Measurement of reactive oxygen species (ros) and mitochondrial ros in ampk knockout mice blood vessels
Open accessAbstract
Reactive oxygen species (ROS) are a group of unstable and highly reactive molecules or free radicals typically generated as by-products of cellular processes involving molecular oxygen. In vascular cells, the excessive ROS generation results in the initiation and progression of cardiovascular diseases (CVD). Therefore, a dynamic, robust, and accurate ROS detection method in the blood vessels is essential for pathophysiological research studies of the cardiovascular system.In this chapter, we describe a fluorescence dye-based detection method for assaying superoxide and mitochondrial superoxide in mouse aorta using dihydroethidium (DHE) and MitoSOX. The protocol includes preparation of frozen aortic tissue sections, monitoring DHE oxidation-derived fluorescence by fluorescence microscopy, and high-performance liquid chromatograph-based analysis of MitoSOX and its oxidation products. For studying the role of AMP-activated protein kinase (AMPK) in the redox regulation, we employed AMPKα2 knockout mice and observed increased superoxide and mitochondrial superoxide levels in the aorta of AMPK knockout mice relative to the wild-type group. This novel ROS detection method will be valuable for investigating the roles of cellular and/or mitochondrial ROS in the pathogenesis of CVDs.
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DHE reacts rather specifically with cellular O2●−, producing ethidium and 2-hydroxyethidium; it crosses membranes and can be oxidized anywhere, so only total cellular O2●− production can be detected; DNA binding can cause false localization interpretations.
“Dihydroethidium (DHE) is a red fluorescent probe that rather specifically reacts with cellular O2●−, resulting in the two fluorescent products ethidium and 2-hydroxyethidium. Only 2-hydroxyethidium is generated after reaction with O2●−, while ethidium is formed by nonspecific redox reactions. However, DHE easily crosses cellular membranes and therefore can be oxidized by O2●− anywhere in and”
From Functions of ROS in Macrophages and Antimicrobial Immunity (2021) · cites this paper as [360]
MitoSOX accumulates in the mitochondrial matrix and specifically detects O2●−, but can be oxidized by other ROS while diffusing into mitochondria; Nox2 can cause false positive signals.
“MitoSOX Red (MitoSOX) is a modified DHE analog attached to a triphenylphosphonium (TPP) group, which leads to accumulation of the probe in the mitochondrial matrix [365,366]. Like DHE itself, MitoSOX specifically detects O2●− and emits red fluorescence after the reaction (see Figure 7) [30,99,360]. In contrast to DHE, MitoSOX does so mainly in the mitochondrial matrix, though. Notably, MitoSOX can”
From Functions of ROS in Macrophages and Antimicrobial Immunity (2021) · cites this paper as [360]
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Cites
Therapeutic Targeting of Mitochondrial Superoxide in Hypertension
Dikalova, A., Bikineyeva, A. T., Budzyn, K., Nazarewicz, R. R., McCann, L. A., Lewis, W., Harrison, D. G., & Dikalov, S. (2010) · Circulation Research · doi:10.1161/circresaha.109.214601 · Cited by 737 · see citing works
Methods for Detection of Mitochondrial and Cellular Reactive Oxygen Species
Dikalov, S., & Harrison, D. G. (2012) · Antioxidants and Redox Signaling · doi:10.1089/ars.2012.4886 · Cited by 639 · see citing works
Measurement of Reactive Oxygen Species in Cardiovascular Studies
Dikalov, S., Griendling, K. K., & Harrison, D. G. (2007) · Hypertension · doi:10.1161/01.hyp.0000258594.87211.6b · Cited by 532 · see citing works
Reactive Oxygen Species: A Key Hallmark of Cardiovascular Disease
Panth, N., Paudel, K. R., & Parajuli, K. (2016) · Advances in Medicine · doi:10.1155/2016/9152732 · Cited by 405 · see citing works
Wang, Q., Zhang, M., Torres, G., Wu, S., Ouyang, C., Xie, Z., & Zou, M.-H. (2017) · Diabetes · doi:10.2337/db16-0915 · Cited by 370 · see citing works
Miller, J. D., Chu, Y., Brooks, R. M., Richenbacher, W. E., Peña-Silva, R., & Heistad, D. D. (2008) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2008.05.043 · Cited by 357 · see citing works
Wang, S., Zhang, M., Liang, B., Xu, J., Xie, Z., Liu, C., Viollet, B., Yan, D., & Zou, M.-H. (2010) · Circulation Research · doi:10.1161/circresaha.109.212530 · Cited by 296 · see citing works
Mercer, J. R., Yu, E., Figg, N. L., Cheng, K., Prime, T. A., Griffin, J. L., Masoodi, M., Vidal‐Puig, A., Murphy, M. P., & Bennett, M. R. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.11.026 · Cited by 188 · see citing works
Oak, J., & Cai, H. “. (2006) · Diabetes · doi:10.2337/db06-0288 · Cited by 157 · see citing works
Regulation of NAD(P)H oxidases by AMPK in cardiovascular systems
Song, P., & Zou, M. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.01.025 · Cited by 119 · see citing works
Self-citationQuijano, C., Castro, L., Peluffo, G., Valez, V., & Radí, R. (2007) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00761.2007 · Cited by 117 · see citing works
Zielonka, J., Srinivasan, S., Hardy, M., Ouari, O., López, M., Vasquez-Vivar, J. M., Avadhani, N. G., & Kalyanaraman, B. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.11.013 · Cited by 105 · see citing works
Cai, Y., Martens, G. A., Hinke, S. A., Heimberg, H., PIPELEERS, D. G., & de Casteele, M. V. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2006.09.018 · Cited by 99 · see citing works
Mitochondrial Cyclophilin D in Vascular Oxidative Stress and Hypertension
Itani, H. A., Dikalova, A., McMaster, W. G., Nazarewicz, R. R., Bikineyeva, A. T., Harrison, D. G., & Dikalov, S. (2016) · Hypertension · doi:10.1161/hypertensionaha.115.07085 · Cited by 86 · see citing works
Redox regulation of endothelial cell fate
Song, P., & Zou, M. (2014) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1598-z · Cited by 53 · see citing works
Self-citationExecution of Superoxide-Induced Cell Death by the Proapoptotic Bcl-2-Related Proteins Bid and Bak
Madesh, M., Zong, W.-X., Hawkins, B., Ramasamy, S., Venkatachalam, T., Mukhopadhyay, P., Doonan, P. J., Irrinki, K. M., Rajesh, M., Pacher, P., et al. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.01845-08 · Cited by 51 · see citing works
Zhu, H., Foretz, M., Xie, Z., Zhang, M., Zhu, Z., Xing, J., Leclerc, J., Gaudry, M., Viollet, B., & Zou, M. (2014) · Autophagy · doi:10.4161/auto.29197 · Cited by 38 · see citing works
Self-citationPGC-1α overexpression suppresses blood pressure elevation in DOCA-salt hypertensive mice
Zhao, Q., Zhang, J., & Wang, H. (2015) · Bioscience Reports · doi:10.1042/bsr20150076 · Cited by 17 · see citing works
Flagged first, then by citations
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Functions of ROS in Macrophages and Antimicrobial Immunity
Herb, M., & Schramm, M. (2021) · Antioxidants · doi:10.3390/antiox10020313 · Cited by 633 · see citing works
Shields, H. J., Traa, A., & Van Raamsdonk, J. M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.628157 · Cited by 478 · see citing works
Exosome-eluting stents for vascular healing after ischaemic injury
Hu, S., Li, Z., Shen, D., Zhu, D., Huang, K., Su, T., Dinh, P., Cores, J., & Cheng, K. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00705-0 · Cited by 213 · see citing works
Robertis, M. D., Sarra, A., Doria, V., Mura, F., Bordi, F., Postorino, P., & Fratantonio, D. (2020) · Biomolecules · doi:10.3390/biom10050742 · Cited by 191 · see citing works
Protective Mechanism of Humanin Against Oxidative Stress in Aging-Related Cardiovascular Diseases
Cai, H., Liu, Y., Men, H., & Zheng, Y. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.683151 · Cited by 90 · see citing works
Reactive Oxygen Species: Not Omnipresent but Important in Many Locations
Herb, M., Gluschko, A., & Schramm, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.716406 · Cited by 88 · see citing works
Marek‐Iannucci, S., Ozdemir, A. B., Moreira, D., Gomez, A. C., Lane, M. E., Porritt, R. A., Lee, Y., Shimada, K., Abe, M., Stotland, A. B., et al. (2021) · JCI Insight · doi:10.1172/jci.insight.151981 · Cited by 77 · see citing works
Alfatni, A., Riou, M., Charles, A., Meyer, A., Barnig, C., Andrès, E., Lejay, A., Talha, S., & Gény, B. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9020311 · Cited by 63 · see citing works
Zhu, T., Wang, H., Gu, H., Ju, L.-S., Wu, X., Pan, W., Zhao, M., Yang, J., & Liu, P.-M. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-01807-4 · Cited by 64 · see citing works
Hypoxia and IF1 Expression Promote ROS Decrease in Cancer Cells
Sgarbi, G., Gorini, G., Liuzzi, F., Solaini, G., & Baracca, A. (2018) · Cells · doi:10.3390/cells7070064 · Cited by 58 · see citing works
Hu, J., Luo, H., Qu, Q., Liao, X., Huang, C., Chen, J., Cai, Z., Bao, Y., Chen, G., Li, B., et al. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c11636 · Cited by 57 · see citing works
Chen, X., Mi, L., Gu, G., Gao, X., Gao, X., Shi, M., Chai, Y., Chen, F., Yang, W., & Zhang, J. (2022) · Journal of Neurotrauma · doi:10.1089/neu.2021.0347 · Cited by 57 · see citing works
In Vitro Liver Toxicity Testing of Chemicals: A Pragmatic Approach
Tabernilla, A., Rodrigues, B. D. S., Pieters, A., Caufriez, A., Leroy, K., Campenhout, R. V., Cooreman, A., Gomes, A. R., Arnesdotter, E., Gijbels, E., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22095038 · Cited by 54 · see citing works
Feng, L., Zhao, F., Liu, Q., Peng, J.-C., Duan, X., Yan, P., Wu, X., Wang, H., Deng, Y.-H., & Duan, S.-B. (2020) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2020/6123459 · Cited by 48 · see citing works
Lynch, S. E., Boyett, J. E., Smith, M. R., & Giordano‐Mooga, S. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.610516 · Cited by 44 · see citing works
Assessment of mitochondrial dysfunction and implications in cardiovascular disorders
Li, Y., Ma, Y., Dang, Q., Fan, X., Han, C., Xu, S., & Li, P. (2022) · Life Sciences · doi:10.1016/j.lfs.2022.120834 · Cited by 42 · see citing works
Shen, A. H., Borrelli, M. R., Adem, S., Deleon, N. M. D., Patel, R. A., Mascharak, S., Yen, S. J., Sun, B. Y., Taylor, W. L., Januszyk, M., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-69293-4 · Cited by 40 · see citing works
RetractedChen, H., He, X., Yu, Y., Qian, Y., Shen, J., & Zhao, S. (2020) · Talanta · doi:10.1016/j.talanta.2020.120842 · Cited by 38 · see citing works
Huang, L., Zhang, Y., Zhao, L., Chen, Q., & Li, L. (2022) · Neurocritical Care · doi:10.1007/s12028-021-01401-2 · Cited by 37 · see citing works
Wu, K., Nie, L., Nusantara, A. C., Woudstra, W., Vedelaar, T., Sigaeva, A., & Schirhagl, R. (2023) · ACS Nano · doi:10.1021/acsnano.2c08190 · Cited by 36 · see citing works
Govindappa, P. K., Talukder, M. A. H., Gurjar, A. A., Hegarty, J. P., & Elfar, J. C. (2020) · International Immunopharmacology · doi:10.1016/j.intimp.2020.106330 · Cited by 35 · see citing works
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Wang, B., Chan, Y. L., Li, G., Ho, K., Anwer, A. G., Smith, B. J., Guo, H., Jalaludin, B. B., Herbert, C. A., Thomas, P. S., et al. (2021) · Antioxidants · doi:10.3390/antiox10071029 · Cited by 36 · see citing works
Regulatory Mechanisms of Vanillic Acid in Cardiovascular Diseases: A Review
Lashgari, N., Roudsari, N. M., Momtaz, S., Abdolghaffari, A. H., Atkin, S. L., & Sahebkar, A. (2022) · Current Medicinal Chemistry · doi:10.2174/0929867329666220831152608 · Cited by 34 · see citing works
A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX
Chung, C.-Y., & Duchen, M. R. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2309-1_24 · Cited by 33 · see citing works
Lin, Y., Liu, J., Chong, S. Y., Ting, H. J., Tang, X., Yang, L., Zhang, S., Qi, X., Pei, P., Yi, Z., et al. (2024) · Small · doi:10.1002/smll.202401659 · Cited by 32 · see citing works
Cheng, S. M., Lin, T., Chang, Y.-C., Lin, I.-W., Leung, E. Y., & Cheung, C. H. A. (2021) · Pharmacological Research · doi:10.1016/j.phrs.2021.105474 · Cited by 31 · see citing works
Aklima, J., Onojima, T., Kimura, S., Umiuchi, K., Shibata, T., Kuraoka, Y., Oie, Y., Suganuma, Y., & Ohta, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.692776 · Cited by 33 · see citing works
Balta, I., Marcu, A., Linton, M., Kelly, C., Ştef, L., Peț, I., Ward, P., Pîrcălăbioru, G. G., Chifiriuc, M. C., Gundogdu, O., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-95459-9 · Cited by 33 · see citing works
Kalinovic, S., Oelze, M. W., Kröller‐Schön, S., Steven, S., Vujačić-Mirski, K., Kvandová, M., Schmal, I., Zuabi, A. A., Münzel, T. F., & Daiber, A. (2019) · Antioxidants · doi:10.3390/antiox8110514 · Cited by 31 · see citing works
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Ramos, H., Bogdanov, P. M., Huerta, J., Deàs-Just, A., Hernández, C., & Simó, R. (2022) · Antioxidants · doi:10.3390/antiox11071418 · Cited by 31 · see citing works
Christodoulou, A., Nikolaou, P., Symeonidi, L., Katogiannis, K., Pechlivani, L., Νίκου, Θ., Varela, A., Chania, C., Zerikiotis, S., Efentakis, P., et al. (2024) · Redox Biology · doi:10.1016/j.redox.2024.103311 · Cited by 27 · see citing works
Sung, J. Y., Kim, S. G., Kang, Y. J., & Choi, H. C. (2022) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2022.111708 · Cited by 28 · see citing works
Li, H., Yang, Q., Huang, Z., Liang, C., Zhang, D., Shi, H.-T., Du, J., Du, B., & Zhang, Y. (2022) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2022.09.004 · Cited by 27 · see citing works
Nagarajan, M., Maadurshni, G. B., & Manivannan, J. (2023) · Journal of Biochemical and Molecular Toxicology · doi:10.1002/jbt.23533 · Cited by 27 · see citing works
Liu, J., Chandaka, G. K., Zhang, R., & Parfenova, H. (2020) · Pharmacology Research & Perspectives · doi:10.1002/prp2.630 · Cited by 26 · see citing works
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Wang, Z., Song, Y., Jiang, J., Piao, Y., Li, L., Bai, Q., Xu, C., Liu, H., Li, L., Piao, H., et al. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.853848 · Cited by 26 · see citing works
Kashihara, T., Kawagishi, H., Nakada, T., Numaga‐Tomita, T., Kadota, S., Wolf, E. E., Du, C., Shiba, Y., Morimoto, S., & Yamada, M. (2020) · JACC Basic to Translational Science · doi:10.1016/j.jacbts.2020.08.011 · Cited by 22 · see citing works
Non-Excitatory Amino Acids, Melatonin, and Free Radicals: Examining the Role in Stroke and Aging
Carretero, V. J., Ramos, E., Segura‐Chama, P., Hernández, A., Baraibar, A. M., Álvarez‐Merz, I., Lopez-Munoz, F. J., Egea, J., Solís, J. M., Romero, A., et al. (2023) · Antioxidants · doi:10.3390/antiox12101844 · Cited by 23 · see citing works
Yakkala, P. A., Panda, S. R., Shafi, S., Naidu, V. G. M., Yar, M. S., Ubanako, P. N., Adeyemi, S. A., Kumar, P., Choonara, Y. E., Radchenko, E. V., et al. (2022) · Molecules · doi:10.3390/molecules27217642 · Cited by 22 · see citing works
Deshpande, P., Gogia, N., Chimata, A. V., & Singh, A. (2021) · BioTechniques · doi:10.2144/btn-2021-0006 · Cited by 21 · see citing works
Semyachkina-Glushkovskaya, O. V., Евгеньевич, Б. Д., Bragina, O. A., Sokolovski, S. G., Shirokov, A. A., Fedosov, I. V., Ageev, V., Андреевна, Б. И., Dubrovsky, A. I., Telnova, V., et al. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15020567 · Cited by 21 · see citing works
Ai, J., Wang, H., Chu, P., Shopit, A., Niu, M., Ahmad, N., Tesfaldet, T., Wang, F. H., Fang, J. N., Li, X., et al. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.10.003 · Cited by 21 · see citing works
Hong, L., Xue, Q., & Shao, H. J. (2021) · Journal of Inflammation Research · doi:10.2147/jir.s294809 · Cited by 21 · see citing works
Shiwakoti, S., Adhikari, D., Lee, J. P., Kang, K., Lee, I., Kim, H. J., & Oak, M. (2020) · Antioxidants · doi:10.3390/antiox9121243 · Cited by 20 · see citing works
Anti-cancer potential of (1,2-dihydronaphtho[2,1-b]furan-2-yl)methanone derivatives
Islam, K., Pal, K., Debnath, U., Basha, R. S., Khan, A. T., Jana, K., & Misra, A. K. (2020) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2020.127476 · Cited by 19 · see citing works
Shih, S., Lu, M., El‐Shazly, M., Lin, Y.-H., Chen, C., Yu, S. S. ‐., & Liu, Y. (2022) · Life · doi:10.3390/life12050687 · Cited by 19 · see citing works
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Bell, G. M., Thoma, A., Hargreaves, I. P., & Lightfoot, A. P. (2024) · Drug Safety · doi:10.1007/s40264-024-01413-9 · Cited by 21 · see citing works
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Barayan, D., Abdullahi, A., Knuth, C. M., Khalaf, F., Rehou, S., Screaton, R. A., & Jeschke, M. G. (2023) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00084.2023 · Cited by 18 · see citing works
Ibeh, S., Bakkar, N. Z., Ahmad, F. A., Nwaiwu, J. I., Barsa, C., Mekhjian, S., Reslan, M. A., Eid, A. H., Harati, H., Nabha, S. M., et al. (2022) · Life Sciences · doi:10.1016/j.lfs.2022.121316 · Cited by 15 · see citing works
Kapoor, N., Bhattacharjee, A., Chakraborty, S., & Katti, D. S. (2023) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2023.176136 · Cited by 14 · see citing works
Wang, M., Zhang, N., Li, R., Foiret, J. L., Ferrara, K. W., Yue, X., & Dai, Z. (2025) · Nano Today · doi:10.1016/j.nantod.2024.102618 · Cited by 14 · see citing works
Kim, W., Kwon, H. J., Jung, H. Y., Hahn, K. R., Yoon, Y. S., Hwang, I. K., Choi, S. Y., & Kim, D. W. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21249496 · Cited by 14 · see citing works
Ogiso, K., Shayo, S. C., Kawade, S., Hashiguchi, H., Deguchi, T., & Nishio, Y. (2022) · PLoS ONE · doi:10.1371/journal.pone.0263080 · Cited by 16 · see citing works
Tripson, M., Litwa, K., & Soderstrom, K. (2023) · Scientific Reports · doi:10.1038/s41598-023-34924-z · Cited by 13 · see citing works
Kulsange, S. E., Sharma, M., Sonawane, B. R., Jaiswal, M. R., Kulkarni, M. J., & Santhakumari, B. (2024) · Food and Chemical Toxicology · doi:10.1016/j.fct.2024.114667 · Cited by 13 · see citing works
Hwang, H. S., & Maeng, Y. (2020) · Stem Cells International · doi:10.1155/2020/8403192 · Cited by 13 · see citing works
Wei, H., Sun, T., Liu, J., Wang, X., Zhao, G., Shi, J., & Chen, Y. (2020) · Journal of Receptors and Signal Transduction · doi:10.1080/10799893.2020.1831535 · Cited by 13 · see citing works
Vascular adenosine monophosphate‐activated protein kinase: Enhancer, brake or both?
Chen, H., Vanhoutte, P. M., & Leung, S. W. S. (2019) · Basic & Clinical Pharmacology & Toxicology · doi:10.1111/bcpt.13357 · Cited by 12 · see citing works
Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain
Semyachkina-Glushkovskaya, O. V., Sokolovski, S. G., Fedosov, I. V., Shirokov, A. A., Наволокин, Н. А., Bucharskaya, A. B., Андреевна, Б. И., Terskov, A. V., Dubrovsky, A. I., Telnova, V., et al. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241813696 · Cited by 11 · see citing works
Torres-Sanchez, A., Torres, G., Estrada, S., Pérez, D., Garcia, C., Milián, M., Velázquez, E., Molina, V., & Delgado, Y. (2024) · Pharmaceuticals · doi:10.3390/ph17060694 · Cited by 11 · see citing works
Panda, E. S., Gautam, A. S., Pandey, S. K., & Singh, R. K. (2024) · Inflammation · doi:10.1007/s10753-024-02199-9 · Cited by 11 · see citing works
López-González, D., Ferrada�s, Y., Araniti, F., Graña, E., Hermida‐Ramón, J. M., González, M. V., Teijeira, M., Rey, M., Reigosa, M. J., & Sánchez‐Moreiras, A. M. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1157309 · Cited by 11 · see citing works
Naima, J., & Ohta, Y. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25021233 · Cited by 10 · see citing works
Varma, M., Bhandari, R., Sarkar, A., Jain, M. K., Paliwal, J. K., Medhi, B., & Kuhad, A. (2024) · Neurochemical Research · doi:10.1007/s11064-024-04191-z · Cited by 10 · see citing works
Gu, C., Guo, T., Chen, X., Zhou, X., & Sun, Y. (2025) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-025-01580-y · Cited by 10 · see citing works
Tsai, Y.-W., Yadav, R., & Liu, H. (2025) · Materials Chemistry and Physics · doi:10.1016/j.matchemphys.2025.130467 · Cited by 9 · see citing works
Aoki, T., Wong, V., Endo, Y., Hayashida, K., Takegawa, R., Okuma, Y., Shoaib, M., Miyara, S. J., Yin, T., Becker, L. B., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-30120-1 · Cited by 9 · see citing works
Nieminen, V., Juntunen, M., Naarala, J., & Luukkonen, J. (2022) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2022.108196 · Cited by 8 · see citing works
Wu, H., Hu, T., Zhang, L., Xia, X., Liu, X., Zhu, Q., Wang, M., Sun, Z., Hao, H., Cui, Y., et al. (2022) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2022.871335 · Cited by 7 · see citing works
Adil, M. S., Parvathagiri, V., Verma, A., Liu, F., Rudraraju, M., Narayanan, S. P., & Somanath, P. R. (2022) · Cells · doi:10.3390/cells11111782 · Cited by 7 · see citing works
Shayeghan, M., Ansari, A. M., Forouzesh, F., & Javidi, M. A. (2022) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2022.109357 · Cited by 7 · see citing works
Seenak, P., Kumphune, S., Prasitsak, T., Nernpermpisooth, N., & Malakul, W. (2022) · Frontiers in Medicine · doi:10.3389/fmed.2022.1039707 · Cited by 7 · see citing works
Lartey, N. L., Vargas‐Robles, H., Guerrero-Fonseca, I. M., Nava, P., Kumatia, E. K., Ocloo, A., & Michael Schnoor, A. (2022) · Immunology Letters · doi:10.1016/j.imlet.2022.07.006 · Cited by 7 · see citing works
Mechanism of Vascular Toxicity in Rats Subjected to Treatment with a Tyrosine Kinase Inhibitor
Reyes‐Goya, C., Garrido, Á. S., Soto-Astacio, E., Aramburu, Ó., Zambrano, S. S., Mate, A., & Vázquez, C. M. (2020) · Toxics · doi:10.3390/toxics8030049 · Cited by 6 · see citing works
Murphy, D. A., Patel, H., & Wimalasena, K. (2021) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.0c00422 · Cited by 6 · see citing works
UVR-induced phototoxicity mechanism of methyl N-methylanthranilate in human keratinocyte cell line
Chandra, S., Qureshi, S., Chopra, D., Shukla, S., Patel, S. K., Singh, J., & Ray, R. S. (2022) · Toxicology in Vitro · doi:10.1016/j.tiv.2022.105322 · Cited by 6 · see citing works
Wani, M. J., Arif, A., Salman, K. A., & Mahmood, R. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.132257 · Cited by 5 · see citing works
Advances in the Study of Vascular Protective Effects and Molecular Mechanisms of Mulberry
Hao, J., Lu, M., Li, J., Guan, X., Yang, W., Sun, R., Zhang, M., Zhao, Y., Jiang, T., & Li, C. (2024) · Food Reviews International · doi:10.1080/87559129.2024.2403523 · Cited by 5 · see citing works
Zhou, K., Wu, G. E., Dong, R., Kan, C., Xie, L., Gao, L., Li, H., Sun, J., & Ning, W. (2025) · Cell Regeneration · doi:10.1186/s13619-025-00242-0 · Cited by 5 · see citing works
Farra, Y. M., Sabnis, S., Matz, J., Wilker, H. I. B., Williams, V. A., Trejo, O., Kim, H. H., Rivera, C. F., Vlahos, J., Ramkhelawon, B., et al. (2025) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.125.320908 · Cited by 4 · see citing works
Jeong, Y., Yang, D., Solidum, J. G. N., Ortinau, L. C., & Park, D. (2025) · Journal of Cellular Physiology · doi:10.1002/jcp.70016 · Cited by 4 · see citing works
Wołosiewicz, M., Balatskyi, V. V., Duda, M. K., Filip, A., Ntambi, J. M., Navrulin, V. O., & Dobrzyń, P. (2024) · Journal of Lipid Research · doi:10.1016/j.jlr.2024.100612 · Cited by 4 · see citing works
Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage
Broderick, M. T., Prince, A. D. P., Dhukhwa, A., Mukherjea, D., Jiang, P., Campbell, K. C. M., Rybak, L. P., & Brenner, M. J. (2021) · Otology & Neurotology · doi:10.1097/mao.0000000000003193 · Cited by 4 · see citing works
(2018) · Methods in molecular biology · doi:10.1007/978-1-4939-7598-3 · Cited by 4 · see citing works
Advancements in Cellular Imaging: Expanding Horizons with Innovative Dyes and Techniques
Oak, P. M., & Mali, A. S. (2024) · Colorants · doi:10.3390/colorants3040025 · Cited by 3 · see citing works
Sirtuin 1 Activation Mitigates Murine Vasculitis Severity by Promoting Mitophagy
Atici, A. E., Jena, P. K., de Carvalho, T. T., Ross, B. E., Aubuchon, E. A., Lane, M. E., Gomez, A. C., Lee, Y., Fishbein, M. C., Azer, J., et al. (2026) · Circulation Research · doi:10.1161/circresaha.125.327991 · Cited by 5 · see citing works
Sytha, S. P., Bray, J., & Heaps, C. L. (2023) · Microvascular Research · doi:10.1016/j.mvr.2023.104590 · Cited by 3 · see citing works
Inhibition of activin A receptor signalling attenuates age-related pathological cardiac remodelling
Clavere, N. G., Alqallaf, A., Rostron, K. A., Parnell, A., Mitchell, R., Patel, K., & Boateng, S. Y. (2022) · Disease Models & Mechanisms · doi:10.1242/dmm.049424 · Cited by 3 · see citing works
Lee, T.-H., Jung, W., Cho, H., & Lee, M.-G. (2019) · Korean Journal of Sport Science · doi:10.24985/kjss.2019.30.3.470 · Cited by 2 · see citing works
Wu, H., Zhu, Q., Liu, X., Hao, H., Sun, Z., Wang, M., Hill, M. A., Xu, C., & Liu, Z. (2022) · Biology · doi:10.3390/biology11081169 · Cited by 2 · see citing works
SEKAR, A., Pandian, A., Veerabathiran, R., Mariappan, R., Chelladurai, G., & Sivamani, G. (2022) · International Journal of Health Sciences · doi:10.53730/ijhs.v6ns4.11408 · Cited by 2 · see citing works
Dolutegravir-induced growth and lifespan effects in Caenorhabditis elegans
Kuo, S., Hsu, W., Wu, C., Lai, Y., & Chen, T. (2023) · BMC Pharmacology and Toxicology · doi:10.1186/s40360-023-00715-5 · Cited by 2 · see citing works
Transcranial photosensitiser-free laser treatment of glioblastoma in rat brainPreprint
SEMYACHKINA-GLUSHKOVSKAYA, O. V., Sokolovski, S. G., Noghero, A., Евгеньевич, Б. Д., Bragina, O. A., Fedosov, I. V., Shirokov, A. A., Наволокин, Н. А., Bucharskaya, A. B., Khorovodov, A. P., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-2666422/v1 · Cited by 2 · see citing works
Singh, K., Kumari, S., Ali, M., Das, M. K., Mishra, A., & Singh, A. K. (2023) · International Journal of Biometeorology · doi:10.1007/s00484-023-02555-8 · Cited by 1 · see citing works
Quantification of mitochondrial reactive oxygen species in macrophages during sepsis
Kanmani, S., Kanmani, P., & Hu, G. (2024) · Methods in cell biology · doi:10.1016/bs.mcb.2024.01.005 · Cited by 1 · see citing works
Takahashi, R., Nojiri, H., Ohara, Y., Fujiwara, T., & Ishijima, M. (2024) · Journal of Orthopaedic Research® · doi:10.1002/jor.25863 · Cited by 1 · see citing works
Techniques to Determine Mammalian Sperm Capacitation
Rodríguez-Gil, J. E., & Blanco‐Prieto, O. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-4406-5_31 · Cited by 1 · see citing works
Space Radiation Sparks Hidden Cancer Risks: The Bystander Effect Unveiled
Aravindan, N., Krishnan, M., Adhikary, A., & Natarajan, M. (2026) · Space Science & Technology · doi:10.34133/space.0518 · Cited by 1 · see citing works
Sensitizer-free photoactivation of singlet oxygen induces glioma cell death
Vaziri, M. R. R., Imanparast, A., Terskov, A. V., Evsukova, A., Sazgarnia, A., & Shirokov, A. A. (2026) · The European Physical Journal Special Topics · doi:10.1140/epjs/s11734-026-02371-2 · Cited by 1 · see citing works
FLOW CYTOMETRY IN NANOTOXICOLOGY: brief overview
Tkachenko, A. S., Onishchenko, A. I., Butov, D., & Tkachenko, M. (2022) · Inter Collegas · doi:10.35339/ic.8.4.278-289 · Cited by 1 · see citing works
- Authors
- Wang, Q., & Zou, M.-H.
- Affiliations
- Georgia State University (US)
- Corresponding author
- Zou, Ming-Hui
- Venue
- Methods in Molecular Biology
- Publisher
- Springer New York
- Published
- 2018
- Volume
- 1732
- Pages
- 507-517
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- PMC
- PMC6407612
- OpenAlex
- W2789049383
- Cited by
- 125
- References
- 18
- Citations by year
- 2026: 10 · 2025: 13 · 2024: 15 · 2023: 19 · 2022: 24 · 2021: 19 · 2020: 18 · 2019: 4 · 2018: 2
- FWCI
- 14.44
- Type
- book chapter
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Aging (R01AG047776)
- National Heart, Lung, and Blood Institute (R01HL105157, R01HL079584, R01HL142287, R01HL137371, R01HL132500, R01HL074399, R01HL080499, R01HL140954, R01HL110488, R01HL128014, R01HL089920, R01HL096032)
- National Cancer Institute (R01CA213022)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- reactive oxygen species, mitochondrial superoxide, superoxide, AMPK, aorta, dihydroethidium, MitoSOX, cardiovascular disease, redox regulation, vascular cells
- MeSH terms
- Animals, Aorta, Cardiovascular Diseases, Chromatography, High Pressure Liquid, Ethidium, Fluorescent Dyes, Microscopy, Fluorescence, Mitochondria, Oxidation-Reduction, Phenanthridines, Reactive Oxygen Species, Mice, Knockout, Myocytes, Smooth Muscle, Endothelial Cells, Mice, AMP-Activated Protein Kinases
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