Using Functional Genetics in Haploid Cells for Drug Target Identification
Abstract
Pooled genetic screens are a powerful tool to identify targets for drug development as well as chemogenetic interactions. Various complementary methods for mutagenesis are available to generate highly complex cell populations, including mRNA knockdown, directed genome editing, as well as random genome mutagenesis. With the availability of a growing number of haploid mammalian cell lines, random mutagenesis is becoming increasingly powerful and represents an attractive alternative, e.g., to CRISPR-based screening. This chapter provides a step-by-step protocol for performing haploid gene trap screens.
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KBM7 cells are a valid model to capture the overall essentiality profile of both ligases.
“KBM7 cells, which are a frequently used tool to study mechanisms of drug resistance are thus a valid model to capture the overall essentiality profile of both ligases. 28–30.”
From Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders (2023) · cites this paper as [29]
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Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders
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- Authors
- Volz, J. C., Schuller, N., & Elling, U.
- Affiliations
- Institute of Molecular Biotechnology (AT)
- Corresponding author
- Elling, Ulrich
- ORCID
- Elling, Ulrich
- Venue
- Methods in Molecular Biology
- Publisher
- Springer New York
- Published
- 2019
- Volume
- 1953
- Pages
- 3-21
- ISSN
- 1064-3745 (ISSN-L)
- 1940-6029
- OpenAlex
- W2942987289
- Cited by
- 4
- References
- 29
- Citations by year
- 2023: 1 · 2022: 2 · 2019: 1
- FWCI
- 0.82
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Methods in molecular biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- haploid cells, drug target identification, random mutagenesis, mRNA knockdown, genome editing, mammalian cell lines
- MeSH terms
- Animals, Cell Line, Drug Evaluation, Preclinical, Flow Cytometry, Genetic Testing, Haploidy, Humans, Stem Cells, Polymerase Chain Reaction, Mutagenesis, Cell Culture Techniques, Mice, Drug Discovery
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