared to those expected from air concentrations, indicate that dermal exposure may contribute significantly to the total uptake of AA. The average daily in vivo doses of AA and GA in the highest exposed workers were comparable to the in vivo doses in rats injected with 3 mg/kg AA. Since a regimen of 2 mg/kg/day is known to cause a significant increase of tumors in rats, preventive measures may be necessary for humans exposed to high levels of AA in industrial settings.

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Metadata

Authors
Bergmark, E., Calleman, C. J., He, F. S., & Costa, L. G.
Affiliations
University of Washington (US)
ORCID
Costa, L.G.
Venue
Toxicology and Applied Pharmacology
Publisher
Elsevier BV
Published
1993-05
Volume
120
Issue
1
Pages
45-54
ISSN
0041-008X (ISSN-L)
DOI
10.1006/taap.1993.1085
OpenAlex
W1984526404
Cited by
221
References
0
Citations by year
2026: 5 · 2025: 2 · 2024: 3 · 2023: 6 · 2022: 5 · 2021: 3 · 2020: 7 · 2019: 7 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 5 · 2014: 9 · 2013: 4 · 2012: 10
FWCI
11.49
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Toxicology and Applied Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Occupational Health and Safety in Workplaces
Keywords
acrylamide, acrylonitrile, hemoglobin adducts, glycidamide, occupational exposure, acrylamide synthesis, dermal exposure, biomonitoring, epoxide metabolites
MeSH terms
Acrylamides, Acrylonitrile, Adult, Chromatography, Ion Exchange, Epoxy Compounds, Hemoglobins, Humans, Gas Chromatography-Mass Spectrometry, Models, Theoretical, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Structure, Occupational Exposure, Acrylamide

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