The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Simple and Rapid Preparation of Samples for PCR
Abstract
Sample preparation for PCR can be as simple and rapid as adding cells directly to the PCR. 1 Things are not quite this easy, however, for all samples and applications of PCR. In this chapter, I will discuss what determines the amount of preparation necessary and present example protocols for the rapid preparation for PCR of DNA and RNA from various sources. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Self-citationSaiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1986) · Nature · doi:10.1038/324163a0 · Cited by 1,827
DNA Amplification for Direct Detection of HIV-1 in DNA of Peripheral Blood Mononuclear Cells
Ou, C.-Y., Kwok, S., Mitchell, S. W., Mack, D. H., Sninsky, J. J., Krebs, J. W., Feorino, P., Warfield, D., & Schochetman, G. (1988) · Science · doi:10.1126/science.3336784 · Cited by 972
An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA Sequences
Kogan, S. C., Doherty, M., & Gitschier, J. (1987) · New England Journal of Medicine · doi:10.1056/nejm198710153171603 · Cited by 797
Tsukamoto, A. S., Grosschedl, R., Guzman, R. C., Parslow, T., & Varmus, H. E. (1988) · Cell · doi:10.1016/0092-8674(88)90220-6 · Cited by 747
Cited by
(1994) · PubMed · Cited by 123
Näslund, J., Fikse, W. F., Pielberg, G. R., & Lundén, A. (2008) · Journal of Dairy Science · doi:10.3168/jds.2007-0330 · Cited by 70
Mycoplasma detection by PCR analysis
Hopert, A., Uphoff, C. C., Wirth, M., Hauser, H., & Drexler, H. G. (1993) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/bf02634350 · Cited by 29
Metadata
- Authors
- Higuchi, R.
- Venue
- PCR Technology
- Publisher
- Palgrave Macmillan UK
- Published
- 1989
- Pages
- 31-38
- OpenAlex
- W2414984668
- Cited by
- 580
- References
- 5
- FWCI
- 38.92
- Type
- book chapter
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Simple and Rapid Preparation of Samples for PCR. https://app.cochat.ai/papers/10.1007/978-1-349-20235-5_4
- The paper
- Higuchi, R. (1989). Simple and Rapid Preparation of Samples for PCR. PCR Technology. https://doi.org/10.1007/978-1-349-20235-5_4
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
