Cited by 4CRISPR and Genetic EngineeringCarcinogens and Genotoxicity AssessmentPlant Genetic and Mutation Studies

Using Functional Genetics in Haploid Cells for Drug Target Identification

Volz, Jennifer C., Schuller, Nicole, Elling, Ulrich·2019·Methods in Molecular Biology

Abstract

Pooled genetic screens are a powerful tool to identify targets for drug development as well as chemogenetic interactions. Various complementary methods for mutagenesis are available to generate highly complex cell populations, including mRNA knockdown, directed genome editing, as well as random genome mutagenesis. With the availability of a growing number of haploid mammalian cell lines, random mutagenesis is becoming increasingly powerful and represents an attractive alternative, e.g., to CRISPR-based screening. This chapter provides a step-by-step protocol for performing haploid gene trap screens.

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Crossref · 10.1007/978-1-4939-9145-7_1

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Authors
Volz, J. C., Schuller, N., & Elling, U.
Affiliations
Institute of Molecular Biotechnology (AT)
Corresponding author
Elling, Ulrich
Venue
Methods in Molecular Biology
Published
2019
Volume
1953
Pages
3-21
ISSN
  • 1064-3745 (ISSN-L)
  • 1940-6029
OpenAlex
W2942987289
Cited by
4
References
29
Citations by year
2023: 1 · 2022: 2 · 2019: 1
FWCI
0.82
Type
book chapter
Language
en
Open access
closed
Locations
  • Methods in molecular biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
Keywords
haploid cells, drug target identification, random mutagenesis, mRNA knockdown, genome editing, mammalian cell lines
MeSH terms
Animals, Cell Line, Drug Evaluation, Preclinical, Flow Cytometry, Genetic Testing, Haploidy, Humans, Stem Cells, Polymerase Chain Reaction, Mutagenesis, Cell Culture Techniques, Mice, Drug Discovery

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