In vivo and in vitro monitoring of phagosome maturation in retinal pigment epithelium cells
Abstract
The ingestion and degradation of photoreceptor disk membranes is a critical and major role for the retinal pigment epithelium (RPE). To help elucidate the cellular events involved in this role, functional in vivo and in vitro assays need to be developed further. Here we propose a method to help monitor phagosome maturation, using antibodies against different epitopes of opsin. We show that antibodies specific for the C-terminus of opsin label only immature phagosomes located in the apical region of the RPE. In contrast, antibodies recognizing the N-terminus also label more mature phagosomes, located more basally. The combined use of antibodies against different opsin epitopes thus provides a valuable tool in the study of phagosome maturation in the RPE.
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POS phagosome maturation coincides with a migration from the apical surface of the cell and then through the cell body.
“POS phagosome maturation coincides with a migration from the apical surface of the cell and then through the cell body (Herman and Steinberg, 1982; Gibbs et al., 2003; Esteve-Rudd et al., 2014; Wavre-Shapton et al., 2014).”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Esteve-Rudd et al., 2014
POS phagosomes labeled by mAb 1D4 are present in the apical RPE.
“POS phagosomes labeled by this mAb are present in the apical RPE (Esteve-Rudd et al., 2014; Wavre-Shapton et al., 2014).”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Esteve-Rudd et al., 2014
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- Authors
- Esteve-Rudd, J., Lopes, V. S., Jiang, M., & Williams, D. S.
- Affiliations
- University of California, Los Angeles (US)
- Venue
- Advances in experimental medicine and biology
- Publisher
- Springer New York
- Published
- 2014
- Volume
- 801
- Pages
- 85-90
- ISSN
- 0065-2598 (ISSN-L)
- 2214-8019
- OpenAlex
- W172778809
- Cited by
- 22
- References
- 17
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 4 · 2022: 2 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 1
- FWCI
- 11.45
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- Locations
- Advances in experimental medicine and biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Eye Institute (R01EY007042, EY07042)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- phagosome maturation, retinal pigment epithelium
- MeSH terms
- Animals, Antibody Specificity, Cells, Cultured, Phagosomes, Mice, Retinal Pigment Epithelium, Retinal Photoreceptor Cell Outer Segment, Opsins, In Vitro Techniques
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